Results 71 to 80 of about 71,391 (320)

Hepatocyte Aggregates: Methods of Preparation in the Microgravity Simulating Bioreactor Use in Tissue Engineering [PDF]

open access: yesarXiv, 2008
Tissue Engineering concerns the three-dimensional cell growth so that bio-artificial tissues could be created and used for transplantation. The recently expressed concerns from the Tissue Engineering research community for a re-direction of the research activities necessitate the proposition of new methodologies. We propose a methodology that has to do
arxiv  

Data-driven and Physics Informed Modelling of Chinese Hamster Ovary Cell Bioreactors [PDF]

open access: yesarXiv, 2023
Fed-batch culture is an established operation mode for the production of biologics using mammalian cell cultures. Quantitative modeling integrates both kinetics for some key reaction steps and optimization-driven metabolic flux allocation, using flux balance analysis; this is known to lead to certain mathematical inconsistencies.
arxiv  

Developing Quorum Sensing‐Based Collaborative Dynamic Control System in Halomonas TD01

open access: yesAdvanced Science, EarlyView.
A standardized and streamlined inducer‐free quorum sensing (QS)‐based collaborative dynamic control pattern is developed for sufficient gene expression control in Halomonas TD by mixing two engineered cells, cells A and B. Proof‐of‐concept studies are designed to test the dynamic control performance in mixed cultures in fed‐batch conditions.
Yi‐Na Lin   +12 more
wiley   +1 more source

Comprehensive reconstruction and evaluation of Pichia pastoris genome-scale metabolic model that accounts for 1243 ORFs

open access: yesBioresources and Bioprocessing, 2017
Background Pichia pastoris is one of the most important cell factories for production of industrial enzymes and heterogenous proteins. The genome-scale metabolic model of high quality is crucial for comprehensive understanding of the P.
Rui Ye   +7 more
doaj   +1 more source

High‐Viability Circulating Tumor Cells Sorting From Whole Blood at Single Cell Level Using Laser‐Induced Forward Transfer‐Assisted Microfiltration

open access: yesAdvanced Science, EarlyView.
A laser‐induced forward transfer‐assisted microfiltration system is developed for single rare cell sorting from undiluted whole blood. The isolated cell has a high viability, enabling successful ex vivo cell culture and efficient single‐cell RNA sequencing.
Qingmei Xu   +16 more
wiley   +1 more source

Towards green biomanufacturing of high-value recombinant proteins using promising cell factory: Chlamydomonas reinhardtii chloroplast

open access: yesBioresources and Bioprocessing, 2022
Microalgae are cosmopolitan organisms in nature with short life cycles, playing a tremendous role in reducing the pressure of industrial carbon emissions.
Ke Ma, Lei Deng, Haizhen Wu, Jianhua Fan
doaj   +1 more source

Reduced Order Dead-Beat Observers for a Bioreactor [PDF]

open access: yesarXiv, 2010
This paper studies the strong observability property and the reduced-order dead-beat observer design problem for a continuous bioreactor. New relationships between coexistence and strong observability, and checkable sufficient conditions for strong observability, are established for a chemostat with two competing microbial species.
arxiv  

Outermost Cationic Surface Charge of Layer‐by‐Layer Films Prevents Endothelial Cells Migration for Cell Compartmentalization in Three‐Dimensional Tissues

open access: yesAdvanced Science, EarlyView.
A nanometer‐sized artificial basement membrane (A‐BM) is developed to replicate the barrier functions of the basement membrane and prevent cell migration. It is demonstrated that the outermost cationic surface charge of nanofilms can effectively inhibit endothelial cell migration and sprouting, allowing for the construction of 3D patterned vascular ...
Jinfeng Zeng   +5 more
wiley   +1 more source

The Encapsulation Strategies for Targeted Delivery of Probiotics in Preventing and Treating Colorectal Cancer: A Review

open access: yesAdvanced Science, EarlyView.
This work summarizes the efficacy and mechanisms of probiotics in CRC treatment, providing a comprehensive overview of various encapsulation methods such as microcapsules, hydrogels, single‐cell nanocoatings, and nanofibers for targeted probiotic delivery to inflammatory sites, with particular emphasis on the potential advantages of emerging single ...
Hao Zhong   +5 more
wiley   +1 more source

Improved methanol-derived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux in methylotrophic yeast

open access: yesBioresources and Bioprocessing, 2018
Background Methanol has attracted interest as a substrate for improvement of product titers and yields of bioprocesses, because methanol has a more reduced state than sugars do and can be renewably synthesized from abundant natural gas supplies.
Yiqi Liu   +6 more
doaj   +1 more source

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