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The field of protein engineering has seen tremendous expansion in the last decade, with researchers developing novel proteins with specialised functionalities for a range of uses, from drug discovery to industrial biotechnology.
Shirin Bamezai+6 more
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Background Historically, complex media are used for the cultivation of Gluconobacter oxydans in industry and research. Using complex media has different drawbacks like higher costs for downstream processing and significant variations in fermentation ...
Svenja Battling+7 more
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Biochemical engineering of 5hmdC-DNA using a Tet3 double-mutant
Sahin H+6 more
europepmc +2 more sources
Energy-based Analysis of Biochemical Cycles using Bond Graphs [PDF]
Thermodynamic aspects of chemical reactions have a long history in the Physical Chemistry literature. In particular, biochemical cycles - the building-blocks of biochemical systems - require a source of energy to function.
Crampin, Edmund J., Gawthrop, Peter J.
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On Projection-Based Model Reduction of Biochemical Networks-- Part II: The Stochastic Case [PDF]
In this paper, we consider the problem of model order reduction of stochastic biochemical networks. In particular, we reduce the order of (the number of equations in) the Linear Noise Approximation of the Chemical Master Equation, which is often used to ...
Anderson, James, Sootla, Aivar
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Utilizing osteocyte derived factors to enhance cell viability and osteogenic matrix deposition within IPN hydrogels [PDF]
Many bone defects arising due to traumatic injury, disease, or surgery are unable to regenerate, requiring intervention. More than four million graft procedures are performed each year to treat these defects making bone the second most commonly ...
Hoey, David A.+2 more
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Water flux is one of the most important performance parameters of the reverse osmosis (RO) membrane. The higher water flux means lower energy cost when treating the same volume of feed solution with membrane separation technology.
Zhongyan Chen+5 more
doaj +1 more source
At present lentiviral vector production for cell and gene therapy commonly involves transient plasmid transfection of mammalian cells cultivated in serum-containing media and addition of exogenous nuclease to reduce host cell and plasmid DNA impurities ...
Sadfer Ali+5 more
doaj +1 more source
An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics [PDF]
The use of protein tagging to facilitate detailed characterization of target proteins has not only revolutionized cell biology, but also enabled biochemical analysis through efficient recovery of the protein complexes wherein the tagged proteins reside ...
De Sutter, Delphine+9 more
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Systems Biology Graphical Notation: Entity Relationship language Level 1 [PDF]
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace.
Anatoly Sorokin+5 more
core +3 more sources