Results 261 to 270 of about 3,754,687 (312)

Modeling binding equilibrium in a competitive estrogen receptor binding assay

Chemosphere, 2007
Although the free concentration is more significant in the environmental chemistry and toxicology of receptor-mediated toxicants, few studies have been conducted to use it as a dose-metric. The relative binding affinity of three model endocrine disrupting compounds, diethylstilbestrol (DES), ethynylestradiol (EE2), and bisphenol A (BPA), were evaluated
Jung-Hwan, Kwon, Lynn E, Katz, Howard M, Liljestrand   +2 more
openaire   +2 more sources

Cooperation and competition by RNA-binding proteins in cancer

Seminars in Cancer Biology, 2022
Post-transcriptional regulation of gene expression plays a major role in determining the cellular proteome in health and disease. Post-transcriptional control mechanisms are disrupted in many cancers, contributing to multiple processes of tumorigenesis. RNA-binding proteins (RBPs), the main post-transcriptional regulators, often show altered expression
Sharanya, Nag, Binita, Goswami, Sukhen, Das Mandal, Partho Sarothi, Ray   +3 more
openaire   +2 more sources

COMPETITION BY 24,25-DIHYDROXYCHOLECALCIFEROL IN THE COMPETITIVE PROTEIN BINDING RADIOASSAY OF 25-HYDROXYCALCIFEROL

The Journal of Clinical Endocrinology & Metabolism, 1976
24,25-dihydroxycholecalciferol (24,25-(OH)2D3) is equipotent to 25-hydroxycholecalciferol in the displacement of 3H25-OHD3 from rat serum binding sites, and is extracted by the ethanol procedure recommended in non-chromatographic competitive protein binding radioassays for 25-OHD.
J G, Haddad, C, Min, J, Walgate, T, Hahn
openaire   +2 more sources

Competitive and noncompetitive reversible binding processes

Physical Review E, 1993
This work treats many-body aspects in an idealized class of reversible binding problems involving a static binding site with many diffusing point particles. In the noncompetitive limit, where no restriction exists on the number of simultaneously bound particles, the problem reduces to reversible aggregation.
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A direct competitive binding radioimmunoassay for carcinoembryonic antigen

Journal of Immunological Methods, 1980
We have incorporated commercially available CEA standard and antiserum into the triple isotope double antibody radioimmunoassay and we have evaluated this assay for the routine determination of CEA. The competitive protein binding (CPB) assay for CEA can be performed directly on serum or plasma without perchloric acid extraction.
H A, Fritsche, C K, Tashima, W L, Collinsworth, A, Geitner, J, Van Oort   +4 more
openaire   +2 more sources

Adrenocorticotropin Measurement by Competitive Binding Receptor Assay

The Journal of Clinical Endocrinology & Metabolism, 1972
A competitive binding assay using the cortical receptor from normal adrenal glands has been developed for ACTH. In this assay porcine, human or rabbit ACTH has been shown to compete with labeled porcine ACTH for the cortical receptor from ovine, rabbit or rat adrenal glands.
A R, Wolfsen, H B, McIntyre, W D, Odell
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Plasma progesterone and competitive protein binding assay

American Journal of Obstetrics and Gynecology, 1971
Abstract A brief review of the current status of the assay of plasma progesterone, particularly as it relates to the competitive protein binding ...
B D, Reeves, E, Diczfalusy
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Analyzing competitive binding data

2004
Abstract Competitive binding experiments measure the binding of a single concentration of labeled ligand in the presence of various concentrations of unlabeled ligand. Ideally, the concentration of unlabeled ligand varies over at least six orders of magnitude.
Harvey Motulsky, Arthur Christopoulos
openaire   +1 more source

Homologous competitive binding curves

2004
Abstract The most common way to determine receptor number ,, nd affinity is to perform a saturation binding experiment, where you vary the concentration of radioligand. An alternative is to keep the radioligand concentration constant, and compete for binding with the same ligand, but not radioactively labeled.
Harvey Motulsky, Arthur Christopoulos
openaire   +1 more source

Competitive protein-binding radioassay for retinoic acid

Analytical Biochemistry, 1980
Rat testes cytosol treated by Blue Sepharose was employed in a simple and sensitive method for the determination of retinoic acid in the rat serum, liver, and intestine. The method permits the detection of as little as 3 ng of retinoic acid. The mean concentrations of retinoic acid in normal male rats were 33.5 ng/ml of serum, 624.9 ng/g wet wt of ...
Y, Shidoji, N, Hosoya
openaire   +2 more sources