Results 271 to 280 of about 675,780 (316)

Structure, function and properties of antibody binding sites

Journal of Molecular Biology, 1991
Do antibody combining sites possess general properties that enable them to bind different antigens with varying affinities and to bind novel antigens? Here, we address this question by examining the physical and chemical characteristics most favourable for residues involved in antigen accommodation and binding.
I S, Mian, A R, Bradwell, A J, Olson
openaire   +2 more sources

Specific suppression by anti-binding site antibody

Cellular Immunology, 1975
Heterologous antisera against the binding site of different phosphorylcholine BALB/c myeloma proteins, TEPC-15, MOPC-167, and McPC-603, were prepared by absorption on TEPC-15-Sepharose in the presence of hapten. The binding of the absorbed anti-TEPC-15 to 125I-(Fab)2 TEPC-15 is inhibitable by unlabeled phosphorylcholine myeloma proteins and by hapten ...
Kohler, H, Smyk, S, Kluskens, L
openaire   +2 more sources

A model for a glutamate receptor agonist antibody-binding site

Journal of Molecular Recognition, 1999
A combination of mutagenesis, computer modeling and immunoreactivity has been used to develop a structural model of a segment of the glutamate receptor (GluR), termed GluR3B, which is bound by receptor-activating autoantibodies. In this model, the GluR3B epitope is located in a reverse hairpin loop that places key residues important for antibody ...
S, McDonald, N G, Carlson, L C, Gahring, K R, Ely, S W, Rogers   +4 more
openaire   +2 more sources

Antibody Binding Sites

1996
This chapter assesses advances in the understanding and manipulation of antibody binding sites and variable regions. The antigen binding region of an antibody reduced to a 25-kDa minimum comprises of a noncovalent heterodimer of V H and V L domains.
J S, Huston, M N, Margolies, E, Haber
openaire   +2 more sources

Photoactivable Antibody Binding Protein: Site-Selective and Covalent Coupling of Antibody

Analytical Chemistry, 2009
Here we report new photoactivable antibody binding proteins, which site-selectively capture antibodies and form covalent conjugates with captured antibodies upon irradiation. The proteins allow the site-selective tagging and/or immobilization of antibodies with a highly preferred orientation and omit the need for prior antibody modifications.
Yongwon, Jung, Jeong Min, Lee, Jung-won, Kim, Jeongwon, Yoon, Hyunmin, Cho, Bong Hyun, Chung   +5 more
openaire   +3 more sources

Binding site of the rat liver specific monoclonal antibody

Biochemical and Biophysical Research Communications, 1988
A rat liver-specific antigen (RLSA) lost its binding ability to the corresponding monoclonal antibody after treatment with N-glycanase or sialidase, which suggested that the specific binding site might be in a portion of the sugar chain containing sialic acid.
K, Sato, T, Ikeda, Y, Osada
openaire   +2 more sources

Specific Orientation of Porphyrin at the Binding Site of Catalytic Antibody

Biochemical and Biophysical Research Communications, 1996
The catalytic antibody which catalyzes insertion of a cupric ion into mesoporphyrin bound ferric N-methyl mesoporhyrin and ferric mesoporhyrin to yield the respective complexes. The binding affinity of cyanide to the ferric iron in the complexes was compared with that of free ferric porphyrins.
Y, Kawamura-Konishi, S, Neya, N, Funasaki, H, Suzuki   +3 more
openaire   +2 more sources

Mapping antibody binding sites on protein antigens

Nature, 1992
The large surfaces of protein antigens that interact with antibody-combining sites can be determined using deuterium-exchange labelling and two-dimensional 1H nuclear magnetic resonance (NMR). This technique may also be applied to other protein-protein interactions to identify key residues that contribute to the affinity.
openaire   +2 more sources

Proximity of antibody binding sites studied by fluorescence energy transfer

Biochemistry, 1980
Fluorescence energy transfer experiments by steady-state and nanosecond monophoton techniques were carried out with a covalently linked hybrid rabbit IgG antibody containing one antilactose site and one anti-Dns [5-(dimethylamino)-1-naphthalenesulfonyl] site.
R, Luedtke, C S, Owen, F, Karush
openaire   +2 more sources

Number of binding sites of rabbit macroglobulin antibody and its subunits

Immunochemistry, 1965
Abstract The IgM (γ1- or s2-macroglobulin) antibody was purified from rabbit antisera against the p-azobenzenearsonate group. The preparations obtained were shown to be of high purity by ultracentrifugation, radioimmunoelectrophoresis and absorption with a specific immunoadsorbent.
K, Onoue, Y, Yagi, A L, Grossberg, D, Pressman   +3 more
openaire   +2 more sources