Results 81 to 90 of about 3,482,455 (367)

Systems Biology Graphical Notation: Process Description language Level 1 [PDF]

, 2010
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace.
Emek Demir, Falk Schreiber, Huaiyu Mi, Nicolas Le Novere, Stuart L. Moodie   +4 more
core   +4 more sources

Segregationally stabilised plasmids improve production of commodity chemicals in glucose-limited continuous fermentation

Microbial Cell Factories, 2022
Background The production of chemicals via bio-based routes is held back by limited easy-to-use stabilisation systems. A wide range of plasmid stabilisation mechanisms can be found in the literature, however, how these mechanisms effect genetic stability
James R. Allen, Mario A. Torres-Acosta, Naresh Mohan, Gary J. Lye, John M. Ward   +4 more
doaj   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Routes to industrial scalability to maximise investment in engineering biology in the UK—A bioplastics small and medium‐sized enterprise perspective

Engineering Biology
Focus on engineering biology is at the forefront of the UK government's objectives, being one of their five critical technologies (the others being quantum technologies, artifical intelligence, semiconductors, and future telecommunications), with a ...
Amy Switzer, Laima Šusta, Paul Mines
doaj   +1 more source

Serum-free lentiviral vector production is compatible with medium-resident nuclease activity arising from adherent HEK293T host cells engineered with a nuclease-encoding transgene

Heliyon, 2023
At present lentiviral vector production for cell and gene therapy commonly involves transient plasmid transfection of mammalian cells cultivated in serum-containing media and addition of exogenous nuclease to reduce host cell and plasmid DNA impurities ...
Sadfer Ali, Milena Rivera, John Ward, Eli Keshavarz-Moore, Chris Mason, Darren N. Nesbeth   +5 more
doaj  

Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with Corynebacterium glutamicum

Biotechnology for Biofuels, 2019
Background l-Histidine biosynthesis is embedded in an intertwined metabolic network which renders microbial overproduction of this amino acid challenging. This is reflected in the few available examples of histidine producers in literature.
Andreas Schwentner, André Feith, Eugenia Münch, Judith Stiefelmaier, Ira Lauer, Lorenzo Favilli, Christoph Massner, Johannes Öhrlein, Bastian Grund, Andrea Hüser, Ralf Takors, Bastian Blombach   +11 more
doaj   +1 more source

Effect of aprotic solvent on characteristics of Al2O3 ceramic hollow fiber substrates prepared by phase inversion for hydrogen permeation applications

Journal of Asian Ceramic Societies, 2022
The phase inversion procedure was used to prepare Al2O3 ceramic hollow fiber substrates (AlCHFS) utilizing the dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), and 1-methyl-2-pyrrolidone (NMP) aprotic solvents.
Edoardo Magnone, Seung Hwan Lee, Min Chang Shin, Xuelong Zhuang, Jae Yeon Hwang, Jeong In Lee, Jung Hoon Park   +6 more
doaj   +1 more source

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

Inference of nonlinear state-space models for sandwich-type lateral flow immunoassay using extended Kalman filtering [PDF]

, 2011
Copyright [2011] IEEE. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of Brunel University's products or services.
Du, M, Li, Y, Liu, X, Wang, Z, Zeng, N
core   +2 more sources

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source