Results 261 to 270 of about 2,617,421 (301)

COMPETITION BY 24,25-DIHYDROXYCHOLECALCIFEROL IN THE COMPETITIVE PROTEIN BINDING RADIOASSAY OF 25-HYDROXYCALCIFEROL

The Journal of Clinical Endocrinology & Metabolism, 1976
24,25-dihydroxycholecalciferol (24,25-(OH)2D3) is equipotent to 25-hydroxycholecalciferol in the displacement of 3H25-OHD3 from rat serum binding sites, and is extracted by the ethanol procedure recommended in non-chromatographic competitive protein binding radioassays for 25-OHD.
J G, Haddad, C, Min, J, Walgate, T, Hahn
openaire   +2 more sources

Characterizing Ligand-Microtubule Binding by Competition Methods

2007
The knowledge of the thermodynamics and kinetics of drug-microtubule interaction is essential to understand the structure/affinity relationship of a given ligand family. When a ligand does not show an appropriate signal change (absorbance or fluorescence) upon binding, the extensive direct characterization of its binding affinities and kinetic rate ...
Díaz, José Fernando, Martínez Buey, Rubén   +1 more
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Analyzing competitive binding data

2004
Abstract Competitive binding experiments measure the binding of a single concentration of labeled ligand in the presence of various concentrations of unlabeled ligand. Ideally, the concentration of unlabeled ligand varies over at least six orders of magnitude.
Harvey Motulsky, Arthur Christopoulos
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Competitive protein-binding radioassay for retinoic acid

Analytical Biochemistry, 1980
Rat testes cytosol treated by Blue Sepharose was employed in a simple and sensitive method for the determination of retinoic acid in the rat serum, liver, and intestine. The method permits the detection of as little as 3 ng of retinoic acid. The mean concentrations of retinoic acid in normal male rats were 33.5 ng/ml of serum, 624.9 ng/g wet wt of ...
Y, Shidoji, N, Hosoya
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Competitive Binding Among Oral Streptococci to Hydroxyapatite

Journal of Dental Research, 1977
The relative affinity of various oral streptococci for hydroxyapatite (HA) differed widely. Streptococcus mutans affinity for dextran-coated HA was the highest of all the streptococci to any HA coating. S salivarius had the lowest affinity, and S sanguis affinity was higher than S miteor for saliva-coated HA.
W F, Liljemark, S V, Schauer
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Homologous competitive binding curves

2004
Abstract The most common way to determine receptor number ,, nd affinity is to perform a saturation binding experiment, where you vary the concentration of radioligand. An alternative is to keep the radioligand concentration constant, and compete for binding with the same ligand, but not radioactively labeled.
Harvey Motulsky, Arthur Christopoulos
openaire   +1 more source

A direct competitive binding radioimmunoassay for carcinoembryonic antigen

Journal of Immunological Methods, 1980
We have incorporated commercially available CEA standard and antiserum into the triple isotope double antibody radioimmunoassay and we have evaluated this assay for the routine determination of CEA. The competitive protein binding (CPB) assay for CEA can be performed directly on serum or plasma without perchloric acid extraction.
H A, Fritsche, C K, Tashima, W L, Collinsworth, A, Geitner, J, Van Oort   +4 more
openaire   +2 more sources

Competitive binding of bilirubin to albumin

1971
Stern, L. (Supervisor)
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Inhibition of radioligand binding to receptors: a competitive business

Trends in Pharmacological Sciences, 1988
Abstract Competitive inhibition of radioligand binding is most simply described as the mutually exclusive binding of two compounds to a common site on a receptor. George Tomlinson argues that apparent competitive inhibition is to be expected for all inhibitory systems, including those involving allosteric (negatively cooperative) interactions ...
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Zona Binding: Competitive Sperm-Binding Assay

2021
De Yi Liu, Ying Zhong, Zi-Na Wen
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