Results 1 to 10 of about 169,049 (220)

Enhancement of sorghum biomass hydrolysis by addition of amylases to an ionic liquid-based deconstruction process [PDF]

BMC Research Notes
Objective Conversion of lignocellulosic biomass to renewable biofuels and bio-based chemicals at scale holds great promise for increasing domestic supplies of energy and alternatives to petrochemicals but is contingent upon improvements in biomass ...
Md Tahmid Islam, Venkataramana R. Pidatala, Edward E. K. Baidoo, Paul Wolski, Hemant Choudhary, Daniel H. Putnam, Robert Hutmacher, Jackie Atim, Corinne D. Scown, Henrik V. Scheller, Blake A. Simmons, John M. Gladden, Alberto Rodriguez   +12 more
doaj   +2 more sources

A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities

Scientific Reports, 2021
Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin. Analytical tools capable of quickly detecting both glycan and lignin deconstruction are needed to support the development and characterization of ...
Nicole Ing, Kai Deng, Yan Chen, Martina Aulitto, Jennifer W. Gin, Thanh Le Mai Pham, Christopher J. Petzold, Steve W. Singer, Benjamin Bowen, Kenneth L. Sale, Blake A. Simmons, Anup K. Singh, Paul D. Adams, Trent R. Northen   +13 more
doaj   +1 more source

Expression of a bacterial 3-dehydroshikimate dehydratase (QsuB) reduces lignin and improves biomass saccharification efficiency in switchgrass (Panicum virgatum L.)

BMC Plant Biology, 2021
Background Lignin deposited in plant cell walls negatively affects biomass conversion into advanced bioproducts. There is therefore a strong interest in developing bioenergy crops with reduced lignin content or altered lignin structures.
Zhangying Hao, Sasha Yogiswara, Tong Wei, Veronica Teixeira Benites, Anagh Sinha, George Wang, Edward E. K. Baidoo, Pamela C. Ronald, Henrik V. Scheller, Dominique Loqué, Aymerick Eudes   +10 more
doaj   +1 more source

Biosensor Guided Polyketide Synthases Engineering for Optimization of Domain Exchange Boundaries

Nature Communications, 2023
Type I modular polyketide synthases (PKSs) are multi-domain enzymes functioning like assembly lines. Many engineering attempts have been made for the last three decades to replace, delete and insert new functional domains into PKSs to produce novel ...
Elias Englund, Matthias Schmidt, Alberto A. Nava, Sarah Klass, Leah Keiser, Qingyun Dan, Leonard Katz, Satoshi Yuzawa, Jay D. Keasling   +8 more
doaj   +1 more source

Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor

Biotechnology for Biofuels, 2021
Background The development of bioenergy crops with reduced recalcitrance to enzymatic degradation represents an important challenge to enable the sustainable production of advanced biofuels and bioproducts.
Yang Tian, Chien-Yuan Lin, Joon-Hyun Park, Chuan-Yin Wu, Ramu Kakumanu, Venkataramana R. Pidatala, Khanh M. Vuu, Alberto Rodriguez, Patrick M. Shih, Edward E. K. Baidoo, Stephen Temple, Blake A. Simmons, John M. Gladden, Henrik V. Scheller, Aymerick Eudes   +14 more
doaj   +1 more source

Experimental and theoretical insights into the effects of pH on catalysis of bond-cleavage by the lignin peroxidase isozyme H8 from Phanerochaete chrysosporium

Biotechnology for Biofuels, 2021
Background Lignin peroxidases catalyze a variety of reactions, resulting in cleavage of both β-O-4′ ether bonds and C–C bonds in lignin, both of which are essential for depolymerizing lignin into fragments amendable to biological or chemical upgrading to
Le Thanh Mai Pham, Kai Deng, Trent R. Northen, Steven W. Singer, Paul D. Adams, Blake A. Simmons, Kenneth L. Sale   +6 more
doaj   +1 more source

Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies

BMC Research Notes, 2020
Objectives Sorghum is one of the most recalcitrant species for transformation. Considering the time and effort required for stable transformation in sorghum, establishing a transient system to screen the efficiency and full functionality of vector ...
Rita Sharma, Yan Liang, Mi Yeon Lee, Venkataramana R. Pidatala, Jenny C. Mortimer, Henrik V. Scheller   +5 more
doaj   +1 more source

A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction

Biotechnology for Biofuels, 2019
Background Single guide RNA (sgRNA) selection is important for the efficiency of CRISPR/Cas9-mediated genome editing. However, in plants, the rules governing selection are not well established.
Yan Liang, Aymerick Eudes, Sasha Yogiswara, Beibei Jing, Veronica T. Benites, Reo Yamanaka, Clarabelle Cheng-Yue, Edward E. Baidoo, Jenny C. Mortimer, Henrik V. Scheller, Dominique Loqué   +10 more
doaj   +1 more source

Engineering Pseudomonas putida KT2440 for chain length tailored free fatty acid and oleochemical production

Communications Biology, 2022
Pseudomonas putida has been engineered to produce medium chain length oleochemicals, including medium chain length free fatty acids and their ester forms, compounds which may be useful as biodiesel blending agents.
Luis E. Valencia, Matthew R. Incha, Matthias Schmidt, Allison N. Pearson, Mitchell G. Thompson, Jacob B. Roberts, Marina Mehling, Kevin Yin, Ning Sun, Asun Oka, Patrick M. Shih, Lars M. Blank, John Gladden, Jay D. Keasling   +13 more
doaj   +1 more source

An iron (II) dependent oxygenase performs the last missing step of plant lysine catabolism

Nature Communications, 2020
Hydroxyglutarate synthase (HglS) converts 2-oxoadipate to D-2- hydroxyglutarate during lysine catabolism in bacteria. Here the authors use structural and biochemical approaches to show that HglS acts via successive decarboxylation and intramolecular ...
Mitchell G. Thompson, Jacquelyn M. Blake-Hedges, Jose Henrique Pereira, John A. Hangasky, Michael S. Belcher, William M. Moore, Jesus F. Barajas, Pablo Cruz-Morales, Lorenzo J. Washington, Robert W. Haushalter, Christopher B. Eiben, Yuzhong Liu, Will Skyrud, Veronica T. Benites, Tyler P. Barnum, Edward E. K. Baidoo, Henrik V. Scheller, Michael A. Marletta, Patrick M. Shih, Paul D. Adams, Jay D. Keasling   +20 more
doaj   +1 more source