Results 61 to 70 of about 102,774 (287)
STAR ProtocolsSummary: In deep tissue, optogenetics faces limitations with visible light. Here, we present a protocol for near-infrared (NIR) optogenetics manipulation of neurons and motor behavior in Caenorhabditis elegans using emissive upconversion nanoparticles ...
Ruipeng Wang +7 more
doaj +1 more source
, 2020 New and Future Developments in Microbial Biotechnology and Bioengineering: Recent Developments in Trichoderma Research covers topics on-Trichoderma biodiversity, strain improvement and related researches in bioprocess technology, chemical engineering ...
Singh, H. B.,Dr. +3 more
core
Mechatronic design methodology for biotechnology products
, 2009 Biotechnology products can be divided into (1) biologics, which comprise those metabolites, biopolymers, and cell structures that are produced through biological processes, and (2) biotechnical machines, which are apparatuses and devices that transform ...
Mandenius, Carl-Fredrik, +1 more
core +1 more source
, 2021 10.3389/fbioe.2019.00266FRONTIERS IN BIOENGINEERING AND ...
Lu, Wen Feng +5 more
core +1 more source
Neutralization of the PTX3/CD44 axis suppresses TGF‐β1 signaling in kidney fibrosis
FEBS Letters, EarlyView.This study investigates how the PTX3/CD44 axis promotes kidney fibrosis by activating TGF‐β1 signaling. We demonstrate that the neutralizing antibody WHC‐001 effectively inhibits this pathway, suppressing myofibroblast activation and partial epithelial–mesenchymal transition (EMT).
Chao‐Chun Cheng +8 more
wiley +1 more source
DNA ligase LigE increases bacterial transformation with damaged extracellular DNA
FEBS Letters, EarlyView.The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan +4 more
wiley +1 more source
Protocol for large DNA transgenesis in mice using the Cas9+Bxb1 toolbox
STAR ProtocolsSummary: Precise integration of DNA constructs greater than 3 kb into mouse zygotes is difficult. Here, we present a protocol for large DNA transgenesis in mice using the Cas9+Bxb1 toolbox.
Valerie Erhardt +2 more
doaj +1 more source
pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis
, 2021 10.3389/fbioe.2020.00412FRONTIERS IN BIOENGINEERING AND ...
Ding, Jeak Ling +5 more
core +1 more source
Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy
FEBS Letters, EarlyView.The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley +1 more source
Protocol for eVLP-mediated CRISPR-Cas9 and prime editing in pancreatic cancer cell lines
STAR ProtocolsSummary: Pancreatic ductal adenocarcinoma (PDAC) cell lines resist transfection, limiting gene-editing experiments. Here, we present a protocol for delivering CRISPR-Cas9 or prime editors into PDAC cell lines using retrovirus-derived engineered virus ...
Chloe Timmins-Young +6 more
doaj +1 more source