Results 81 to 90 of about 579,658 (221)

Nanoporous silica-based protocells at multiple scales for designs of life and nanomedicine. [PDF]

, 2015
Various protocell models have been constructed de novo with the bottom-up approach. Here we describe a silica-based protocell composed of a nanoporous amorphous silica core encapsulated within a lipid bilayer built by self-assembly that provides for ...
Brinker, C Jeffrey, Jakobsson, Eric, Sun, Jie, Wang, Yingxiao   +3 more
core   +2 more sources

Unraveling the impact of pH, sodium concentration, and medium osmolality on Vibrio natriegens in batch processes

BMC Biotechnology
Background Vibrio natriegens, a halophilic marine γ-proteobacterium, holds immense biotechnological potential due to its remarkably short generation time of under ten minutes.
Eva Forsten, Steffen Gerdes, René Petri, Jochen Büchs, Jørgen Magnus   +4 more
doaj   +1 more source

An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity

Molecular Therapy: Methods & Clinical Development
We sought to engineer mammalian cells to secrete nuclease activity as a step toward removing the need to purchase commercial nucleases as process additions in bioprocessing of AAV5 and AAV9 as gene therapy vectors. Engineering HeLa cells with a serratial
Geoffrey Howe, Mehtap Bal, Matt Wasmuth, Giulia Massaro, Ahad A. Rahim, Sadfer Ali, Milena Rivera, Desmond M. Schofield, Aminat Omotosho, John Ward, Eli Keshavarz-Moore, Chris Mason, Darren N. Nesbeth   +12 more
doaj   +1 more source

Systems Biology Graphical Notation: Process Diagram Level 1 [PDF]

, 2008
Standard graphical representations have played a crucial role in science and engineering throughout the last century. Without electrical symbolism, it is very likely that our industrial society would not have evolved at the same pace.
Anatoly Sorokin, Emek Demir, Falk Schreiber, Hiroaki Kitano, Huaiyu Mi, Katja Wegner, Michael Hucka, Nicolas Le Novere, Stuart Moodie, Yukiko Matsuoka   +9 more
core   +1 more source

Scale-up of CHO cell cultures: from 96-well-microtiter plates to stirred tank reactors across three orders of magnitude

Journal of Biological Engineering
Background For process development in mammalian cell cultivations, scale-up approaches are essential. A lot of studies concern the scale transfer between different-sized stirred tank reactors.
Anne Neuss, Thomas Steimann, Jacinta Sofia Tomas Borges, Robert Dinger, Jørgen Barsett Magnus   +4 more
doaj   +1 more source

A long-lasting prolactin stimulates galactopoiesis in mice

iScience
Summary: Prolactin is the main hormonal driver of mammalian lactation. To sustain milk production, basal prolactin levels must remain elevated compared to nonpregnant states.
Kasia Kready, Kailyn E. Doiron, Katherine Redfield Chan, Jeffrey C. Way, Quincey Justman, Camille E. Powe, Pamela A. Silver   +6 more
doaj   +1 more source

Molecular Interactions. On the Ambiguity of Ordinary Statements in Biomedical Literature [PDF]

, 2009
Statements about the behavior of biochemical entities (e.g., about the interaction between two proteins) abound in the literature on molecular biology and are increasingly becoming the targets of information extraction and text mining ...
Jansen, Ludger, Schulz, Stefan
core  

Engineering Quorum Quenching Acylases with Improved Kinetic and Biochemical Properties [PDF]

, 2023
Kitty Sompiyachoke, Mikael Elias
openalex   +1 more source

Foam Formation in Shake Flasks and Its Consequences

Engineering in Life Sciences
Foam formation in stirred tank fermentation processes is a well‐studied phenomenon. However, foaming in shake flask cultivations is rarely considered. Non‐baffled shake flasks, in particular, are generally considered to prevent foaming problems. However,
David Vonester, Kyra Hoffmann, Thomas Palmen, Lena Regestein, Ulrike Richter, Anna‐Lena Altenhoff, Maximilian Hoffmann, Yulia Radeva, Jochen Büchs, Jørgen Barsett Magnus   +9 more
doaj   +1 more source

Analysis of Endocrine Signals: The Engineering and Physics of Biochemical Communication Systems1 [PDF]

, 1981
F. Eugene Yates
openalex   +1 more source