Results 261 to 270 of about 1,037,789 (311)

Dihydrotestosterone derivatives: relative binding affinity versus affinity purification

Steroids, 1991
Mono esters of a homologous series of diacids of dihydrotestosterone were synthesized and converted to the corresponding n-butyl amides. The relative binding affinities of these amides to androgen receptor were compared with the degree of purification of rat prostate androgen receptor by affinity columns prepared by linking the steroidal acid to amino ...
M E, Stobaugh, R T, Blickenstaff
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Assays to Estimate the Binding Affinity of Aptamers

Talanta, 2022
Aptamers have become coming-of-age molecular recognition elements in both diagnostic and therapeutic applications. Generated by SELEX, the 'quality control' of aptamers, which involves the validation of their binding affinity against their respective targets is pivotal to ascertain their potency prior to use in any downstream assays or applications ...
Ramesh, Thevendran, Marimuthu, Citartan
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Binding Affinities of Thyroxine-Binding Proteins in Turtle Plasma

General and Comparative Endocrinology, 1993
Binding affinities (Ka) for thyroxine (T4) by blood plasma and purified plasma proteins from two turtles, the slider (Trachemys scripta) and snapper (Chelydra serpentina), were compared with those of a human using equilibrium dialysis. The purified T4 binding protein (TBP) from T.
K A, Glennemeier, P, Licht
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Quantification of the binding affinity of a specific hydroxyapatite binding peptide

Biomaterials, 2010
The genesis of bone and teeth involves highly coordinated processes, which involve multiple cell types and proteins that direct the nucleation and crystallization of inorganic hydroxyapatite (HA). Recent studies have shown that peptides mediate the nucleation process, control HA microstructure or even inhibit HA mineralization.
Michael C, Weiger, Jung Jin, Park, Marc D, Roy, Christopher M, Stafford, Alamgir, Karim, Matthew L, Becker   +5 more
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Bisphenol A Binds to the Low-Affinity Estrogen Binding Site

In Vitro & Molecular Toxicology, 2001
Environmental estrogens are suspected of being involved in the current increase in the incidence of human reproductive malfunctions, such as a decrease in male reproductive capacity and an increased incidence of breast cancer in women. The influences of these compounds have been proposed to be mediated through binding to macromolecules, such as ...
W, Washington, L, Hubert, D, Jones, W G, Gray   +3 more
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Affinity of Carumonam for Penicillin-Binding Proteins

Chemotherapy, 2009
Direct labeling experiments with [14C]-carumonam as well as competition binding assays with [14C]-benzylpenicillin and [14C]-ceftriaxone have demonstrated that penicillin-binding protein (PBP) 3 of Escherichia coli, Enterobacter cloacae and Pseu-domonas aeruginosa has the highest affinity for carumonam (Ro 17–2301, AMA-1080).
R L, Then, I, Kohl
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An affinity column for ecdysone binding proteins

Analytical Biochemistry, 1976
Abstract Under basic conditions β-ecdysone can be covalently linked to the oxirane residue of epoxy-activated Sepharose 6B. Such an ecdysone-modified column matrix retards antibodies to β-ecdysone while permitting, however, the free passage of other antibodies in rabbit antiserum. The bound anti-ecdysone antibodies can subsequently be eluted by a low
B A, Sage, J D, O'Connor
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Taba: A Tool to Analyze the Binding Affinity

Journal of Computational Chemistry, 2019
Evaluation of ligand‐binding affinity using the atomic coordinates of a protein‐ligand complex is a challenge from the computational point of view. The availability of crystallographic structures of complexes with binding affinity data opens the possibility to create machine‐learning models targeted to a specific protein system. Here, we describe a new
Amauri Duarte da Silva, Gabriela Bitencourt-Ferreira, Walter F. de Azevedo Jr.   +2 more
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Affinity purification of DNA-binding proteins

Journal of Biochemical and Biophysical Methods, 2001
The focus of this review is on DNA affinity chromatography, which is the most powerful tool for purification of DNA binding proteins. The use of nonspecific-, sequence specific- and single stranded-DNA affinity columns in purification of various DNA binding proteins is discussed.
H, Gadgil, S A, Oak, H W, Jarrett
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Affinity probes for the avermectin binding proteins

Journal of Medicinal Chemistry, 1992
The design and synthesis of a series of avermectin affinity probes used in the identification and purification of the avermectin binding proteins is described. These modified avermectins fall into two design classes: ligands to covalently modify specific avermectin binding proteins [an 125I-labeled aryl azide photoprobe (15) and a tritiated aziridine ...
P T, Meinke, S P, Rohrer, E C, Hayes, J M, Schaeffer, M H, Fisher, H, Mrozik   +5 more
openaire   +2 more sources