Results 61 to 70 of about 72,831 (298)

The Ras small GTPase RSR1 regulates cellulase production in Trichoderma reesei

Biotechnology for Biofuels and Bioproducts, 2023
Background Lignocellulose is the most abundant renewable resource in the world and has attracted widespread attention. It can be hydrolyzed into sugars with the help of cellulases and hemicellulases that are secreted by filamentous fungi. Several studies
Ni Li, Zhouyuan Qiu, Wanchuan Cai, Yaling Shen, Dongzhi Wei, Yumeng Chen, Wei Wang   +6 more
doaj   +1 more source

Engineering Anisotropic Mechanical Properties in Large‐Scale Fabricated Cartilage Constructs Using Microfiber Reinforcement

Advanced Healthcare Materials, EarlyView.
This study presents the development of large‐scale (15 cm2) cartilage constructs with anisotropic mechanical properties using melt electrowritten (MEW) microfiber reinforced cell‐laden gelatin methacryloyl (gelMA). These constructs mimic the mechanical behavior of native cartilage, providing a suitable environment for Articular Cartilage ...
Lennard Spauwen, Alba Pueyo Moliner, Patrick van Veenendaal, Roel Custers, Jos Malda, Mylène de Ruijter   +5 more
wiley   +1 more source

CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

Microbial Cell Factories, 2019
Background Pichia pastoris (syn. Komagataella phaffii) is a widely used generally recognized as safe host for heterologous expression of proteins in both industry and academia.
Qi Liu, Xiaona Shi, Lili Song, Haifeng Liu, Xiangshan Zhou, Qiyao Wang, Yuanxing Zhang, Menghao Cai   +7 more
doaj   +1 more source

Mapping molecular pathways for embryonic Sertoli cells derivation based on differentiation model of mouse embryonic stem cells

Stem Cell Research & Therapy, 2020
Background Embryonic Sertoli cells (eSCs) have been known for playing important roles in male reproductive development system. In current studies, eSCs were mainly generated from induced intermediate mesoderm.
Chenze Xu, Yichen Dai, Ali Mohsin, Haifeng Hang, Yingping Zhuang, Meijin Guo   +5 more
doaj   +1 more source

Biofabrication of a Filtration Barrier by Integrating Electrospun Membranes and Flow in a Glomerular Co‐Culture

Advanced Healthcare Materials, EarlyView.
An ex vivo glomerular filtration barrier is engineered using electrospun, biomimetic membranes seeded with human glomerular cells. The model supports fenestrated endothelium and podocyte foot processes under flow, mimics size‐selective filtration, and enables disease modeling with hiPSC‐derived podocytes, offering a powerful platform for studying ...
Camilla Mussoni, Anna Rederer, Vladimir Stepanenko, Frank Würthner, Philipp Stahlhut, Jürgen Groll, Mario Schiffer, Taufiq Ahmad, Janina Müller‐Deile   +8 more
wiley   +1 more source

Microtechnology in Space Bioreactors

CHIMIA, 1999
Space biology is a young and rapidly developing discipline comprising basic research and biotechnology. In the next decades it will play a prominent role in the International Space Station (ISS). Therefore, there is an increasing demand for sophisticated instrumentation to satisfy the requirements of the future projects in space biology.
Isabelle Walther, Bart van der Schoot, Marc Boillat, Otfried Müller, Augusto Cogoli   +4 more
openaire   +4 more sources

Biphasic Granular Bioinks for Biofabrication of High Cell Density Constructs for Dermal Regeneration

Advanced Healthcare Materials, EarlyView.
This study introduces a biphasic granular bioink (µInk) that enables 3D bioprinting of high cell density constructs for dermal regeneration. The bioink promotes cell proliferation, ECM deposition, and neovascularization, leading to tissue integration and reduced scar formation in vivo.
Rozalin Shamasha, Sneha Kollenchery Ramanathan, Kristin Oskarsdotter, Fatemeh Rasti Boroojeni, Aleksandra Zielińska, Sajjad Naeimipour, Philip Lifwergren, Nina Reustle, Lauren Roberts, Annika Starkenberg, Gunnar Kratz, Peter Apelgren, Karin Säljö, Jonathan Rakar, Lars Kölby, Daniel Aili, Johan Junker   +16 more
wiley   +1 more source

Enhancement of cellulase production in Trichoderma reesei RUT-C30 by comparative genomic screening

Microbial Cell Factories, 2019
Background Cellulolytic enzymes produced by the filamentous fungus Trichoderma reesei are commonly used in biomass conversion. The high cost of cellulase is still a significant challenge to commercial biofuel production. Improving cellulase production in
Pei Liu, Aibo Lin, Guoxiu Zhang, Jiajia Zhang, Yumeng Chen, Tao Shen, Jian Zhao, Dongzhi Wei, Wei Wang   +8 more
doaj   +1 more source

Differential Phase Contrast Imaging to Predict MSC Immune Function

Advanced Healthcare Materials, EarlyView.
This work employs quantitative differential phase contrast (qDPC) imaging technique to non‐invasively extract morphological features from live Mesenchymal Stromal Cells (MSCs) for single cell prediction on cell immunomodulatory capacity. This work establishes a foundation for scalable, non‐destructive monitoring of MSCs immunomodulatory capacity ...
Kejie Rui, Priyanka Priyadarshani, Adrian Ross Liversage, Carlos Munoz, Steven Stice, Luke J. Mortensen   +5 more
wiley   +1 more source

An Organ‐on‐Chip Platform for Strain‐Controlled, Tissue‐Specific Compression of Cartilage and Mineralized Osteochondral Interface to Study Mechanical Overloading in Osteoarthritis

Advanced Healthcare Materials, EarlyView.
A mechanically active OsteoChondral Unit (OCU)‐on‐Chip platform mimicking the OCU's functional anatomy and the strain gradient across the osteochondral interface is presented. Upon compartment‐specific hyperphysiological compression, the model replicates mechanisms observed in osteoarthritis (OA) progression, such as calcium crystal accumulation ...
Andrea Mainardi, Anastasiya Börsch, Paola Occhetta, Robert Ivanek, Martin Ehrbar, Lisa Krattiger, Philipp Oertle, Marko Loparic, Ivan Martin, Marco Rasponi, Andrea Barbero   +10 more
wiley   +1 more source