Results 91 to 100 of about 261,713 (289)

BioNessie - a grid enabled biochemical networks simulation environment [PDF]

, 2008
The simulation of biochemical networks provides insight and understanding about the underlying biochemical processes and pathways used by cells and organisms.
Gu, Xu, Gilbert, David, Ajayi, Oluwafemi, Ajayi, O., Jiang, Jipu, Gu, X., SINNOTT, RICHARD, Jiang, J., Sinnott, R.O., Liu, X., Gilbert, D., LIU, XUAN   +11 more
core  

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity

Molecular Therapy: Methods & Clinical Development
We sought to engineer mammalian cells to secrete nuclease activity as a step toward removing the need to purchase commercial nucleases as process additions in bioprocessing of AAV5 and AAV9 as gene therapy vectors. Engineering HeLa cells with a serratial
Geoffrey Howe, Mehtap Bal, Matt Wasmuth, Giulia Massaro, Ahad A. Rahim, Sadfer Ali, Milena Rivera, Desmond M. Schofield, Aminat Omotosho, John Ward, Eli Keshavarz-Moore, Chris Mason, Darren N. Nesbeth   +12 more
doaj   +1 more source

Customized microscale approach for optimizing two-phase bio-oxidations of alkanes with high reproducibility

Microbial Cell Factories, 2017
Background Numerous challenges remain to achieve industrially competitive space–time yields for bio-oxidations. The ability to rapidly screen bioconversion reactions for characterization and optimization is of major importance in bioprocess development ...
Johannes F. Kolmar, Oliver Thum, Frank Baganz   +2 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Unraveling the impact of pH, sodium concentration, and medium osmolality on Vibrio natriegens in batch processes

BMC Biotechnology
Background Vibrio natriegens, a halophilic marine γ-proteobacterium, holds immense biotechnological potential due to its remarkably short generation time of under ten minutes.
Eva Forsten, Steffen Gerdes, René Petri, Jochen Büchs, Jørgen Magnus   +4 more
doaj   +1 more source

Biochemical engineering 4 /

, 1986
Bibliogr., indexBased on papers from the fourth international conference on biochemical engineering held on Oct.
International conference on biochemical engineering4thOctober 1-5, 1984Galway, Lim, Henry C.,, Venkatasubramanian, K.,   +2 more
core  

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

FEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source