Results 91 to 100 of about 39,009 (272)

Scalable Separation and Purification of M13‐Bacteriophage‐Derived Particles Containing Artificial Single‐Stranded DNA Produced by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner, Wiebke Winkler, Maximilian N. Honemann, Hendrik Dietz, Dirk Weuster‐Botz   +4 more
wiley   +1 more source

Artificial Cell Catalysts for Biotransformation: Design Principles and Process Integration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Artificial cell catalysts are emerging as programmable biocatalytic platforms for sustainable molecular conversion. By reconstituting enzymes and functional modules within synthetic compartments, they combine the selectivity of biological catalysis with the design flexibility of synthetic materials.
Iori Kobayashi, Shogo Yoshimoto, Katsutoshi Hori   +2 more
wiley   +1 more source

The potential of microalgae to contribute to sustainable animal feed production in the Arabian Peninsula, Saudi Arabia

Applied Phycology
Microalgal biomass has good potential as a raw material for animal feed, especially fish, being an alternative source of protein and fatty acids. Adopting microalgae for animal feed is a promising solution for addressing food security challenges.
Emna Mhedhbi, Mohamad Padri, Ali Al Shaikhi, Yousef Al Hafedh, Claudio Fuentes Grunewald   +4 more
doaj   +1 more source

Quantitative Partitioning of Photoautotrophic and Photoheterotrophic Contributions During Mixotrophic Growth of Scenedesmus obliquus

Biotechnology and Bioengineering, EarlyView.
Carbon and oxygen fluxes during photoautotrophic, photoheterotrophic and mixotrophic growth of Scenedesmus obliquus. ABSTRACT Mixotrophic growth of microalgae involves the simultaneous assimilation of inorganic and organic carbon sources, but the quantitative contributions of photoautotrophic and photoheterotrophic metabolisms remain poorly resolved ...
Solène Jahan, Thibault Combroux, Samy Kemel, Hélène Marec, Mariana Titica, Howard Fallowfield, Jérémy Pruvost, Guillaume Cogne   +7 more
wiley   +1 more source

Comparison of empirical correlations for the oxygen transfer coefficient estimation in an aerobic bioprocess

Ingenierías USBMed, 2016
This paper shows a comparison of three different empirical correlations found in the literature for the estimation of the oxygen transfer coefficient in an aeration pilot plant using a phenomenological-based semi-physical model (PBSM).
Maribel Ruiz-Botero, Christian Zuluaga-Bedoya, Manuel Ospina-Alarcon, Jose Garcia-Tirado   +3 more
doaj   +1 more source

Unmasking Glycoforms: Lectin‐Based Profiling and Functional Implications of Targeted Glycosylation Knockouts in Chinese Hamster Ovary Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody (mAb) glycosylation is a critical quality attribute that is difficult to rationally engineer and rapidly assess during cell line development. Here, we investigate whether cell‐surface glycosylation can serve as a surrogate readout of mAb product glycosylation following targeted glycogene engineering in CHO cells.
Cristina Abascal Ruiz, Sheryl Li Yan Lim, Jacobus Brink, Sara Carillo, Eoin Casey, Jonathan Bones, Ioscani Jiménez del Val   +6 more
wiley   +1 more source

Aerobic Bioprocess Control Using Artificial Intelligence Techniques

, 2017
This paper deals with the design of an intelligent control structure for a bioprocess of Hansenula polymorpha yeast cultivation. The objective of the process control is to produce biomass in a desired physiological state.
M. Caramihai, Irina Severin
core   +1 more source

Isolating T‐Cell Potent Lentiviral Vector Subpopulations through Rational Design of Nanofiber Anion‐Exchange Adsorbents

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Generation of CAR‐T cell therapies relies predominantly on lentiviral vectors (LVs). As in vivo CAR‐T trials progress, demand for larger quantities of higher‐potency LV is expected. However, primary capture by anion‐exchange chromatography (AIEX) remains a major manufacturing bottleneck from use of adsorbents not tailored to LVs, leading to ...
George Pamenter, Gyorgy Ovari, Anurag Kulkarni, Thomas Williams‐Fegredo, Carol Knevelman, Kyriacos Mitrophanous, Lee Davies, Farzad Foroutan, Matthew Townsend, Stephen Goldrick, Daniel G. Bracewell   +10 more
wiley   +1 more source

Biotechnological Production of Docosahexaenoic Acid Using Aurantiochytrium limacinum: Carbon Sources Comparison And Growth Characterization

Marine Drugs, 2015
Aurantiochytrium limacinum, a marine heterotrophic protist/microalga has shown interesting yields of docosahexaenoic acid (DHA) when cultured with different carbon sources: glucose, pure and crude glycerol.
Sergi Abad, Xavier Turon
doaj   +1 more source

Cell and Gene Therapies Manufacturing Challenges and Integrated Good Manufacturing Practices Solutions: A Lifecycle Perspective

Biotechnology and Bioengineering, EarlyView.
Lifecycle perspective on cell and gene therapy manufacturing challenges and enabling GMP solutions. ABSTRACT Cell and gene therapies (CGTs) are revolutionizing modern medicine; however, making these advanced medicines scalable and readily available to commercial manufacturers worldwide is a major challenge. The number of approved CGT products continues
Rajath Samaga, Udisha Singh, Sachin Dattram Pawar   +2 more
wiley   +1 more source