Results 151 to 160 of about 58,775 (305)

Evaluation of a prototype bioreactor based on slowly rotating drum. [PDF]

Cytotechnology
Ambrico A, Trupo M, Magarelli RA, Larocca V, Martino M, Modenese S, Vurro S.   +6 more
europepmc   +1 more source

Next‐Generation Glutamine Synthetase Expression Vector for Therapeutic Protein Manufacturing Using CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The glutamine synthetase expression system is widely used for therapeutic protein biomanufacturing in Chinese hamster ovary (CHO) cells. Here, we present the design of an enhanced glutamine synthetase vector for generating CHOK1SV GS‐KO stable pools that produce recombinant proteins at multigram‐per‐liter levels. We first developed a synthetic
Yusuf B. Johari, Sophie J. Wensley, Malak A. Ammar, Avinash Srivastava, Changhavi Ragurajan, Jenish Patel, Natalie C. Elliott, Alice C. Hodgson‐Casson, Mariano Olivera Fedi, Michael Anbar, Peter M. O'Callaghan, Bernie Sweeney   +11 more
wiley   +1 more source

Bioreactor Technology for Medicinal Plant In Vitro Cultures: Systems, Applications, and Future Perspectives. [PDF]

Biology (Basel)
Zhang S, Ma M, Liu Y, Jiang H, Gao J, Yang Q, Wei K.   +6 more
europepmc   +1 more source

Nuclease‐Free Scalable Purification of Physalis Mottle Virus‐Like Particles Expressed in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Plant virus‐like particles (VLPs) are promising nanotechnology platforms, but their translation requires purification strategies suitable for large‐scale production. Here, we developed a nuclease‐free downstream process for Physalis mottle virus (PhMV) VLPs expressed in Escherichia coli, combining ammonium sulfate precipitation, low‐pH ...
Sean K. Hsu, Patrick Opdensteinen, Tariq Hussain, Nicole F. Steinmetz   +3 more
wiley   +1 more source

Improving the reproducibility of mixing-time experiments in stirred single-use bioreactors. [PDF]

Appl Microbiol Biotechnol
Barth I, Holbeck C, Capitain C, Schirmeister T, Kampeis P.   +4 more
europepmc   +1 more source

A Hybrid Mechanistic Model Capturing Lactate Run‐Away and Back‐Metabolism in Mammalian Cell Culture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Mechanistic modeling is important in biopharmaceutical manufacturing by enabling in‐silico experimentation. However, developing a robust mechanistic model for mammalian cell culture remains challenging due to the complexity of the underlying bioprocesses.
Li Qin, James Savery, Matthew Cheeks, Suzanne S. Farid, Stephen Goldrick   +4 more
wiley   +1 more source

Production of volatile fatty acids through microbial conversion of waste sludges: influence of substrates. [PDF]

Bioresour Bioprocess
Liu L, Salminen J, Lundell T, Romantschuk M, Kontro MH.   +4 more
europepmc   +1 more source

Evaluating denitrifying bioreactors for edge-of-field nitrogen management in Iowa's tile-drained landsacpes

, 2016
Bioreactors show significant potential for removing nitrate from Iowa’s drained fields. The investigators tested the design and management of bioreactors to see what factors can make the bioreactors operate most efficiently, and how that performance ...
Bhandari, Alok, Helmers, Matt, Helmers, Matthew   +2 more
core  

Serotype‐Tailored Midstream and Downstream Operations to Advance the Manufacturing of Adeno‐Associated Viral Vectors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viruses (AAVs) are the leading vectors for in vivo gene therapy, fueling a growing demand for scalable and robust biomanufacturing processes. Yet, the impact of cell lysis conditions on the performance of midstream and downstream unit operations across serotypes remains poorly characterized.
Arianna Minzoni, Shriarjun Shastry, Eduardo Barbieri, Morgan R. Hurst, Kelvin Idanwekhai, Yuxuan Wu, Katherine Ernst, Wai‐Keen Chung, Christopher Bombeck, Saad A. Khan, Sushmita Koley, Gabriella Kiss, Oliver Rammo, Alexander Tropsha, Cristiana Boi, Michael A. Daniele, Steven Cramer, Barry Byrne, Stephen Kaminsky, Angie Snell Bennett, Stefano Menegatti   +20 more
wiley   +1 more source

Editorial: Novel trends in cultured meat research

Frontiers in Nutrition
Sishir K. Kamalapuram, Marie-Pierre Ellies-Oury, Marie-Pierre Ellies-Oury, Sghaier Chriki, Sghaier Chriki, Jean-François Hocquette, Andrew C. A. Wan, Ivana Gadjanski   +7 more
doaj   +1 more source