Results 51 to 60 of about 102,774 (287)

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Enzymatic Hydrolysis of Cellulosic Materials: A Kinetic Studycarniti

, 1984
Biotechnology and Bioengineering, 26, 1233 (1984)
P. L. Beltrame, A. Marzetti, P. Carniti, V. Sarto, B. Focher   +4 more
core   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Protocol for the development of mRNA lipid nanoparticle vaccines and analysis of immunization efficiency in mice

STAR Protocols
Summary: Here, we present a protocol for the development of mRNA-loaded lipid nanoparticle (LNP) vaccines for target antigen sequences of interest. We describe key steps required to design and synthesize mRNA constructs, their LNP encapsulation, and ...
Neha Karekar, Ashley Reid Cahn, Judit Morla-Folch, Alexis Saffon, Ross W. Ward, Aparna Ananthanarayanan, Abraham J.P. Teunissen, Nina Bhardwaj, Nicolas Vabret   +8 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Protocols for translocation processes of flexible polymers through a pore using LAMMPS

STAR Protocols
Summary: Large-scale Atomic/Molecular Massively Parallel Simulator (LAMMPS) is an open-source, powerful simulator with a customizable platform for extensive Langevin dynamics simulations.
Vrinda Garg, Rejoy Mathew, Arindam Chatterjee, Surya K. Ghosh   +3 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

Protocol for quantification of fungal biomass in fungal composite materials based on ergosterol extraction

STAR Protocols
Summary: Fungal composite materials are lignocellulosic particles and possible inorganic additives, connected by fungal hyphae. Here, we provide a protocol for extracting the cell membrane constituent ergosterol from dried fungal composite material to ...
Carsten Freidank-Pohl, Antonia Geyer, Ulla Simon   +2 more
doaj   +1 more source

Editorial: Extracellular Vesicle Treatment, Epigenetic Modification and Cell Reprogramming to Promote Bone and Cartilage Regeneration

, 2021
10.3389/fbioe.2021.678014FRONTIERS IN BIOENGINEERING AND ...
He, Tong-Chuan, Toh, Wei Seong, Dong, Yufeng, Pei, Ming, Li, Wan-Ju, Pei, Yixuan Amy, Ming Pei, Yufeng Dong, Yixuan Amy Pei, Wan-Ju Li, Tong-Chuan He, Wei Seong Toh   +11 more
core   +1 more source

The heterodimeric amino acid transporters (HAT) of the SLC7/SLC3 family: A structure−function relationships and relevance to human pathology

FEBS Letters, EarlyView.
Heterodimeric amino acid transporters consist of SLC7 and SLC3 family proteins arranged in a conserved structural organization. They regulate nutrient transport across cell membranes, supporting essential cellular functions. These transporters also contribute to xenobiotic/drug uptake and distribution.
Mariafrancesca Scalise, Tiziano Mazza, Raffaella Scanga, Lara Console, Michele Galluccio, Cesare Indiveri   +5 more
wiley   +1 more source