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Determination of calmodulin by competitive binding assay
Analytical Biochemistry, 1986Calmodulin levels in tissue or cellular extracts can be determined by competition with 125I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a
H, LeVine, N, Sahyoun, P, Cuatrecasas
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Measurement of thyroxine by competitive protein binding
Clinica Chimica Acta, 1972Abstract A simplified method is described for the measurement of thyroxine by competitive protein binding, using Sephadex columns for separation of bound from unbound thyroxine. Conditions for the various steps in the method were studied. The results compare well with PBI determinations done on the same subjects.
H, Seligson, D, Seligson
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Bargaining, binding contracts, and competitive wages
Games and Economic Behavior, 2003In a model where many workers bargain with one firm and sign binding contracts, the existence of a stationary subgame perfect equilibrium is shown. If the production function satisfies decreasing returns, each worker receives a share of his marginal product (treating all other workers as employed) in equilibrium. Thus, wages are competitive. This is in
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Competitive protein binding assay for 24,25-dihydroxycholecalciferol
Biochemical and Biophysical Research Communications, 1976Abstract A competitive protein binding assay which measures 24,25-dihydroxycholecalciferol in human serum has been developed using the binding protein from vitamin D-deficient rat kidney. As 25-hydroxycholecalciferol and 25,26-dihydroxycholecalciferol also interact with the binding protein, possible interference by these compounds in the assay has ...
C M, Taylor, S E, Hughes, P, de Silva
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Analytical Biochemistry, 1993
Binding expressions are derived and analytical procedures developed for the quantitative characterization of inhibitor binding that is only partially competitive with the interaction between an acceptor and the ligand that is being monitored. Two such situations are considered: (i) that in which the partial competition reflects binding of inhibitor to ...
Brocklebank, Anne M. +3 more
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Binding expressions are derived and analytical procedures developed for the quantitative characterization of inhibitor binding that is only partially competitive with the interaction between an acceptor and the ligand that is being monitored. Two such situations are considered: (i) that in which the partial competition reflects binding of inhibitor to ...
Brocklebank, Anne M. +3 more
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Competitive binding at the noradrenaline storage sites
Irish Journal of Medical Science, 1966Cocaine, phenoxybenzamine (Dibenzyline) and, less effectively, phentolamine (Regitine) block the chronotropic response (beta-receptor) of tyramine on the isolated guinea-pig’s atria. Phenoxybenzamine induces a persistant blockade, the others a transitory one as they can readily be removed by washing the preparation.
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Competitive Binding of Aroma Compounds by β-Cyclodextrin
Journal of Agricultural and Food Chemistry, 2001Retention of six aroma compounds has been studied after dehydration of ternary mixtures of aroma water and beta-cyclodextrin. A maximal retention of a mole of aroma per mole of beta-cyclodextrin has been observed for five of the aroma compounds, whereas retention of benzyl alcohol can be twice as high. Retention of a mixture of aroma compounds has also
Goubet, I. +5 more
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Cooperation and competition by RNA-binding proteins in cancer
Seminars in Cancer Biology, 2022Post-transcriptional regulation of gene expression plays a major role in determining the cellular proteome in health and disease. Post-transcriptional control mechanisms are disrupted in many cancers, contributing to multiple processes of tumorigenesis. RNA-binding proteins (RBPs), the main post-transcriptional regulators, often show altered expression
Sharanya, Nag +3 more
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Competitive and noncompetitive reversible binding processes
Physical Review E, 1993This work treats many-body aspects in an idealized class of reversible binding problems involving a static binding site with many diffusing point particles. In the noncompetitive limit, where no restriction exists on the number of simultaneously bound particles, the problem reduces to reversible aggregation.
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Modeling binding equilibrium in a competitive estrogen receptor binding assay
Chemosphere, 2007Although the free concentration is more significant in the environmental chemistry and toxicology of receptor-mediated toxicants, few studies have been conducted to use it as a dose-metric. The relative binding affinity of three model endocrine disrupting compounds, diethylstilbestrol (DES), ethynylestradiol (EE2), and bisphenol A (BPA), were evaluated
Jung-Hwan, Kwon +2 more
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