Results 61 to 70 of about 261,713 (289)

Engineering cellular immunotherapies using lipid-tailed antigen and adjuvant delivery

, 2020
The immune system is the best long-term defense mechanism against prevalent diseases such as cancer. Novel immunotherapies are emerging as frontline treatments for previously incurable diseases by awakening suppressed or reinvigorating dysregulated ...
Zhang, Michael
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Hyperspectral Imaging Techniques for Lyophilization: Advances in Data‐Driven Modeling Strategies and Applications

Advanced Science
Lyophilization, aka freeze drying, is a key process used in the production of biotherapeutic products. The optimization of lyophilization formulations and operations is a slow process that could be accelerated by on‐line analytics.
Huiwen Yu, Prakitr Srisuma, Cedric Devos, Jie Wang, Allan S. Myerson, Richard D. Braatz   +5 more
doaj   +1 more source

Tailoring tumor-recognizable hyaluronic acid–lipid conjugates to enhance anticancer efficacies of surface-engineered natural killer cells

Nano Convergence, 2023
Natural killer (NK) cells have clinical advantages in adoptive cell therapy owing to their inherent anticancer efficacy and their ability to identify and eliminate malignant tumors. However, insufficient cancer-targeting ligands on NK cell surfaces often
Chae Eun Lee, Sungjun Kim, Hee Won Park, Wonjeong Lee, Ashok Kumar Jangid, Yonghyun Choi, Woo-Jin Jeong, Kyobum Kim   +7 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations

Engineering in Life Sciences
For about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development.
Gesa Brauneck, Dominik Engel, Luca Antonia Grebe, Maximilian Hoffmann, Philipp Georg Lichtenberg, Anne Neuß, Marcel Mann, Jorgen Barsett Magnus   +7 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with Corynebacterium glutamicum

Biotechnology for Biofuels, 2019
Background l-Histidine biosynthesis is embedded in an intertwined metabolic network which renders microbial overproduction of this amino acid challenging. This is reflected in the few available examples of histidine producers in literature.
Andreas Schwentner, André Feith, Eugenia Münch, Judith Stiefelmaier, Ira Lauer, Lorenzo Favilli, Christoph Massner, Johannes Öhrlein, Bastian Grund, Andrea Hüser, Ralf Takors, Bastian Blombach   +11 more
doaj   +1 more source

ALTERNATIVE APPROACHES TO INSULIN MANUFACTURING USING CELL-FREE SYSTEMS

, 2023
The modular nature of cell-free protein synthesis (CFPS) has resulted in a paradigm shift in the way scientists can design, discover, and manufacture therapeutic proteins.
Borhani, Shayan
core   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source