Results 271 to 280 of about 8,324,980 (383)

Binding Site Determination from Kinetic Data

, 1971
Edward A. Meighen, J. Woodland Hastings
openalex   +1 more source

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

Essential roles of CTCF binding sites at TAD boundaries in modulating chromatin interactions and transcriptional regulation at the <i>Ifng</i> locus. [PDF]

Front Immunol
Muto K, Yoshida D, Suzuki T, Yamamoto N, Inoue N, Murakami-Sekimata A, Iseki K, Sekimata M.   +7 more
europepmc   +1 more source

Dansylaziridine-labeled troponin C. A fluorescent probe of Ca2+ binding to the Ca2+-specific regulatory sites.

, 1978
J. David Johnson, John H. Collins, John D. Potter   +2 more
openalex   +1 more source

A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms

FEBS Open Bio, EarlyView.
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley   +1 more source

In silico prediction of GRP78-CRIPTO binding sites to improve therapeutic targeting in glioblastoma. [PDF]

Sci Rep
Rashwan ME, Ahmed MR, Elfiky AA.
europepmc   +1 more source

Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position

Nature Methods, 2013
Jason D. Buenrostro, P. Giresi, Lisa C. Zaba, Howard Y. Chang, W. Greenleaf   +4 more
semanticscholar   +1 more source

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

Affinity Electrophoresis of Proteins for Determination of Ligand Affinity and Exploration of Binding Sites. [PDF]

Int J Mol Sci
Masson P, Pashirova T.
europepmc   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source