Results 101 to 110 of about 2,748,278 (304)

Investigate synergistic and competitive regulation between miRNAs by using a multivariate information measurement

BMC Bioinformatics
Multiple microRNAs can bind to the 3’ untranslated region (3’UTR) of the same mRNA transcript. Depending on the spatial arrangement of their binding sites, endogenous microRNAs may cooperate to enhance repression or compete for overlapping sites ...
Chu Pan, Yanlin Chen, Tianwu Zhang
doaj   +1 more source

Reprogramming tumor cells with LCOR mRNA nanotherapy to enhance immunotherapy

Molecular Oncology, EarlyView.
LCOR (Ligand‐dependent corepressor) mRNA encapsulated in polymeric nanoparticles restores LCOR activity in tumor cells, constraining aggressive tumor cell states and increasing tumor immunogenicity. This reprogramming makes tumor cells more vulnerable to T‐cell attack and enhances their response to immune‐checkpoint immunotherapy.
Gabriel Serra‐Mir, Elena Haro‐Martínez, Sandra Blasco‐Benito, Chiara Cannatà, José Ángel Palomeque, Anna López‐Plana, Maria Sanz‐Flores, Marc Sabartés, Cristina Fornaguera, Toni Celià‐Terrassa   +9 more
wiley   +1 more source

Competitive binding of monoclonal antibodies.

, 2012
Each monoclonal antibody was labeled with horseradish peroxidase (HRPO). The ability of a large excess of each unlabeled antibody to inhibit the binding of HRPO MAbs was measured.
Gerald R. Nakamura (158427), Aaron L. Vollrath (158433), Sara M. O'Rourke (158431), Phillip W. Berman (158435), Dora P. A. J. Fonseca (158429)   +4 more
core   +1 more source

Ligand-binding properties of XaffOBP9, a Minus-C odorant-binding protein from Xyleborus affinis (Coleoptera: Curculionidae: Scolytinae)

Frontiers in Physiology
Xyleborus affinis, one of the most important pests of rubber trees, has caused severe damage to the natural rubber industry in Hainan province. The ability to detect host plants through a sensitive and specific olfactory system is crucial for Xyleborus ...
Qian Wang, Xiang Zhou, Kai Zhang, Lei Qin, Qi Wu, Linan Deng, Zheyuan Xu, Jixing Guo   +7 more
doaj   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Peptide aptamers define distinct EB1- and EB3-binding motifs and interfere with microtubule dynamics [PDF]

, 2014
EB1 is a conserved protein that plays a central role in regulating microtubule dynamics and organisation. It binds directly to microtubule plus ends and recruits other plus end localising proteins.
Warbrick, Emma, Ohkura, Hiroyuki, Lesniewska, Karolina   +2 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Design, synthesis, and evaluation of a novel fluorescent probe for competitive fluorescence polarization assay to screen galectin-8 inhibitors

Acta Pharmaceutica
In the present work, we describe the design, synthesis, and evaluation of a galectin-8-binding fluorescent probe designed for a competitive fluorescence polarization (FP) assay for screening new galectin-8N inhibitors. The probe was characterized for its
Purić Edvin, Marinović Marina, Kojek Zala, Kahl-Knutson Barbro, Leffler Hakon, Nilsson Ulf J., Anderluh Marko, Mravljak Janez   +7 more
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Dual enzyme labels for simultaneous heterogeneous enzyme-linked competitive binding assays

, 1994
The use of two enzyme labels in a dual analyte simultaneous heterogeneous enzyme-linked competitive binding assay is examined. Reaction conditions for monitoring glucose-6-phosphate dehydrogenase (G6PDH) and [beta]-galactosidase ([beta]-gal) activities ...
Buckwalter, Jeanette M., Meyerhoff, Mark E., Guo, Xuan   +2 more
core   +1 more source