Results 131 to 140 of about 30,448 (259)

Disulfide bond reduction of antibodies during clarification: A bench‐scale assay and a mitigation tool

Biotechnology Progress, EarlyView.
Abstract We investigated side‐by‐side clarification with a two‐stage depth filter system against a single‐stage charge chromatographic clarifier on a number of Chinese hamster ovary (CHO) cell cultures. We found that the one‐stage fibrous anion exchange (AEX) clarification met or exceeded the performance of the conventional two‐stage depth filtration ...
Hendri Tjandra, Deepa Surendar, Isu Yoon, Florence Rusly, Yan Su, Vu Thai, Yi Liu, Ashley Hesslein   +7 more
wiley   +1 more source

The Life Cycle Assessment of Filamentous Fungi in Pharmaceutical Bioremediation and Wastewater Management: A Critical Review. [PDF]

ACS ES T Water
Dejus B, Tedoldi EL, Dejus S, Kairisa L, Rajarao GK.   +4 more
europepmc   +1 more source

Multivariate phase‐dependent optimization of bioprocesses boosts performance and quality—Why timing (of exposure) matters

Biotechnology Progress, EarlyView.
Abstract Applying a single parameter set to describe complex mammalian kinetics often is too simplistic, as it fails to capture sensitive cell‐to‐environment interactions that may be exploited to optimize production performance. To resolve this time dependency, intra‐experimental parameter shifts as part of design of dynamic experiments (DoDE) can be ...
Samuel Kienzle, Lisa Junghans, Anja Wittmann, Stefan Wieschalka, Ralf Takors, Nicole Erika Radde, Beate Presser, Verena Nold   +7 more
wiley   +1 more source

Investigation of Flow Characteristics in a Stirred-Tank Bioreactor with Flexible Blades via Integrated PIV and Image Recognition. [PDF]

Bioengineering (Basel)
Xu W, Cai C, Li Z, Lu H, Yang C, Liu B.
europepmc   +1 more source

On‐deck pH measurement with integrated feedback control on a micro‐scale platform for monoclonal antibody low pH viral inactivation

Biotechnology Progress, EarlyView.
Integrating an automated, on‐deck pH measurement method with feedback control enables precise, real‐time pH adjustment during low pH viral inactivation on a micro‐scale platform. Abstract The increasing demand for efficient monoclonal antibody manufacturing has accelerated the adoption of high throughput process development (HTPD) platforms, which ...
Paras Sharma, Nikolas von den Eichen, Sabine Schweisgut, Lars Robbel, Michael Schmitt, Daniel G. Bracewell   +5 more
wiley   +1 more source

MUC2 shifts the metabolic profiles of commensal bacteria in defined microbial communities. [PDF]

Microbiome Res Rep
Chard E, Horvath AE, Grozis M, do Nascimento RR, Baker PRS, Gorti SKK, Proos R, Horvath TD, Engevik MA.   +8 more
europepmc   +1 more source

Machine learning‐assisted clone selection for intensified cell culture processes

Biotechnology Progress, EarlyView.
Abstract Intensified fed‐batch processes are becoming increasingly prevalent among biomanufacturers due to their superior space–time yields relative to traditional, non‐intensified fed‐batch processes. However, the shift towards intensified manufacturing has unexpectedly made optimal clone selection more challenging.
Nicolas Wolnick, John Schmitt, Christian Siltanen, Julia Armstrong, Shannon Hendrick, Ariel Atchley, Brandon Downey   +6 more
wiley   +1 more source

Mechanobiology-based strategies for the maturation of biofabricated cartilage constructs. [PDF]

Front Bioeng Biotechnol
López-González I, Campillo N, Baena JM.   +2 more
europepmc   +1 more source

β‐1,4‐galactosyltransferase 1 inhibitors modulate cellular N‐glycan profiles

Biotechnology Progress, EarlyView.
Graphical representation of the study workflow used to investigate the effects of known in vitro galactosyltransferase inhibitors on cell culture performance and recombinant IgG galactosylation. Abstract N‐glycosylation is a key post‐translational modification of proteins influencing their physicochemical properties as well as biological activity. This
Jaka Kranjc, Stane Pajk, Marko Anderluh, Anja Pišlar, Matjaž Brinc   +4 more
wiley   +1 more source

Bioreactors [PDF]

Nature Biotechnology, 1995
openaire   +1 more source