Results 31 to 40 of about 261,713 (289)

Spotting priming-active compounds using parsley cell cultures in microtiter plates

BMC Plant Biology, 2023
Background Conventional crop protection has major drawbacks, such as developing pest and pathogen insensitivity to pesticides and low environmental compatibility. Therefore, alternative crop protection strategies are needed. One promising approach treats
Kyra Hoffmann, Jana Viola Schilling, Georg Wandrey, Tim Welters, Stefan Mahr, Uwe Conrath, Jochen Büchs   +6 more
doaj   +1 more source

Improving Haemophilus influenzae Type b Polysaccharide Productivity Through Continuous Culture for Pentavalent Vaccine Manufacturing

Fermentation
Haemophilus influenzae type b (Hib) is a Gram-negative bacterium that causes severe infections in children under five, especially in developing countries.
Lucas Santos Solidade, Lucas Dias Vieira, Mickie Takagi   +2 more
doaj   +1 more source

Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli

Scientific Reports, 2022
Bovine enterokinase light chain (EKL) is an industrially useful protease for accurate removal of affinity-purification tags from high-value biopharmaceuticals.
Weiluo Lee, Subhas Pradhan, Cheng Zhang, Niccolo A. E. Venanzi, Weina Li, Stephen Goldrick, Paul A. Dalby   +6 more
doaj   +1 more source

Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

Microbial Cell Factories, 2018
Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism.
P. Philip, D. Kern, J. Goldmanns, F. Seiler, A. Schulte, T. Habicher, J. Büchs   +6 more
doaj   +1 more source

Highly efficient fermentation of 5-keto-d-fructose with Gluconobacter oxydans at different scales

Microbial Cell Factories, 2022
Background The global market for sweeteners is increasing, and the food industry is constantly looking for new low-caloric sweeteners. The natural sweetener 5-keto-d-fructose is one such candidate.
Svenja Battling, Tobias Engel, Elena Herweg, Paul-Joachim Niehoff, Matthias Pesch, Theresa Scholand, Marie Schöpping, Nina Sonntag, Jochen Büchs   +8 more
doaj   +1 more source

Systematic evaluation of characteristics of the membrane-based fed-batch shake flask

Microbial Cell Factories, 2017
Background The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions.
P. Philip, K. Meier, D. Kern, J. Goldmanns, F. Stockmeier, C. Bähr, J. Büchs   +6 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Application of the Zimm-Bragg Model to the Removal of Azo Dyes with Pectin

Adsorption Science & Technology, 2021
In this work, the ability of pectin (Pec) to remove direct red 80 (DR80), Congo red (CR), methyl orange (MO), and methyl red (MR) was studied. The removal percentages under adequate pH and ionic strength conditions were as follows: DR80 (99.5%), CR (99.8%
Juan Antonio Lozano-Álvarez, Juan Jáuregui-Rincón, Iliana E. Medina-Ramírez, Rogelio Salinas-Gutiérrez, Jorge Martín Alférez Chávez, Guadalupe Javier Araiza-Arvilla   +5 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Chinese herbal Jin-Ying-Tang attenuates the inflammatory response by inhibiting the activation of TLR4/MyD88/TRAF-6/NIK pathway at the mRNA level in LPS-stimulated mouse mammary epithelial cells

Journal of Veterinary Research, 2016
Introduction: The effects of Jin-Ying-Tang (JYT) on Toll-like Receptor 4 (TLR4) signalling transduction of lipopolysaccharide (LPS)-stimulated mouse mammary epithelial cells (MECs) in vitro were examined.
Yi Qiong, Li Xin, Li Yuan-Fang, Yang Hang, Zhang Xiao-Yi, Ma Zhe, Wang Lu   +6 more
doaj   +1 more source