Results 41 to 50 of about 167,921 (294)

Biotechnology and Bioengineering: Volume 117, Number 11, November 2020

Biotechnology and Bioengineering, 2020
Funding information Kommission für Technologie und Innovation, Grant/Award Number: 19190.2 PFIW‐IW Abstract Integrated continuous manufacturing is entering the biopharmaceutical industry.
L. Pourcel, Flavien Buron, Ghislaine Arib, Valérie Le Fourn, Alexandre Regamey, Iris Bodenmann, Alain Girod, Nicolas Mermod   +7 more
semanticscholar   +1 more source

Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

STAR Protocols
Summary: Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging.
Seungbin Park, Megan Lipton, Yujiao J. Sun, Maria Dadarlat   +3 more
doaj   +1 more source

Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems

STAR Protocols, 2022
Summary: Our recent development of the CRAGE (chassis-independent recombinase-assisted genome engineering) system enables single-step integration of large, complex DNA constructs directly into bacteria genomes across multiple phyla.
Zhiying Zhao, Jan-Fang Cheng, Yasuo Yoshikuni   +2 more
doaj   +1 more source

The Institute for Biotechnology and Bioengineering (IBB) [PDF]

, 2009
The Laboratório Associado Institute for Biotechnology and Bioengineering (IBB) is a research unit aiming to be a strategic infrastructure for the development of the Portuguese R&D and innovation policies in the areas of Biotechnology, Bioengineering ...
Cabral, J. M. S., Ferreira, E. C., Ferreira, Guilherme, Martel, Paulo, Mota, M., Pinto, Henrique Guedes, Reis, R. L.   +6 more
core  

Biotechnology and Bioengineering: Volume 117, Number 5, May 2020

Biotechnology and Bioengineering, 2020
Funding information United Soybean Board, Grant/Award Number: 1840‐352‐0707‐C Abstract It is of great economic interest to produce succinate from low‐grade carbon sources, which can make it more economically competitive against petrochemical‐ based ...
Mohammad H. Ayoubi, Joshaghani, Hassan Dianat, Moghadam, Khaled Seidi, Peyman Zare, Rana Jahanban, Esfahlan   +7 more
semanticscholar   +1 more source

Hematopoietic (stem) cells—The elixir of life?

FEBS Letters, EarlyView.
The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo, Jonah Anderson, Emilie Dinh Vedrenne, Noël Yeh Martín, Jette Lengefeld   +4 more
wiley   +1 more source

Genome editing in Penicillium funiculosum using in vitro assembled CRISPR-Cas9 ribonucleoprotein complexes

STAR Protocols, 2022
Summary: The plasmid-free CRISPR-Cas9-based genome editing in fungi is a precise and time-saving approach. Here, we present a detailed protocol for genetic manipulation in Penicillium funiculosum, which includes design and synthesis of sgRNA, high ...
Tulika Sinha, Syed Shams Yazdani
doaj   +1 more source

Biotechnology and Bioengineering: Volume 117, Number 2, February 2020

Biotechnology and Bioengineering, 2020
Correspondence Mimi C. Yung, Lawrence Livermore National Laboratory, 7000 East Avenue, L‐452, Livermore 94550, CA. Email: yung6@llnl.gov Abstract Antimicrobial peptides (AMPs) are regarded as attractive alternatives to conventional antibiotics, but their
L. Pourcel, Flavien Buron, Ghislaine Arib, Valérie Le Fourn, Alexandre Regamey, Iris Bodenmann, Alain Girod, Nicolas Mermod   +7 more
semanticscholar   +1 more source

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source

High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering

iScience, 2020
Summary: Application of single-stranded DNA recombineering for genome editing of species other than enterobacteria is limited by the efficiency of the recombinase and the action of endogenous mismatch repair (MMR) systems.
Tomas Aparicio, Akos Nyerges, Esteban Martínez-García, Víctor de Lorenzo   +3 more
doaj   +1 more source