Results 41 to 50 of about 261,713 (289)

Glucose limited feed strategy leads to increased production of fusicocca-2,10(14)-diene by Saccharomyces cerevisiae

AMB Express, 2018
Fusicocca-2,10(14)-diene (FCdiene) is a diterpene which is interesting as a precursor of the anti-cancer drug fusicoccin A and therefore for pharmaceutical applications. Production of FCdiene using a genetically modified Saccharomyces cerevisiae has been
Lisa Marie Halka, Christian Nowacki, Alica Kleinschmidt, Kevin Koenen, Rolf Wichmann   +4 more
doaj   +1 more source

Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates

BMC Biotechnology, 2023
Background One critical parameter in microbial cultivations is the composition of the cultivation medium. Nowadays, the application of chemically defined media increases, due to a more defined and reproducible fermentation performance than in complex ...
Jennifer Goldmanns, Georg Andreas Röhling, Marie Kristine Lipa, Theresa Scholand, Alexander Deitert, Tobias May, Evangeline Priya Haas, Matthias Boy, Andrea Herold, Jochen Büchs   +9 more
doaj   +1 more source

Engineering Cell–ECM–Material Interactions for Musculoskeletal Regeneration

, 2023
The extracellular microenvironment regulates many of the mechanical and biochemical cues that direct musculoskeletal development and are involved in musculoskeletal disease.
Brian T. Penney, Sophia K. Theodossiou, Calvin L. Jones   +2 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter plate

BMC Biotechnology, 2023
Background In industrial microbial biotechnology, fed-batch processes are frequently used to avoid undesirable biological phenomena, such as substrate inhibition or overflow metabolism.
Sarah Sparviero, Laura Barth, Timm Keil, Carl Dinter, Christoph Berg, Clemens Lattermann, Jochen Büchs   +6 more
doaj   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

Microbial Cell Factories, 2019
Background Production of 2,3-butanediol from renewable resources is a promising measure to decrease the consumption of fossil resources in the chemical industry.
Benedikt Heyman, Robin Lamm, Hannah Tulke, Lars Regestein, Jochen Büchs   +4 more
doaj   +1 more source

Benchmarking biopharmaceutical process development and manufacturing cost contributions to R&D

mAbs, 2020
This study aims to benchmark and analyze the process development and manufacturing costs across the biopharmaceutical drug development cycle and their contribution to overall research and development (R&D) costs.
Suzanne S. Farid, Max Baron, Christos Stamatis, Wenhao Nie, Jon Coffman   +4 more
doaj   +1 more source

Harnessing bacterial periplasmic binding proteins as biosensors for on-line process analytical technologies

, 2022
Ligand-binding proteins have good to moderate binding affinity for various small molecules, usually in the millimolar to micromolar range. Many industrially and clinically relevant metabolites in cell culture also fall in these concentration ranges ...
Wong, Lynn
core   +1 more source