Results 61 to 70 of about 81,744 (305)

Uridine modulates monoclonal antibody charge heterogeneity in Chinese hamster ovary cell fed-batch cultures

Bioresources and Bioprocessing, 2018
Background Charge heterogeneity is one of the most critical quality attributes of antibodies, which has strong influence on drug’s biological activity and safety.
Huijie Niu, Jiaqi Wang, Mengjuan Liu, Miaomiao Chai, Liang Zhao, Xuping Liu, Li Fan, Wen-Song Tan   +7 more
doaj   +1 more source

Integrating Vibrio natriegens for Photon Manipulation in Living Lighting Devices

Advanced Materials, EarlyView.
Rationalization of genetic and material engineering tools has enabled the engineering of fluorescent Vibrio natriegens (V. natriegens) to fabricate straightforward, highly emissive and stable V. natriegens‐silicones color filters for the first red‐emitting bacteria‐hybrid light‐emitting diodes. This pinpoints V. natriegens as a high‐performance protein
Stephanie Willeit, Maurice Hädrich, Philippe‐Quentin Liss, Valeria Rodriguez Espinoza, Nicklas Maximilian Foerster, Bastian Blombach, Rubén D. Costa   +6 more
wiley   +1 more source

Thiolated Polymers in 3D Bioprinting: Control of Gelation

Advanced Materials, EarlyView.
Thiolated polymers are established as programmable bioinks for 3D bioprinting, integrating versatile crosslinking chemistries with redox‐responsive control. This work demonstrates how molecular design and external triggers define gelation kinetics, printability windows, and structural fidelity, enabling stable, high‐resolution constructs and advancing ...
Soheil Haddadzadegan, Flavia Laffleur, Andreas Bernkop‐Schnürch   +2 more
wiley   +1 more source

Three new LmbU targets outside lmb cluster inhibit lincomycin biosynthesis in Streptomyces lincolnensis

Microbial Cell Factories
Background Antibiotics biosynthesis is usually regulated by the cluster-situated regulatory gene(s) (CSRG(s)), which directly regulate the genes within the corresponding biosynthetic gene cluster (BGC).
Yue Mao, Xianyan Zhang, Tianyu Zhou, Bingbing Hou, Jiang Ye, Haizhen Wu, Ruida Wang, Huizhan Zhang   +7 more
doaj   +1 more source

The Ras small GTPase RSR1 regulates cellulase production in Trichoderma reesei

Biotechnology for Biofuels and Bioproducts, 2023
Background Lignocellulose is the most abundant renewable resource in the world and has attracted widespread attention. It can be hydrolyzed into sugars with the help of cellulases and hemicellulases that are secreted by filamentous fungi. Several studies
Ni Li, Zhouyuan Qiu, Wanchuan Cai, Yaling Shen, Dongzhi Wei, Yumeng Chen, Wei Wang   +6 more
doaj   +1 more source

Cell Adhesion by Design: Engineering Tissue Culture Scaffolds With Adhesion Cues

Advanced Materials Interfaces, EarlyView.
ABSTRACT In scaffold‐based tissue engineering, the matrix should provide adequate adhesion cues for cell attachment, spreading, and function. Given the multitude of adhesion receptors and the diversity of scaffolds, there are many approaches to render scaffolds adhesive, even though they are not all equivalent.
Dalia Dranseike, Dhananjay V. Deshmukh, Mark W. Tibbitt, Céline Labouesse   +3 more
wiley   +1 more source

Transcriptional regulatory networks of methanol-independent protein expression in Pichia pastoris under the AOX1 promoter with trans-acting elements engineering

Bioresources and Bioprocessing, 2020
To explore the differences in the intracellular transcriptional mechanism in carbon-derepressed and wild-type Pichia pastoris strains fed with three different carbon sources.
Lei Shi, Jinjia Wang, Xiaolong Wang, Yuanxing Zhang, Zhiwei Song, Menghao Cai, Xiangshan Zhou   +6 more
doaj   +1 more source

Mechanism of Zn2+ regulation of cellulase production in Trichoderma reesei Rut-C30

Biotechnology for Biofuels and Bioproducts, 2023
Background Trichoderma reesei Rut-C30 is a hypercellulolytic mutant strain that degrades abundant sources of lignocellulosic plant biomass, yielding renewable biofuels.
Ni Li, Jing Li, Yumeng Chen, Yaling Shen, Dongzhi Wei, Wei Wang   +5 more
doaj   +1 more source

A Reproducible and Scalable Process for High Yield Mesenchymal Stromal Cell Extracellular Vesicles Production

Advanced Materials Technologies, EarlyView.
Mesenchymal stem cell derived extracellular vesicles (MSC‐EVs) are a promising therapeutic tool for regenerative medicine. However, the field lacks a reproducible high‐yield production method to answer the quantities needed for clinical translation.
Christophe Wong, Elise Madec, Jeanne Volatron, Max Piffoux, Amanda K. A. Silva, Florence Gazeau, Jean‐Philippe Herbeuval, Thibaut Fourniols   +7 more
wiley   +1 more source

Cooperation of FGF/MEK/ERK and Wnt/β-catenin pathway regulators to promote the proliferation and pluripotency of mouse embryonic stem cells in serum- and feeder-free conditions

Bioresources and Bioprocessing, 2019
The FGF/MEK/ERK and Wnt/β-catenin signaling pathways have previously been proved to regulate mouse embryonic stem cell (mESCs) function. However, the relationships between these two pathways, especially their different functions on proliferation and ...
Tong Zhang, Huanyun Chen, Yiran Zhou, Wanghong Dong, Haibo Cai, Wen-Song Tan   +5 more
doaj   +1 more source