Results 31 to 40 of about 746,277 (164)

Quantitative Comparison of Conventional and t-SNE-guided Gating Analyses

Frontiers in Immunology, 2019
Dimensionality reduction using the t-Distributed Stochastic Neighbor Embedding (t-SNE) algorithm has emerged as a popular tool for visualizing high-parameter single-cell data.
Shadi Toghi Eshghi, Amelia Au-Yeung, Chikara Takahashi, Christopher R. Bolen, Maclean N. Nyachienga, Sean P. Lear, Cherie Green, W. Rodney Mathews, William E. O'Gorman   +8 more
doaj   +1 more source

Nonparametric empirical Bayes biomarker imputation and estimation [PDF]

arXiv, 2023
Biomarkers are often measured in bulk to diagnose patients, monitor patient conditions, and research novel drug pathways. The measurement of these biomarkers often suffers from detection limits that result in missing and untrustworthy measurements. Frequently, missing biomarkers are imputed so that down-stream analysis can be conducted with modern ...
arxiv  

Stable Feature Selection for Biomarker Discovery [PDF]

, 2010
Feature selection techniques have been used as the workhorse in biomarker discovery applications for a long time. Surprisingly, the stability of feature selection with respect to sampling variations has long been under-considered.
He, Zengyou, Yu, Weichuan
core   +2 more sources

Validation of RNAi Silencing Efficiency Using Gene Array Data shows 18.5% Failure Rate across 429 Independent Experiments

Molecular Therapy: Nucleic Acids, 2016
No independent cross-validation of success rate for studies utilizing small interfering RNA (siRNA) for gene silencing has been completed before. To assess the influence of experimental parameters like cell line, transfection technique, validation method,
Gyöngyi Munkácsy, Zsófia Sztupinszki, Péter Herman, Bence Bán, Zsófia Pénzváltó, Nóra Szarvas, Balázs Győrffy   +6 more
doaj   +1 more source

Direct isolation and characterization of circulating exosomes from biological samples using magnetic nanowires

Journal of Nanobiotechnology, 2019
Background Tumor-derived exosomes are gaining attention as important factors that facilitate communication between neighboring cells and manipulate cellular processes associated with cancer development or progression.
Jiyun Lim, Mihye Choi, HyungJae Lee, Young-Ho Kim, Ji-Youn Han, Eun Sook Lee, Youngnam Cho   +6 more
doaj   +1 more source

Analytical evaluation of circulating tumor DNA sequencing assays

Scientific Reports
In China, circulating tumor DNA analysis is widely used and numerous assays are available. Systematic evaluation to help users make informed selections is needed.
Wenjin Li, Xiayu Huang, Rajesh Patel, Erica Schleifman, Shijing Fu, David S. Shames, Jingyu Zhang   +6 more
doaj   +1 more source

Development of a highly sensitive chemiluminescence immunoassay for quantification of aggrecanase-generated ARGS aggrecan fragments in serum

Osteoarthritis and Cartilage Open, 2021
Objective: Cartilage degradation is a hallmark of osteoarthritis (OA). Aggrecan, a major proteoglycan of articular cartilage extracellular matrix (ECM), is degraded by ADAMTS-5 resulting in the release of ARGS-G2 fragments to synovial fluid and ...
Yi He, Kamilla E. Jensen, Anne Sofie Siebuhr, Morten A. Karsdal, Jonathan Larkin, Anne C. Bay-Jensen   +5 more
doaj  

Deep Learning Predicts Biomarker Status and Discovers Related Histomorphology Characteristics for Low-Grade Glioma [PDF]

arXiv, 2023
Biomarker detection is an indispensable part in the diagnosis and treatment of low-grade glioma (LGG). However, current LGG biomarker detection methods rely on expensive and complex molecular genetic testing, for which professionals are required to analyze the results, and intra-rater variability is often reported.
arxiv  

Biomarker and Translational Prostate Cancer Research [PDF]

, 2015
The existing clinical biomarkers for prostate cancer (PCa) are not ideal, since they cannot specifically differentiate between those patients who should be treated immediately and those who should avoid overtreatment.
Clark, Jeremy, Doll, Andreas, Nelson, Colleen   +2 more
core   +4 more sources

Preanalytical approaches to improve recovery of amyloid-β peptides from CSF as measured by immunological or mass spectrometry-based assays

Alzheimer’s Research & Therapy, 2018
Background Amyloid-β 1–42 (Aβ1–42) peptide is a well-established cerebrospinal fluid (CSF) biomarker for Alzheimer’s disease (AD). Reduced levels of Aβ1–42 are indicative of AD, but significant variation in the absolute concentrations of this analyte has
Stephen P. Schauer, William R. Mylott, Moucun Yuan, Rand G. Jenkins, W. Rodney Mathews, Lee A. Honigberg, Kristin R. Wildsmith   +6 more
doaj   +1 more source