Results 11 to 20 of about 22,410 (289)

Systematic evaluation of characteristics of the membrane-based fed-batch shake flask [PDF]

open access: yesMicrobial Cell Factories, 2017
Background The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions.
P. Philip   +6 more
doaj   +2 more sources

Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases

open access: yesActa Pharmaceutica, 2019
A new method for determination of distribution coefficient of drugs azathioprine, 6-mercaptopurine and 6-thioguanine and nutrient folic acid used in the treatment of inflammatory bowel disease based on a miniaturized shake-flask and HPLC/DAD was ...
Brusač Edvin   +3 more
doaj   +3 more sources

High cell density cultivation of Bacillus subtilis NCIM 2063: Modeling, optimization and a scale-up procedure [PDF]

open access: yesJournal of the Serbian Chemical Society, 2023
Bacillus subtilis is a non-pathogenic, sporulating, Gram-positive bacteria with pronounced antimicrobial and metabolic activity and great potential for wide application in various fields. The aim of this paper was to determine the optimum B.
Stamenković-Stojanović Sandra   +4 more
doaj   +1 more source

Effects of experimental conditions on solubility measurements for BCS classification in order to improve the biowaiver guidelines

open access: yesBrazilian Journal of Pharmaceutical Sciences, 2021
Among the methods described for determining the solubility, shake-flask is suitable to evaluate the equilibrium solubility according to the BCS. Nevertheless, experimental conditions related to the shake-flask method are not well described.
Patrícia Fernanda Monteiro   +5 more
doaj   +1 more source

Effect of Shaking Speed and Type of Closure on Shake Flask Cultures [PDF]

open access: yesApplied Microbiology, 1969
Growth of microorganisms and biosynthesis of microbial products in shake flasks may be limited by operating conditions which provide inadequate supplies of oxygen. Methods are described for meeting the oxygen requirements of test organisms by using standard baffled flasks with pad-type closures and shaking at relatively high speeds. Growth
L E, McDaniel, E G, Bailey
openaire   +2 more sources

Metabolic Transformation and Fermentation Condition of L-homoserine Synthesis by Corynebacterium glutamicum

open access: yesShipin gongye ke-ji, 2023
Objective: In this study, Corynebacterium glutamicum ATCC 13032 was used as the chassis cell for synthesizing L-homoserine and analyzing the effect of dissolved oxygen on product synthesis. Methods: First, the product tolerance of C.
Qiushuang GUO   +6 more
doaj   +1 more source

Bioleaching of chalcopyrite using native Acidithiobacillus ferrooxidans isolated in a mining area in Kitwe Zambia

open access: yesScientific African, 2023
This study aimed to assess the influence of the physical-chemical factors (particle size, shake flask speed, starting pH, and pulp density) in bioleaching of Zambian chalcopyrite using a novel native isolated Acidithiobacillus ferrooxidans strain DJN1 ...
Davis John Nangali   +7 more
doaj   +1 more source

Breeding of a High-Yield Glutathione-Producing Strain by Atmospheric and Room Temperature Plasma Mutagenesis and Microbial Microdroplet Culture System [PDF]

open access: yesShipin Kexue, 2023
In order to improve the ability of a wild-type strain (BY-1) of Pichia pastoris to produce glutathione (GSH), a mutant strain designated BY-1-26 was obtained by atmospheric and room temperature plasma (ARTP) mutagenesis. To further improve the production
LING Siyu, WANG Zhou, ZHANG Huimin, LI Chuang, LIU Qingtao, LIU Yan, XUE Zhenglian
doaj   +1 more source

Enhanced Phenazine-1-Carboxamide Production in Pseudomonas chlororaphis H5△fleQrelA through Fermentation Optimization

open access: yesFermentation, 2022
Phenazine-1-carboxamide (PCN) is effective to control many plant pathogens, and improving PCN production would be of great significance in promoting its development as a biopesticide.
Jiajia Cui   +4 more
doaj   +1 more source

Supplemental Material - SHAKE FLASK DATA - PhD Thesis Lars Barthel

open access: yes, 2023
This dataset contains supplemental material for the PhD thesis of Lars Barthel "Understanding the chitin synthases repertoire of the filamentous fungus Aspergillus niger".
Barthel, Lars
core   +1 more source

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