Results 91 to 100 of about 3,754,687 (312)

NAC and DTT promote TGF-β1 monomer formation: demonstration of competitive binding

Journal of Inflammation, 2006
TGF-β plays an important role in the genesis and progression of pulmonary fibrosis. We sought to determine the role of mononuclear phagocytes in the activation of TGF-β and found that freshly isolated peripheral blood monocytes spontaneously released TGF-
Frambach Gwyn, Hunter Melissa, Montague Christine R, Lichtenberger Frank J, Marsh Clay B   +4 more
doaj   +1 more source

Studies on the Binding of Carboxypeptidase A and Several Competitive Inhibitors

Journal of Biological Chemistry, 1962
Several spectral changes have been reported to be related to changes in bonding or other factors in the environment of the side chains of aromatic amino acid residues (l-lo). It would then be expected that on addition of a competitive inhibitor to an enzyme solution, a change in the spectrum of the enzyme could be observed, if aromatic amino acid ...
H, FUJIOKA, K, IMAHORI
openaire   +2 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

A Competitive Nucleotide Binding Inhibitor: In vitro Characterization of Rab7 GTPase Inhibition

ACS Chemical Biology, 2012
Mapping the functionality of GTPases through small molecule inhibitors represents an underexplored area in large part due to the lack of suitable compounds.
J. Agola, Lin Hong, Z. Surviladze, O. Ursu, A. Waller, J. Strouse, D. Simpson, Chad E. Schroeder, Tudor I. Oprea, Jennifer E. Golden, J. Aubé, T. Buranda, L. Sklar, A. Wandinger-Ness   +13 more
semanticscholar   +1 more source

Kinetics of Competitive Binding with Application to Thrombin Complexes

Analytical Biochemistry, 1997
The kinetics of competitive binding is treated analytically, allowing the rate constants to be determined accurately from simple experiments. The method is especially suited to situations where traditional approximations and numerical integration fail, e.g., when the dissociation constants are small or when the concentration of one receptor cannot be ...
W J, Wedemeyer, R W, Ashton, H A, Scheraga   +2 more
openaire   +2 more sources

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Elucidation and Structural Modeling of CD71 as a Molecular Target for Cell-Specific Aptamer Binding

Journal of the American Chemical Society, 2019
Pancreatic cancer is a highly lethal malignancy associated with tissues of the pancreas. Early diagnosis and effective treatment are crucial to improving the survival rate of patients with pancreatic cancer.
Xiaoqiu Wu, Honglin Liu, Dongmei Han, Bo Peng, Hui Zhang, Lin Zhang, Jianglin Li, Jing Liu, Cheng Cui, S. Fang, Min Li, Mao Ye, W. Tan   +12 more
semanticscholar   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Functional Methods for Studying Sperm–Zona Pellucida Interaction in Mammals

Methods and Protocols
The initial molecular events mediating mammalian sperm binding to the zona pellucida (ZP) of the oocyte are highly complex and still not fully elucidated.
Natalie Zelenkova, Veronika Kraus, Alexandra Maresova, Zuzana Pilsova, Aneta Pilsova, Barbora Klusackova, Eva Chmelikova, Katerina Komrskova, Pavla Postlerova   +8 more
doaj   +1 more source