Results 251 to 260 of about 3,754,687 (312)

ASSAY OF ALDOSTERONE BY COMPETITIVE PROTEIN BINDING

Acta Endocrinologica, 1970
ABSTRACT We have applied a competitive protein binding technique to the assay of aldosterone, utilizing the aldosterone binding protein (ABP) in kidney as the specific receptor molecule. Intracellular ABP was obtained from the homogenization of adrenalectomized rat kidneys in 0.25 M sucrose.
R G, Robinson, D D, Fanestil
openaire   +2 more sources

Competitive Protein Binding Assay for Piritrexim

Journal of Pharmaceutical Sciences, 1989
A competitive protein binding assay for piritrexim (PTX, 1) that makes use of a commercially available radioassay kit for methotrexate has been developed. After it is selectively extracted from plasma, PTX competes with [125l]methotrexate for binding to dihydrofolate reductase isolated from Lactobacillus casei. Free drug is separated from bound drug by
J L, Woolley, J L, Ringstad, C W, Sigel
openaire   +2 more sources

Determination of calmodulin by competitive binding assay

Analytical Biochemistry, 1986
Calmodulin levels in tissue or cellular extracts can be determined by competition with 125I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a
H, LeVine, N, Sahyoun, P, Cuatrecasas
openaire   +2 more sources

Measurement of thyroxine by competitive protein binding

Clinica Chimica Acta, 1972
Abstract A simplified method is described for the measurement of thyroxine by competitive protein binding, using Sephadex columns for separation of bound from unbound thyroxine. Conditions for the various steps in the method were studied. The results compare well with PBI determinations done on the same subjects.
H, Seligson, D, Seligson
openaire   +2 more sources

Competitive protein binding assay for 24,25-dihydroxycholecalciferol

Biochemical and Biophysical Research Communications, 1976
Abstract A competitive protein binding assay which measures 24,25-dihydroxycholecalciferol in human serum has been developed using the binding protein from vitamin D-deficient rat kidney. As 25-hydroxycholecalciferol and 25,26-dihydroxycholecalciferol also interact with the binding protein, possible interference by these compounds in the assay has ...
C M, Taylor, S E, Hughes, P, de Silva
openaire   +3 more sources

The Problem of Partial Competition in the Quantitative Characterization of Interactions by Competitive Binding Assays

Analytical Biochemistry, 1993
Binding expressions are derived and analytical procedures developed for the quantitative characterization of inhibitor binding that is only partially competitive with the interaction between an acceptor and the ligand that is being monitored. Two such situations are considered: (i) that in which the partial competition reflects binding of inhibitor to ...
Brocklebank, Anne M., Sawyer, William H., Wiley, James S., Winzor, Donald J.   +3 more
openaire   +4 more sources

Bargaining, binding contracts, and competitive wages

Games and Economic Behavior, 2003
In a model where many workers bargain with one firm and sign binding contracts, the existence of a stationary subgame perfect equilibrium is shown. If the production function satisfies decreasing returns, each worker receives a share of his marginal product (treating all other workers as employed) in equilibrium. Thus, wages are competitive. This is in
openaire   +2 more sources

Competitive Binding of Aroma Compounds by β-Cyclodextrin

Journal of Agricultural and Food Chemistry, 2001
Retention of six aroma compounds has been studied after dehydration of ternary mixtures of aroma water and beta-cyclodextrin. A maximal retention of a mole of aroma per mole of beta-cyclodextrin has been observed for five of the aroma compounds, whereas retention of benzyl alcohol can be twice as high. Retention of a mixture of aroma compounds has also
Goubet, I., Dahout, C., Semon, Etienne, Guichard, E., Le Quéré, Jean-Luc, Voilley, Andrée   +5 more
openaire   +3 more sources

Competitive binding at the noradrenaline storage sites

Irish Journal of Medical Science, 1966
Cocaine, phenoxybenzamine (Dibenzyline) and, less effectively, phentolamine (Regitine) block the chronotropic response (beta-receptor) of tyramine on the isolated guinea-pig’s atria. Phenoxybenzamine induces a persistant blockade, the others a transitory one as they can readily be removed by washing the preparation.
openaire   +2 more sources

The specificity of asciminib, a potential treatment for chronic myeloid leukemia, as a myristate-pocket binding ABL inhibitor and analysis of its interactions with mutant forms of BCR-ABL1 kinase.

Leukemia research : a Forum for Studies on Leukemia and Normal Hemopoiesis, 2020
Asciminib is a potent, orally bioavailable, investigational drug that specifically and potently inhibits the tyrosine kinase activity of native ABL1, together with that of the chimeric BCR-ABL1 oncoprotein which causes chronic myeloid leukemia (CML).
P. Manley, Louise Barys, S. Cowan-Jacob
semanticscholar   +1 more source