Results 41 to 50 of about 430,909 (261)

Specificity and efficiency of tamoxifen-mediated Cre induction is equivalent regardless of age

iScience, 2023
Summary: Temporally controlling Cre recombination through tamoxifen (Tam) induction has many advantages for biomedical research. Most studies report early post-natal/juvenile (12 m.o.).
Collyn M. Kellogg, Kevin Pham, Sunghwan Ko, Jillian E.J. Cox, Adeline H. Machalinski, Michael B. Stout, Amanda L. Sharpe, Michael J. Beckstead, Ana J. Chucair-Elliott, Sarah R. Ocañas, Willard M. Freeman   +10 more
doaj   +1 more source

Efficient sampling methodologies for lake littoral invertebrates in compliance with the European Water Framework Directive [PDF]

, 2015
Lake shores are characterised by a high natural variability, which is increasingly threatened by a multitude of anthropogenic disturbances including morphological alterations to the littoral zone. The European Water Framework Directive (EU WFD) calls for
Brauns, Mario, Donohue, Louise, Jurca, Tamara, Miler, Oliver, Pilotto, Francesca, Porst, Gwendolin, Pusch, Martin, SOLIMINI, ANGELO GIUSEPPE   +7 more
core   +1 more source

Direct capture and sequencing reveal ultra-short single-stranded DNA in biofluids

iScience, 2022
Summary: Cell-free DNA (cfDNA) has become the predominant analyte of liquid biopsy; however, recent studies suggest the presence of subnucleosomal-sized DNA fragments in circulation that are likely single-stranded.
Lauren Y. Cheng, Peng Dai, Lucia R. Wu, Abhijit A. Patel, David Yu Zhang   +4 more
doaj   +1 more source

Post-transcriptional knowledge in pathway analysis increases the accuracy of phenotypes classification [PDF]

, 2016
Motivation: Prediction of phenotypes from high-dimensional data is a crucial task in precision biology and medicine. Many technologies employ genomic biomarkers to characterize phenotypes.
Acunzo, Mario, Alaimo, Salvatore, Ferro, Alfredo, Giugno, Rosalba, Pulvirenti, Alfredo, Veneziano, Dario   +5 more
core   +3 more sources

Super-multiplex imaging of cellular dynamics and heterogeneity by integrated stimulated Raman and fluorescence microscopy

iScience, 2021
Summary: Observing multiple molecular species simultaneously with high spatiotemporal resolution is crucial for comprehensive understanding of complex, dynamic, and heterogeneous biological systems.
Jingwen Shou, Robert Oda, Fanghao Hu, Keiko Karasawa, Mutsuo Nuriya, Masato Yasui, Bruce Shiramizu, Wei Min, Yasuyuki Ozeki   +8 more
doaj   +1 more source

A non-invasive method to genotype cephalopod sex by quantitative PCR

iScience
Summary: Coleoid cephalopods (cuttlefish, octopus, and squid) are emerging model organisms in neuroscience, development, and evolutionary biology and are of major economic importance in global fisheries.
Frederick A. Rubino, Gabrielle C. Coffing, Connor J. Gibbons, Scott T. Small, Thomas Desvignes, Jeffrey Pessutti, Ann M. Petersen, Alexander Arkhipkin, Zhanna Shcherbich, John H. Postlethwait, Andrew D. Kern, Tessa G. Montague   +11 more
doaj   +1 more source

3D visualization of trans-syncytial nanopores provides a pathway for paracellular diffusion across the human placental syncytiotrophoblast

iScience, 2022
Summary: The placental syncytiotrophoblast, a syncytium without cell-cell junctions, is the primary barrier between the mother and the fetus. Despite no apparent anatomical pathway for paracellular diffusion of solutes across the syncytiotrophoblast ...
Rohan M. Lewis, Harikesan Baskaran, Jools Green, Stanimir Tashev, Eleni Palaiologou, Emma M. Lofthouse, Jane K. Cleal, Anton Page, David S. Chatelet, Patricia Goggin, Bram G. Sengers   +10 more
doaj   +1 more source

Mathematical model calibrated to in vitro data predicts mechanisms of antiviral action of the influenza defective interfering particle “OP7”

iScience
Summary: Defective interfering particles (DIPs) are regarded as potent broad-spectrum antivirals. We developed a mathematical model that describes intracellular co-infection dynamics of influenza standard virus (STV) and “OP7”, a new type of influenza ...
Daniel Rüdiger, Julita Piasecka, Jan Küchler, Carolina Pontes, Tanja Laske, Sascha Y. Kupke, Udo Reichl   +6 more
doaj   +1 more source

Environmental and genetic influences on neurocognitive development: the importance of multiple methodologies and time-dependent intervention [PDF]

, 2014
Genetic mutations and environmental factors dynamically influence gene expression and developmental trajectories at the neural, cognitive, and behavioral levels.
Bryson S. E., Clahsen H., D’Souza D., Gopnik M., Guiraud J. A., Karmiloff-Smith A., Pinker S., Wexler K.   +7 more
core   +2 more sources

Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in vitro model of the choroid plexus

iScience, 2022
Summary: The choroid plexus (CP) is a highly vascularized structure containing endothelial and epithelial cells located in the ventricular system of the central nervous system (CNS).
Walter Muranyi, Christian Schwerk, Rosanna Herold, Carolin Stump-Guthier, Marko Lampe, Petra Fallier-Becker, Christel Weiß, Carsten Sticht, Hiroshi Ishikawa, Horst Schroten   +9 more
doaj   +1 more source