Results 261 to 270 of about 1,526,997 (293)

Distribution of Antibody-Binding Affinity

The Journal of Immunology, 1974
Abstract It has been shown that when a direct binding assay, such as equilibrium dialysis, is used to measure antibody site concentration an underestimate of the concentration of low affinity antibodies may occur in the presence of small amounts of high affinity antibody.
Young Tai Kim, Theodore P Werblin, Gregory W Siskind   +2 more
openaire   +1 more source

α2-macroglobulin binding to cultured fibroblasts: Identification by affinity chromatography of high-affinity binding sites

Archives of Biochemistry and Biophysics, 1983
Binding sites having the properties of high-affinity receptors for activated alpha 2-macroglobulin (alpha 2M) have been purified over 100-fold from membranes of spontaneously transformed NIH-3T3 cells (J. A. Hanover, S.-y. Cheng, M. C. Willingham, and I. H. Pastan [1983] J. Biol. Chem. 258, 370-377).
J A, Hanover, J E, Rudick, M C, Willingham, I, Pastan   +3 more
openaire   +2 more sources

Thermostable carbohydrate‐binding modules in affinity chromatography

Journal of Molecular Recognition, 2006
AbstractAffinity chromatography is routinely used mostly on a preparative scale to isolate different biomolecules such as proteins and carbohydrates. To this end a variety of proteins is in common use as ligands. To extend the arsenal of binders intended for separation of carbohydrates, we have explored the use of carbohydrate‐binding modules (CBM) in ...
Reine, Johansson, Lavinia Cicortas, Gunnarsson, Mats, Ohlin, Sten, Ohlson   +3 more
openaire   +2 more sources

An affinity column for ecdysone binding proteins

Analytical Biochemistry, 1976
Abstract Under basic conditions β-ecdysone can be covalently linked to the oxirane residue of epoxy-activated Sepharose 6B. Such an ecdysone-modified column matrix retards antibodies to β-ecdysone while permitting, however, the free passage of other antibodies in rabbit antiserum. The bound anti-ecdysone antibodies can subsequently be eluted by a low
B A, Sage, J D, O'Connor
openaire   +2 more sources

DeepDTAF: a deep learning method to predict protein–ligand binding affinity

Briefings in Bioinformatics, 2021
Yaohang Li, Gaoshi Li
exaly  

Binding Affinity

2013
openaire   +1 more source

High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor

Nature, 1991
Luis F Parada, Moses V Chao
exaly  

Binding Affinity

2006
openaire   +1 more source

Cell surface, heparin-like molecules are required for binding of basic fibroblast growth factor to its high affinity receptor

Cell, 1991
Jeffrey D Esko, David M Ornitz
exaly  

Binding Affinity

2011
openaire   +1 more source