Results 291 to 300 of about 1,627,769 (335)

Antibody Binding Sites

1996
This chapter assesses advances in the understanding and manipulation of antibody binding sites and variable regions. The antigen binding region of an antibody reduced to a 25-kDa minimum comprises of a noncovalent heterodimer of V H and V L domains.
J S, Huston, M N, Margolies, E, Haber
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Photoactivable Antibody Binding Protein: Site-Selective and Covalent Coupling of Antibody

Analytical Chemistry, 2009
Here we report new photoactivable antibody binding proteins, which site-selectively capture antibodies and form covalent conjugates with captured antibodies upon irradiation. The proteins allow the site-selective tagging and/or immobilization of antibodies with a highly preferred orientation and omit the need for prior antibody modifications.
Yongwon, Jung, Jeong Min, Lee, Jung-won, Kim, Jeongwon, Yoon, Hyunmin, Cho, Bong Hyun, Chung   +5 more
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Mapping antibody binding sites on protein antigens

Nature, 1992
The large surfaces of protein antigens that interact with antibody-combining sites can be determined using deuterium-exchange labelling and two-dimensional 1H nuclear magnetic resonance (NMR). This technique may also be applied to other protein-protein interactions to identify key residues that contribute to the affinity.
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Binding site of the rat liver specific monoclonal antibody

Biochemical and Biophysical Research Communications, 1988
A rat liver-specific antigen (RLSA) lost its binding ability to the corresponding monoclonal antibody after treatment with N-glycanase or sialidase, which suggested that the specific binding site might be in a portion of the sugar chain containing sialic acid.
K, Sato, T, Ikeda, Y, Osada
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Antibody-antigen Interactions: Contact Analysis and Binding Site Topography

Journal of Molecular Biology, 1996
We have analysed antigen-contacting residues and combining site shape in the antibody Fv and Fab crystal structures now available from the Protein Data Bank. Antigen-contacting propensities are presented for each antibody residue, allowing a new definition for the complementarity determining regions (CDRs) to be proposed based on observed antigen ...
R M, MacCallum, A C, Martin, J M, Thornton   +2 more
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A model for a glutamate receptor agonist antibody-binding site

Journal of Molecular Recognition, 1999
A combination of mutagenesis, computer modeling and immunoreactivity has been used to develop a structural model of a segment of the glutamate receptor (GluR), termed GluR3B, which is bound by receptor-activating autoantibodies. In this model, the GluR3B epitope is located in a reverse hairpin loop that places key residues important for antibody ...
S, McDonald, N G, Carlson, L C, Gahring, K R, Ely, S W, Rogers   +4 more
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Ligand Binding to Anti-Fluorescyl Antibodies: Stability of the Antigen Binding Site

Biochemistry, 1994
The problem of protein stability is addressed with spectroscopic studies of equilibrium and kinetic properties of the binding of fluorescein to high-affinity monoclonal anti-fluorescyl antibodies (Mab 4-4-20), Fab fragments, and single-chain antibodies (SCA). SCA molecules contain only the variable domains of the antibody and an amino acid linker.
Müller, J. D., Nienhaus, G. U., Tetin, S. Y., Voss, E. W.   +3 more
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Binding Sites of Monoclonal Anti-Carbohydrate Antibodies

1988
This chapter presents an overview of monoclonal antibodies (mAbs) directed to carbohydrates in mammalian systems with well-characterized specificities, together with some of the information obtained from their use. Because of the rapidly increasing number of this kind of reagent not all available mAbs are included.
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Antigen-specific anti-phosphocholine antibodies: binding site studies.

The Journal of Immunology, 1985
Abstract The present investigation extends our initial evaluation of the evolution of antigen selection mechanisms for antibodies of a "single" specificity. The binding sites of 11 mouse anti-PC antibodies produced in response to the bacterium P. morganii or the nematode A.
J L, Claflin, S, Hudak, A, Maddalena, T, Bender   +3 more
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Antigen-binding sites dominate the surface properties of IgG antibodies

Molecular Immunology, 1995
A new technique, liquid-liquid partition chromatography in an aqueous polyethylene glycol-dextran two-phase system, was used to detect differences in surface properties of antibodies with different antigen-binding sites. Employing well-characterized monoclonal IgG antibodies and Fab and Fc fragments thereof as well as chimeric IgG antibodies we found a
C, Wingren, U B, Hansson, C G, Magnusson, M, Ohlin   +3 more
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