Results 41 to 50 of about 244 (211)

Positioning Membrane Proteins by Novel Protein Engineering and Biophysical Approaches [PDF]

Journal of Molecular Biology, 2005
Membrane proteins are unique, in that they can function properly only when they are bound to cellular membranes in a distinct manner. Therefore, positioning of membrane proteins with respect to the membrane is required in addition to the three-dimensional structures in order to understand their detailed molecular mechanisms.
Tatulian, Suren A., Qin, Shan, Pande, Abhay H., He, Xiaomei   +3 more
openaire   +4 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Revealing physical properties of gastric adenocarcinoma cells with two distinct morphologies linking to preferential cellular migration and proliferation

Cell Communication and Signaling
Mechanobiology has played significant roles in guiding and regulating cellular activities, such as morphogenesis, organogenesis, wound healing. Inter-/intracellular force or stiffness as an engine induced cell to reshape their morphologies leading to ...
Dong Wang, Shinji Watanabe, Linhao Sun
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Spatio-Temporal Assessment of Seasonal Variations in Heat Island Intensity in Malayer County Using Remote Sensing Data [PDF]

محیط زیست و توسعه فرابخشی
Introduction: The accelerated pace of urbanization across the globe has resulted in a marked increase in impervious surface coverage, population density, intensified construction activities, and elevated energy consumption.
Elaheh Khangholi, Kamran Shayesteh, Mohammad Reza Gili, Behnaz Attaeian   +3 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Integrated and simultaneous mapping of blue carbon ecosystems by using tide-level, phenological, and biophysical features from optical and SAR images

International Journal of Applied Earth Observations and Geoinformation
Blue carbon ecosystems (BCEs) are nature-based solutions critical for mitigating climate change and biodiversity loss. Accurate mapping of BCEs is fundamental to carbon accounting, maximizing their ecosystem service value, and informing conservation and ...
Leping Wang, Qian Zhang, Yangfan Li
doaj   +1 more source

SENTINEL FOR APPLICATIONS IN AGRICULTURE [PDF]

The International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences, 2019
The European Union and the European Space Agency (EU/ESA) have promoted since 1998 (Baveno Manifesto*) the GMES Programme (Global Monitoring for Environment and Security), nowadays called Copernicus (www.copernicus.eu). In the agriculture domain, the use
M. A. Gomarasca, A. Tornato, D. Spizzichino, E. Valentini, A. Taramelli, A. Taramelli, G. Satalino, M. Vincini, M. Boschetti, R. Colombo, L. Rossi, E. Borgogno Mondino, L. Perotti, W. Alberto, F. Villa   +14 more
doaj   +1 more source

Mapping Spatially Seamless Fractional Vegetation Cover over China at a 30-m Resolution and Semimonthly Intervals in 2010–2020 Based on Google Earth Engine

Journal of Remote Sensing, 2023
Fractional vegetation cover (FVC) is a critical biophysical parameter that characterizes the status of terrestrial ecosystems. The spatial resolutions of most existing FVC products are still at the kilometer level.
Tian Zhao, Xihan Mu, Wanjuan Song, Yaokai Liu, Yun Xie, Bo Zhong, Donghui Xie, Lingmei Jiang, Guangjian Yan   +8 more
doaj   +1 more source

Engineering physical microenvironments to study innate immune cell biophysics

APL Bioengineering, 2022
Innate immunity forms the core of the human body's defense system against infection, injury, and foreign objects. It aims to maintain homeostasis by promoting inflammation and then initiating tissue repair, but it can also lead to disease when dysregulated.
Nikita Kalashnikov, Christopher Moraes
openaire   +3 more sources