InorganicsRecently, attention has shifted towards the green synthesis of nanoparticles using plant extracts rich in phytochemicals like phenols and flavonoids, offering an alternative method that avoids harmful chemicals and enables large-scale, low-cost ...
Safa Ezzine +3 more
doaj +1 more source
Cry1Ac and Vip3Aa proteins from Bacillus thuringiensis targeting Cry toxin resistance in Diatraea flavipennella and Elasmopalpus lignosellus from sugarcane [PDF]
PeerJ, 2017 The biological potential of Vip and Cry proteins from Bacillus is well known and widely established. Thus, it is important to look for new genes showing different modes of action, selecting those with differentiated entomotoxic activity against Diatraea ...
Ana Rita Nunes Lemes +8 more
doaj +2 more sources
Digital PCR methods improve detection sensitivity and measurement precision of low abundance mtDNA deletions [PDF]
, 2016 Mitochondrial DNA (mtDNA) mutations are a common cause of primary mitochondrial disorders, and have also been implicated in a broad collection of conditions, including aging, neurodegeneration, and cancer.
A Bender +38 more
core +1 more source
Beating the reaction limits of biosensor sensitivity with dynamic tracking of single binding events [PDF]
, 2018 The clinical need for ultra-sensitive molecular analysis has motivated the development of several endpoint assay technologies capable of single molecule readout. These endpoint assays are now primarily limited by the affinity and specificity of the molecular recognition agents for the analyte of interest.
arxiv +1 more source
Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.
Proceedings of the National Academy of Sciences of the United States of America, 1996 Precise mapping of DNA methylation patterns in CpG islands has become essential for understanding diverse biological processes such as the regulation of imprinted genes, X chromosome inactivation, and tumor suppressor gene silencing in human cancer.
J. Herman +4 more
semanticscholar +1 more source
The synthesis of an analogue of the locust CRF-like diuretic peptide, and the biological activities of this and some C-terminal fragments. [PDF]
, 2003 The synthesis is described of an analogue of the locust CRF-like diuretic peptide in which methionine in positions 1,3, and 13 is replaced by isosteric methyl-homoserine residues.
Audsley +19 more
core +1 more source
First-Principles Approach to Electrorotation Assay [PDF]
J. Phys.: Condens. Matter 14, 1213 (2002), 2001 We have presented a theoretical study of electrorotation assay based on the spectral representation theory. We consider unshelled and shelled spheroidal particles as an extension to spherical ones. From the theoretical analysis, we find that the coating can change the characteristic frequency at which the maximum rotational angular velocity occurs. The
arxiv +1 more source
ATR-FTIR spectroscopy detects alterations induced by organotin(IV) carboxylates in MCF-7 cells at sub-cytotoxic/-genotoxic concentrations [PDF]
PMC Biophysics 2008, 1:3, 2009 The environmental impact of metal complexes such as organotin(IV) compounds is of increasing concern. Genotoxic effects of organotin(IV) compounds (0.01 microg/ml, 0.1 microg/ml or 1.0 microg/ml) were measured using the alkaline single-cell gel electrophoresis (comet) assay to measure DNA single-strand breaks (SSBs) and the cytokinesis-block ...
arxiv +1 more source
Durabilidade natural de espécies florestais madeireiras ao ataque de cupim de madeira seca
Floresta e AmbienteEsta pesquisa objetivou avaliar a resistência de dez madeiras ao térmita, Cryptotermes brevis, em condições de laboratório. As espécies estudadas foram amoreira (Chlorophora tinctoria), angelim-pedra (Hymenolobium petraeum), angico-vermelho ...
Fabricio Gomes Gonçalves +4 more
doaj +1 more source
A high throughput molecular force assay for protein-DNA interactions. [PDF]
, 2011 An accurate and genome-wide characterization of protein–DNA interactions such as transcription factor binding is of utmost importance for modern biology. Powerful screening methods emerged.
Albrecht +42 more
core +1 more source