Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations [PDF]
Engineering in Life SciencesFor about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development.
Dominik Engel +2 more
exaly +6 more sources
Predictive Monitoring of Shake Flask Cultures with Online Estimated Growth Models [PDF]
Bioengineering, 2021 Simplicity renders shake flasks ideal for strain selection and substrate optimization in biotechnology. Uncertainty during initial experiments may, however, cause adverse growth conditions and mislead conclusions.
Barbara Pretzner +2 more
exaly +6 more sources
Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress ...
Marcel Schrader +15 more
doaj +5 more sources
Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique [PDF]
Journal of Biological Engineering, 2017 Background Biotechnological development in shake flask necessitates vital engineering parameters e.g. volumetric power input, mixing time, gas liquid mass transfer coefficient, hydromechanical stress and effective shear rate.
Amizon Azizan, Jochen Büchs
doaj +3 more sources
Workflow for shake flask and plate cultivations with fats for polyhydroxyalkanoate bioproduction. [PDF]
Appl Microbiol Biotechnol, 2023 Abstract Since natural resources for the bioproduction of commodity chemicals are scarce, waste animal fats (WAF) are an interesting alternative biogenic residual feedstock. They appear as by-product from meat production, but several challenges are related to their application: first, the high melting points (up to 60 ...
Riedel SL +6 more
europepmc +7 more sources
Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask [PDF]
Microbial Cell Factories, 2018 Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism.
P. Philip +6 more
doaj +3 more sources
Foam Formation in Shake Flasks and Its Consequences [PDF]
Engineering in Life SciencesFoam formation in stirred tank fermentation processes is a well‐studied phenomenon. However, foaming in shake flask cultivations is rarely considered. Non‐baffled shake flasks, in particular, are generally considered to prevent foaming problems. However,
David Vonester +9 more
doaj +6 more sources
International Islamic University Malaysia Engineering Journal, 2011 Chinese hamster ovary (CHO) cells have been most widely used as the production host for the commercial production of biopharmaceuticals product.
S.N.Z Zainul Abidin, and N. Anuar
doaj +3 more sources
Analysis and effect of conventional flasks in shaking culture of Escherichia coli [PDF]
AMB Express, 2020 The circulation direct monitoring and sampling system (CDMSS) is used as a monitoring device for CO2 and O2 concentrations of bypass type in shake-culture flask.
Masato Takahashi, Hideki Aoyagi
doaj +3 more sources
Levan Production in Shake Flask and Fermenter Influence of Feeding Strategy on Levan Yield and Molecular Weight Distribution [PDF]
E3S Web of Conferences, 2023 Effect of feeding strategy on levan production was studied in a shake flask and a 5 L lab scale fermenter. In a shake flask, levan specific substrate yield (YP/S) increased from 0.35 g levan/g sucrose to 0.48 g levan/g sucrose with repeated batch feeding.
Veerapandian Bhuvaneshwari +5 more
doaj +3 more sources