Results 71 to 80 of about 1,493,364 (293)

exoCasMINI: A T5 exonuclease fused CRISPR-Cas12f system with enhanced gene editing efficiency

iScience
Summary: CRISPR-Cas9 and Cas12a are widely used for genome editing, but their large size limits delivery efficiency. Compact Cas12f proteins offer delivery advantages but suffer from low activity.
Kaitong Liao, Kechen Chen, Shufeng Ma, Xiaofeng Yang, Peihan Chen, Sijie Li, Mengrao Li, Xin Zhang, Ying Lin   +8 more
doaj   +1 more source

Becoming a Scientist: Using First-Year Undergraduate Science Courses to Promote Identification with Science Disciplines [PDF]

, 2016
In this qualitative study, we examined how two professors (a physicist and biochemist) of first year college students perceived their students’ development of identification in biochemistry or physics and how they actively supported this development. The
Jones, Brett D., Ruff, Chloe
core   +4 more sources

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Learning Difficulties and Learning Expected by Students in the Biochemistry Course at Department of Science Education UIN Fatmawati Sukarno Bengkulu

Tadbir: Jurnal Studi Manajemen Pendidikan, 2022
Biochemistry is a science that has a broad scope of material regarding the chemical-biological and chemical-physiological processes of living things.
Qomariah Hasanah, Edi Ansyah
doaj   +1 more source

Critical Role of the Secondary Binding Pocket in Modulating the Enzymatic Activity of DUSP5 toward Phosphorylated ERKs [PDF]

, 2016
DUSP5 is an inducible nuclear dual-specificity phosphatase that specifically interacts with and deactivates extracellular signal-regulated kinases ERK1 and ERK2, which are responsible for cell proliferation, differentiation, and survival. The phosphatase
Bongard, Robert D, Lepley, Michael, Nayak, Jaladhi, Ramchandran, Ramani, Rathore, Rajendra, Sem, Daniel, Talipov, Marat R.   +6 more
core   +3 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

The enzymatic determination of glucose in carbonated beverages: a useful tool for the undergraduate students to learn the basis of enzymatic analysis and the comparison of two analytical methods [PDF]

, 2017
The importance of enzymatic analysis in biochemistry, clinical chemistry and food chemistry is undoubted. The course "Applied Biochemistry" in our Faculty is aimed to undergraduate students of Chemistry and Biochemistry.
Blanco-Lopez, Angel, García-Ponce, Ángel Luis, Martínez-Poveda, Beatriz, Medina, Miguel Ángel, Rodriguez-Quesada, Ana Maria   +4 more
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