Results 31 to 40 of about 311,632 (269)

A light-switching pyrene probe to detect phase-separated biomolecules

open access: yesiScience, 2021
Summary: Biomolecules may undergo liquid–liquid phase separation (LLPS) to spatiotemporally compartmentalize and regulate diverse biological processes. Because the number of tools to directly probe LLPS is limited (ie. FRAP, FRET, fluorescence microscopy,
Masaharu Hazawa   +9 more
doaj   +1 more source

Multiplexed protein profiling reveals spatial subcellular signaling networks

open access: yesiScience, 2022
Summary: Protein-protein interaction networks are altered in multi-gene dysregulations in many disorders. Image-based protein multiplexing sheds light on signaling pathways to dissect cell-to-cell heterogeneity, previously masked by the bulk assays ...
Shuangyi Cai   +7 more
doaj   +1 more source

SkewC: Identifying cells with skewed gene body coverage in single-cell RNA sequencing data

open access: yesiScience, 2022
Summary: The analysis and interpretation of single-cell RNA sequencing (scRNA-seq) experiments are compromised by the presence of poor-quality cells. For meaningful analyses, such poor-quality cells should be excluded as they introduce noise in the data.
Imad Abugessaisa   +9 more
doaj   +1 more source

Are the biomedical sciences ready for synthetic biology?

open access: yesBiomolecular Concepts, 2020
The ability to construct a functional system from its individual components is foundational to understanding how it works. Synthetic biology is a broad field that draws from principles of engineering and computer science to create new biological systems ...
Maxwell S Denies   +2 more
semanticscholar   +1 more source

Specificity and efficiency of tamoxifen-mediated Cre induction is equivalent regardless of age

open access: yesiScience, 2023
Summary: Temporally controlling Cre recombination through tamoxifen (Tam) induction has many advantages for biomedical research. Most studies report early post-natal/juvenile (12 m.o.).
Collyn M. Kellogg   +10 more
doaj   +1 more source

Direct capture and sequencing reveal ultra-short single-stranded DNA in biofluids

open access: yesiScience, 2022
Summary: Cell-free DNA (cfDNA) has become the predominant analyte of liquid biopsy; however, recent studies suggest the presence of subnucleosomal-sized DNA fragments in circulation that are likely single-stranded.
Lauren Y. Cheng   +4 more
doaj   +1 more source

Deciphering the Basis of Molecular Biology of Selected Cardiovascular Diseases: A View on Network Medicine

open access: yesInternational Journal of Molecular Sciences, 2022
Cardiovascular diseases are the leading cause of death across the world. For decades, researchers have been studying the causes of cardiovascular disease, yet many of them remain undiscovered or poorly understood. Network medicine is a recently expanding,
Noora Alhajri   +5 more
semanticscholar   +1 more source

Super-multiplex imaging of cellular dynamics and heterogeneity by integrated stimulated Raman and fluorescence microscopy

open access: yesiScience, 2021
Summary: Observing multiple molecular species simultaneously with high spatiotemporal resolution is crucial for comprehensive understanding of complex, dynamic, and heterogeneous biological systems.
Jingwen Shou   +8 more
doaj   +1 more source

3D visualization of trans-syncytial nanopores provides a pathway for paracellular diffusion across the human placental syncytiotrophoblast

open access: yesiScience, 2022
Summary: The placental syncytiotrophoblast, a syncytium without cell-cell junctions, is the primary barrier between the mother and the fetus. Despite no apparent anatomical pathway for paracellular diffusion of solutes across the syncytiotrophoblast ...
Rohan M. Lewis   +10 more
doaj   +1 more source

Sensitive extraction-free SARS-CoV-2 RNA virus detection using a chelating resin

open access: yesiScience, 2021
Summary: Current conventional detection of SARS-CoV-2 involves collection of a patient’s sample with a nasopharyngeal swab, storage of the swab during transport in a viral transport medium, extraction of RNA, and quantitative reverse transcription PCR ...
Bin Guan   +6 more
doaj   +1 more source

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