Results 41 to 50 of about 44,737 (248)

Dynamic Mechanical Loading Modulates Extracellular Vesicle Production and Cargo in Meniscus Fibrochondrocytes

Advanced Materials Interfaces, EarlyView.
Dynamic mechanical loading of outer meniscus fibrochondrocytes increases extracellular vesicle (EV) yield and alters EV composition. When applied to progenitor cells, these mechanically primed EVs modulate the expression of matrix‐related genes.
Catherine Cheung, Zizhao Li, Sung‐Han Jo, Se‐Hwan Lee, Il Keun Kwon, Jina Ko, Su Chin Heo   +6 more
wiley   +1 more source

Uridine modulates monoclonal antibody charge heterogeneity in Chinese hamster ovary cell fed-batch cultures

Bioresources and Bioprocessing, 2018
Background Charge heterogeneity is one of the most critical quality attributes of antibodies, which has strong influence on drug’s biological activity and safety.
Huijie Niu, Jiaqi Wang, Mengjuan Liu, Miaomiao Chai, Liang Zhao, Xuping Liu, Li Fan, Wen-Song Tan   +7 more
doaj   +1 more source

Additive‐Manufactured, Multifunctional Bioreactor Technology for Dynamic Culture of 3D Bioprinted Tissue Models

Advanced Materials Technologies, EarlyView.
We introduce an additively manufactured bioreactor with a perfusion flow system and integrated temperature and pH sensors for skeletal muscle tissue biofabrication. The bioreactor's performance was evaluated by assessing the viability, spreading of the myoblast cells in a printed scaffold, and contraction of the isolated murine musculi interossei ...
Lys Sprenger, Johann Schorzmann, Hsuan‐Heng Lu, Muhammad Osama, Julian Bauer, Michael Haug, Oliver Friedrich, Aldo R. Boccaccini, Frank Döpper, Sahar Salehi   +9 more
wiley   +1 more source

Cooperation of FGF/MEK/ERK and Wnt/β-catenin pathway regulators to promote the proliferation and pluripotency of mouse embryonic stem cells in serum- and feeder-free conditions

Bioresources and Bioprocessing, 2019
The FGF/MEK/ERK and Wnt/β-catenin signaling pathways have previously been proved to regulate mouse embryonic stem cell (mESCs) function. However, the relationships between these two pathways, especially their different functions on proliferation and ...
Tong Zhang, Huanyun Chen, Yiran Zhou, Wanghong Dong, Haibo Cai, Wen-Song Tan   +5 more
doaj   +1 more source

The Ras small GTPase RSR1 regulates cellulase production in Trichoderma reesei

Biotechnology for Biofuels and Bioproducts, 2023
Background Lignocellulose is the most abundant renewable resource in the world and has attracted widespread attention. It can be hydrolyzed into sugars with the help of cellulases and hemicellulases that are secreted by filamentous fungi. Several studies
Ni Li, Zhouyuan Qiu, Wanchuan Cai, Yaling Shen, Dongzhi Wei, Yumeng Chen, Wei Wang   +6 more
doaj   +1 more source

Extrusion Bioprinting for Wound Healing: Innovations in Functionalized Bioinks and Bioprinting Technology

Advanced Materials Technologies, EarlyView.
Extrusion‐based bioprinting (EBB) has emerged as a versatile biofabrication platform capable of precisely depositing bioinks composed of biomaterials, cells, and bioactive agents to generate patient‐specific, biomimetic skin constructs. This paper presents a state‐of‐the‐art and forward‐looking overview of EBB for wound healing, encompassing printing ...
Hien‐Phuong Le, Anh Tuan Trong Tran, Ammara Waheed, Tran Thanh Tung, Dusan Losic   +4 more
wiley   +1 more source

Three new LmbU targets outside lmb cluster inhibit lincomycin biosynthesis in Streptomyces lincolnensis

Microbial Cell Factories
Background Antibiotics biosynthesis is usually regulated by the cluster-situated regulatory gene(s) (CSRG(s)), which directly regulate the genes within the corresponding biosynthetic gene cluster (BGC).
Yue Mao, Xianyan Zhang, Tianyu Zhou, Bingbing Hou, Jiang Ye, Haizhen Wu, Ruida Wang, Huizhan Zhang   +7 more
doaj   +1 more source

From Lab to Landscape: Environmental Biohybrid Robotics for Ecological Futures

Advanced Robotics Research, EarlyView.
This Perspective explores environmental biohybrid robotics, integrating living tissues, microorganisms, and insects for operation in real‐world ecosystems. It traces the leap from laboratory experiments to forests, wetlands, and urban environments and discusses key challenges, development pathways, and opportunities for ecological monitoring and ...
Miriam Filippi
wiley   +1 more source

Transcriptional regulatory networks of methanol-independent protein expression in Pichia pastoris under the AOX1 promoter with trans-acting elements engineering

Bioresources and Bioprocessing, 2020
To explore the differences in the intracellular transcriptional mechanism in carbon-derepressed and wild-type Pichia pastoris strains fed with three different carbon sources.
Lei Shi, Jinjia Wang, Xiaolong Wang, Yuanxing Zhang, Zhiwei Song, Menghao Cai, Xiangshan Zhou   +6 more
doaj   +1 more source

Cross Kingdom Metabolic Engineering Paradigm Elevating Sustainable Protein Production

Advanced Science, EarlyView.
ABSTRACT Confronting the dual crisis of escalating global protein demand and unsustainable agriculture necessitates transformative solutions. Here, we pioneer evolutionary insights from maize nitrogen optimization via asparagine synthetase (ASNS) to rewire metabolism in Pichia pastoris.
Yuanyuan Du, Changyu Pi, Kai Hong, Le Gao, Xin Wu   +4 more
wiley   +1 more source