Results 161 to 170 of about 111,398 (363)
Engineering in Life Sciences, Volume 23, Issue 1, January 2023., 2023 Abstract Rationally designed synthetic microbial consortia carry a vast potential for biotechnological applications. The application of such a consortium in a bioprocess, however, requires tight and individual controllability of the involved microbes.
Franziska Kratzl +2 more
wiley +1 more source
Sigma Factors as Potential Targets to Enhance Recombinant Protein Expression
Biotechnology and Bioengineering, EarlyView.ABSTRACT The transcriptional factors control the expression of many genes and represent an important layer of complexity in cell factories. However, the effect of individual sigma factor deletions from a biomanufacturing perspective has not been addressed. In this contribution, growth, green fluorescence protein (GFP) expression, and oxygen consumption
Laura Pohlen +6 more
wiley +1 more source
Assessing the growth kinetics and stoichiometry of Escherichia coli at the single‐cell level
Engineering in Life Sciences, Volume 23, Issue 1, January 2023., 2023 Abstract Microfluidic cultivation and single‐cell analysis are inherent parts of modern microbial biotechnology and microbiology. However, implementing biochemical engineering principles based on the kinetics and stoichiometry of growth in microscopic spaces remained unattained.
Katharina Smaluch +5 more
wiley +1 more source
Airlift Bioreactors - Bioreactors Design
, 2011 Producción ...
Guieysse, Benoit +2 more
openaire +1 more source
Bayesian Optimization in Bioprocess Engineering—Where Do We Stand Today?
Biotechnology and Bioengineering, EarlyView.ABSTRACT Bayesian optimization is a stochastic, global black‐box optimization algorithm. By combining Machine Learning with decision‐making, the algorithm can optimally utilize information gained during experimentation to plan further experiments—while balancing exploration and exploitation.
Florian Gisperg +5 more
wiley +1 more source
Engineering in Life Sciences, Volume 23, Issue 1, January 2023., 2023 Abstract In nature, microorganisms often reside in symbiotic co‐existence providing nutrition, stability, and protection for each partner by applying “division of labor.” This principle may also be used for the overproduction of targeted compounds in bioprocesses.
Tobias Müller +4 more
wiley +1 more source
Perfusion‐Based Production of rAAV via an Intensified Transient Transfection Process
Biotechnology and Bioengineering, EarlyView.ABSTRACT Increasing demand for recombinant adeno‐associated virus (rAAV)‐based gene therapies necessitates increased manufacturing production. Transient transfection of mammalian cells remains the most commonly used method to produce clinical‐grade rAAVs due to its ease of implementation.
Tam N. T. Nguyen +11 more
wiley +1 more source
An analysis of organism lifelines in an industrial bioreactor using Lattice‐Boltzmann CFD
Engineering in Life Sciences, Volume 23, Issue 1, January 2023., 2023 Abstract Euler‐Lagrange CFD simulations, where the biotic phase is represented by computational particles (parcels), provide information on environmental gradients inside bioreactors from the microbial perspective. Such information is highly relevant for reactor scale‐down and process optimization.
Cees Haringa
wiley +1 more source
Bioreactor Development for Production of Viral Pesticides or Heterologous Proteins in Insect Cell Culturesa [PDF]
, 1990 Michael L. Shuler +7 more
openalex +1 more source
Single Cell Protein Production From Ethanol: Model‐Based Bioreactor Operation at Industrial Scale
Biotechnology and Bioengineering, EarlyView.ABSTRACT Alternative fermentation feedstocks such as ethanol can be produced from CO2 via electrocatalytic processes that coproduce O2. In this study, industrial‐scale fermentation of ethanol with pure O2 for single cell protein (SCP) production was studied using a modeling approach.
Eduardo Almeida Benalcázar +6 more
wiley +1 more source