Results 71 to 80 of about 96,256 (296)

Innate Immunocompetent hiPSC‐Derived Neurospheroids Capture Early CNS Responses to rAAV

Advanced Science, EarlyView.
Knowledge of human CNS immune responses to AAV‐based gene therapies remains limited due to the lack of immune‐competent human models. Here, a hiPSC‐derived 3D neuroimmune platform integrating neurospheroids and microglia is established using stirred‐tank bioreactors.
Catarina M. Gomes, Gabriela Silva, Mafalda Aleixo, Marta Gomes, Daniel Simão, Stephan J. Holtkamp, Diana D. Lobo, Pradeep Harish, Rosalind Jenkins, Lekh N. Dahal, Rui J. Nobre, Luís de Pereira de Almeida, Mark Trautwein, Paula M. Alves, Catarina Brito   +14 more
wiley   +1 more source

Transcriptional regulatory networks of methanol-independent protein expression in Pichia pastoris under the AOX1 promoter with trans-acting elements engineering

Bioresources and Bioprocessing, 2020
To explore the differences in the intracellular transcriptional mechanism in carbon-derepressed and wild-type Pichia pastoris strains fed with three different carbon sources.
Lei Shi, Jinjia Wang, Xiaolong Wang, Yuanxing Zhang, Zhiwei Song, Menghao Cai, Xiangshan Zhou   +6 more
doaj   +1 more source

Engineering Approaches to Modify Immunomodulatory Functions of Mesenchymal Stromal Cells (MSCs): Tissue Regeneration and Clinical Application

Advanced Science, EarlyView.
Mesenchymal stromal cells (MSCs) show promise for treating immune‐related disorders through immunomodulation and tissue regeneration. This review gives a brief overview of current clinical approval of MSC therapies. It also discussed how bioengineering, including genetic modification, biomaterial delivery, extracellular vesicles, and iPSC‐derived MSCs,
Sichen Yang, Kejia Li, Ning Wang, Zhen Li, Zhiyong Zhang, Rocky S. Tuan, Yangzi Jiang   +6 more
wiley   +1 more source

Development of an economical fermentation platform for enhanced ansamitocin P-3 production in Actinosynnema pretiosum

Bioresources and Bioprocessing, 2019
Background Ansamitocin P-3 (AP-3), produced by Actinosynnema pretiosum, has extraordinary antitumor activity and has been used as toxic “warhead” in antibody-conjugated drugs. However, AP-3 production is limited by its low yield and thus the high cost of
Ting Liu, Lyailya Bessembayeva, Jun Chen, Liu-Jing Wei, Qiang Hua   +4 more
doaj   +1 more source

Fungal statin pump protein improves monacolin J efflux and regulates its production in Komagataella phaffii

Bioresources and Bioprocessing, 2020
Background Monacolin J (MJ) is a key intermediate for the synthesis of cholesterol-lowering drug simvastatin. Current industrial production of MJ involves complicated chemical hydrolysis of microbial fermented lovastatin.
Chenxiao Bai, Yiqi Liu, Xinjie Chen, Zhilan Qian, Haifeng Liu, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai   +7 more
doaj   +1 more source

Polychip‐A High‐Throughput Droplet Microfluidics Platform for Interrogating Microbial Interactions

Advanced Science, EarlyView.
Polychip, a fully integrated droplet microfluidics platform, enables high‐throughput, single‐cell resolution screening of polymicrobial interactions. By seamlessly combining six automated microfluidics operations on a single chip, the system accelerates antimicrobial discovery by 11 to 14 times compared to traditional robotic methods.
Jeong Jae Han, Adrian Ryan Guzman, Aifen Zhou, Han Zhang, Rohit Gupte, Haemin Jung, Kaylee Delgado, Sini Skariah, Ali Sultan, Arul Jayaraman, Paul de Figueiredo, Arum Han   +11 more
wiley   +1 more source

kLa MEASUREMENT IN BIOREACTORS

Chemical Engineering Transactions, 2010
Abstract preview not available - see full-text PDF article.
Scargiali F., BUSCIGLIO, ANTONIO, Grisafi F., Brucato A.   +3 more
openaire   +2 more sources

Organoid Brain‐Machine‐Interface Devices for Central Nervous System Repair

Advanced Science, EarlyView.
We envision organoid brain‐machine‐interface (Organoid‐BMI) devices as new biohybrid bidirectional communication pathways to connect the human CNS and the external world for personalized CNS repair and regeneration. ABSTRACT Central nervous system (CNS) repair and regeneration suffer from tremendous clinical challenges due to current limitations in ...
Yantao Xing, Yang Yang, Zichen Hong, Chunhui Tian, Huiyu Chu, Susanne Clara Prokop, Hongwei Cai, Mingxia Gu, Jason Tchieu, Ken Mackie, Feng Guo   +10 more
wiley   +1 more source

Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity

Bioresources and Bioprocessing, 2019
Background A rational and high-performance high-throughput screening system effectively improves the efficiency of strain screening. Results A rapid and efficient method was developed to detect carbon sources.
Yumeng Lin, Yang Chen, Qianhui Li, Xiwei Tian, Ju Chu   +4 more
doaj   +1 more source

Multiscale Modelling of the Electrocatalysis of Microenzymes: Insights Into the Performance of Microperoxidase at an Aqueous/Graphene Interface and Their Exploitation to Improve Performance

Advanced Science, EarlyView.
A novel multiscale MD/DFT framework to elucidate microenzyme adsorption and electron transfer is reported. This method reveals distinct configurations for microperoxidase‐11 on graphene and those best suited for electron transfer. Rate constants align with the limited experimental data, establishing this framework as a tool for optimizing microenzyme ...
Milan Mijajlovic, Cheng Hu, Yuhui Sun, Matthew J. Penna, Meisam V. Kiamahalleh, Wenrong Yang, Mark J. Biggs   +6 more
wiley   +1 more source