Results 221 to 230 of about 1,201,688 (305)

The elephant (bird) in the room: unknown mechanisms and unresolved impacts of low DNA yields in avian microbiome research. [PDF]

Anim Microbiome
Kohl KD, Anderson R, Andreadis C, Baiz MD, Bodawatta KH, Campos-Cerda F, Chaves JA, Clark AB, Costantini MS, Davidson GL, Davies CS, Dietz MW, Florkowski M, Hird SM, Houtz JL, Karasov WH, Knutie SA, Lim HC, Mason NA, Rowe M, Rudzki EN, San Juan PA, Skeen HR, Slevin MC, Somers S, West AG, Worsley SF, Videvall E, Trevelline BK.   +28 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Importance of developmental stage and microenvironment control in Zebrafish larvae cardiovascular studies. [PDF]

PLoS One
Fiorino P, Rosa Cauduro LF, Konig DS, Rosa Cauduro LF, Santos CA, Kavai JA, Duarte ID, Américo ALV.   +7 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Enrique Cerdá-Olmedo: An Outstanding Scientist and Mentor. [PDF]

Microb Biotechnol
Avalos J, Corrochano LM.
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Fast reconstruction of degenerate populations of conductance-based neuron models from spike times. [PDF]

PLoS Comput Biol
Brandoit J, Ernst D, Drion G, Fyon A.
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Correction: Overexpression of cotton PLATZ transcription factors GhPLATZ01 and GhPLATZ15 improves the drought resistance of tomato. [PDF]

BMC Plant Biol
Liu T, Xue Y, Ding X, Zhang Y, Li Z, Zhang Z, Yang Z, Zhu J, Liu R.   +8 more
europepmc   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source