Results 31 to 40 of about 100,345 (222)

GwAAP: A genome-wide amino acid coding-decoding quantitative proteomics system

iScience, 2022
Summary: Mass spectrometry-based proteomic technology has greatly improved and has been widely applied in various biological science fields. However, proteome-wide accurate quantification of proteins in signaling pathways remains challenging.
Li Cheng, Xuetong Yue, Zhaoyu Qin, Xiaogang Sun, Fuchu He, Junbiao Dai, Chen Ding   +6 more
doaj   +1 more source

Long-working-distance high-collection-efficiency three-photon microscopy for in vivo long-term imaging of zebrafish and organoids

iScience
Summary: Zebrafish and organoids, crucial for complex biological studies, necessitate an imaging system with deep tissue penetration, sample protection from environmental interference, and ample operational space.
Peng Deng, Shoupei Liu, Yaoguang Zhao, Xinxin Zhang, Yufei Kong, Linlin Liu, Yujie Xiao, Shasha Yang, Jiahao Hu, Jixiong Su, Ang Xuan, Jinhong Xu, Huijuan Li, Xiaoman Su, Jingchuan Wu, Yuli Jiang, Yu Mu, Zhicheng Shao, Cihang Kong, Bo Li   +19 more
doaj   +1 more source

Sensitive extraction-free SARS-CoV-2 RNA virus detection using a chelating resin

iScience, 2021
Summary: Current conventional detection of SARS-CoV-2 involves collection of a patient’s sample with a nasopharyngeal swab, storage of the swab during transport in a viral transport medium, extraction of RNA, and quantitative reverse transcription PCR ...
Bin Guan, Karen M. Frank, José O. Maldonado, Margaret Beach, Eileen Pelayo, Blake M. Warner, Robert B. Hufnagel   +6 more
doaj   +1 more source

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

FEBS Letters, EarlyView.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source

Unraveling the aging-reversal potency of stem cell-derived extracellular vesicles in a rat model of premature cardiac senescence

iScience
Summary: Cardiosphere-derived cells (CDCs) and their extracellular vesicles (CDC-EVs) can rejuvenate and improve cardiac function, but their efficacy varies among donors. This study aimed to identify predictors of CDC-EV potency.
Lidia Gómez-Cid, Alexia Campo-Fonseca, Mar Cervera-Negueruela, Alejandra Ocampo, Ángel Pinto, Juan Miguel Gil-Jaurena, Susana Suárez-Sancho, Francisco Fernández-Avilés, Lilian Grigorian-Shamagian   +8 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

An avian embryo patient-derived xenograft model for preclinical studies of human breast cancers

iScience, 2021
Summary: Lack of preclinical patient-derived xenograft cancer models in which to conduct large-scale molecular studies seriously impairs the development of effective personalized therapies. We report here an in vivo concept consisting of implanting human
Loraine Jarrosson, Clélia Costechareyre, Fanny Gallix, Séverine Ciré, Fabien Gay, Olivier Imbaud, Romain Teinturier, Elisabetta Marangoni, Karine Aguéra, Céline Delloye-Bourgeois, Valérie Castellani   +10 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia

iScience, 2021
Summary: Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached.
Biyun Zheng, Kyung-Pil Ko, Xuefen Fang, Xiaozhong Wang, Jie Zhang, Sohee Jun, Bong-Jun Kim, Wenyi Luo, Moon Jong Kim, Youn-Sang Jung, Christopher L. Cervantes, Jae-Il Park   +11 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source