Results 11 to 20 of about 225,988 (282)

Rapid modeling of experimental molecular kinetics with simple electronic circuits instead of with complex differential equations

Frontiers in Bioengineering and Biotechnology, 2022
Kinetic modeling has relied on using a tedious number of mathematical equations to describe molecular kinetics in interacting reactions. The long list of differential equations with associated abstract variables and parameters inevitably hinders readers’
Yijie Deng, Douglas Raymond Beahm, Xinping Ran, Tanner G. Riley, Rahul Sarpeshkar, Rahul Sarpeshkar   +5 more
doaj   +1 more source

Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution

Bioengineering, 2023
HEK293 is a widely used cell line in the fields of research and industry. It is assumed that these cells are sensitive to hydrodynamic stress. The aim of this research was to use particle image velocimetry validated computational fluid dynamics (CFD) to ...
Stefan Seidel, Rüdiger W. Maschke, Fruhar Mozaffari, Regine Eibl-Schindler, Dieter Eibl   +4 more
doaj   +1 more source

Designing of an extract production protocol for industrial application of cell‐free protein synthesis technology: Building from a current best practice to a quality by design approach

Engineering Biology, 2023
Cell‐Free Protein Synthesis (CFPS) has, over the past decade, seen a substantial increase in interest from both academia and industry. Applications range from fundamental research, through high‐throughput screening to niche manufacture of therapeutic ...
Beatrice Judith Melinek, Jade Tuck, Philip Probert, Harvey Branton, Daniel G. Bracewell   +4 more
doaj   +1 more source

Protein engineering in the computational age: An open source framework for exploring mutational landscapes in silico

Engineering Biology, 2023
The field of protein engineering has seen tremendous expansion in the last decade, with researchers developing novel proteins with specialised functionalities for a range of uses, from drug discovery to industrial biotechnology.
Shirin Bamezai, Giovanni Maresca di Serracapriola, Freya Morris, Rasmus Hildebrandt, Marc Augustine Sojerido Amil, Sporadicate iGEM Team, Rodrigo Ledesma‐Amaro   +6 more
doaj   +1 more source

Biologically engineered microbes for bioremediation of electronic waste: Wayposts, challenges and future directions

Engineering Biology, 2022
In the face of a burgeoning stream of e‐waste globally, e‐waste recycling becomes increasingly imperative, not only to mitigate the environmental and health risks it poses but also as an urban mining strategy for resource recovery of precious metals ...
Ping Han, Wei Zhe Teo, Wen Shan Yew
doaj   +1 more source

A view on chemical and biochemical engineering: Where are they going? [PDF]

Hemijska Industrija, 2002
A short history of chemical and biochemical engineering is presented, both industrial and educational aspects being considered. The most important trend in the future development of bio/chemical engineering - biological engineering - is pointed out.
Veljković Vlada B.
doaj   +1 more source

Type I-F CRISPR-PAIR platform for multi-mode regulation to boost extracellular electron transfer in Shewanella oneidensis

iScience, 2022
Summary: Bio-electrochemical systems are based on extracellular electron transfer (EET), whose efficiency relates to the expression level of numerous genes.
Yaru Chen, Meijie Cheng, Hao Song, Yingxiu Cao   +3 more
doaj   +1 more source

Optical control of the β2-adrenergic receptor with opto-prop-2: A cis-active azobenzene analog of propranolol

iScience, 2022
Summary: In this study, we synthesized and evaluated new photoswitchable ligands for the beta-adrenergic receptors β1-AR and β2-AR, applying an azologization strategy to the first-generation beta-blocker propranolol. The resulting compounds (Opto-prop-1,
Reggie Bosma, Nicola C. Dijon, Yang Zheng, Hannes Schihada, Niels J. Hauwert, Shuang Shi, Marta Arimont, Rick Riemens, Hans Custers, Andrea van de Stolpe, Henry F. Vischer, Maikel Wijtmans, Nicholas D. Holliday, Diederik W.D. Kuster, Rob Leurs   +14 more
doaj   +1 more source

Development of a novel defined minimal medium for Gluconobacter oxydans 621H by systematic investigation of metabolic demands

Journal of Biological Engineering, 2022
Background Historically, complex media are used for the cultivation of Gluconobacter oxydans in industry and research. Using complex media has different drawbacks like higher costs for downstream processing and significant variations in fermentation ...
Svenja Battling, Johannes Pastoors, Alexander Deitert, Tobias Götzen, Lukas Hartmann, Eliot Schröder, Stanislav Yordanov, Jochen Büchs   +7 more
doaj   +1 more source

Serum-free lentiviral vector production is compatible with medium-resident nuclease activity arising from adherent HEK293T host cells engineered with a nuclease-encoding transgene

Heliyon, 2023
At present lentiviral vector production for cell and gene therapy commonly involves transient plasmid transfection of mammalian cells cultivated in serum-containing media and addition of exogenous nuclease to reduce host cell and plasmid DNA impurities ...
Sadfer Ali, Milena Rivera, John Ward, Eli Keshavarz-Moore, Chris Mason, Darren N. Nesbeth   +5 more
doaj   +1 more source