Results 251 to 260 of about 2,892,311 (358)

Studies of the membrane-bound flavocytochrome MsrQ flavin mononucleotide (FMN)-binding site reveal an unexpected ubiquinone cofactor. [PDF]

FEBS J
Carpentier P, Pierrel F, Duraffourg N, Guigliarelli B, Hajj Chehade M, Flandrin L, Basset C, Caux C, Torelli S, Nivière V.   +9 more
europepmc   +1 more source

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

Role of the CTCF binding site in Human T-Cell Leukemia Virus-1 pathogenesis. [PDF]

PLoS Pathog
Joseph A, Cheng X, Harding J, Al-Saleem J, Green P, Griffith M, Veis D, Rauch DA, Ratner L.   +8 more
europepmc   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Benchmarking transcription factor binding site prediction models: a comparative analysis on synthetic and biological data. [PDF]

Brief Bioinform
Tognon M, Kumbara A, Betti A, Ruggeri L, Giugno R.   +4 more
europepmc   +1 more source

Tryptic Cleavage of Antibody Binding Sites from Hepatitis B Surface Antigen Particles

, 1976
C. J. Burrell, G. Leadbetter, P. Mackay, B. P. Marmion   +3 more
openalex   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Inherent fast inactivation particle of Nav channels as a new binding site for a neurotoxin. [PDF]

EMBO J
Zhou X, Chen H, Peng S, Si Y, Wang G, Yang L, Zhou Q, Lu M, Xie Q, He X, Wu M, Xiao X, Luo X, Feng X, Wang W, Luo S, Li Y, Qin J, Chen M, Zhang Q, Hu W, Liang S, Hou T, Liu Z.   +23 more
europepmc   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Colchicine Binding Site Tubulin Inhibitors Impair Vincristine-Resistant Neuroblastoma Cell Function. [PDF]

Molecules
Reed CN, Garrison KB, Thammathong J, Cinatl J, Michaelis M, Banerjee S, Weissmiller AM.   +6 more
europepmc   +1 more source