Results 21 to 30 of about 117,129 (304)

Thoughts on biosafety education of experimental course of pathogenic biology under COVID-19 epidemic situation [PDF]

Jichu yixue yu linchuang, 2020
The current COVID-19 epidemic situation is grim, and the government has realized the importance of biosafety. This paper summarizes the situation and experience of biosafety management and education in pathogenic biology teaching laboratory from the ...
ZHANG Zhuo-yang, YUAN Zhen-dong, YANG Yang, YANG He-ping, XIAO Jia-qi, ZHAO Wei
doaj  

Genome-scale analyses of transcriptional start sites in Mycobacterium marinum under normoxic and hypoxic conditions

BMC Genomics, 2021
Background Hypoxic stress plays a critical role in the persistence of Mycobacterium tuberculosis (Mtb) infection, but the mechanisms underlying this adaptive response remain ill defined. Material and methods In this study, using M. marinum as a surrogate,
Shaojia Huang, Wei Zhou, Wei Tang, Yong Zhang, Yangbo Hu, Shiyun Chen   +5 more
doaj   +1 more source

The study of biosafety risk identification and analysis for facilities in biosafety level 3 laboratories

Frontiers in Cellular and Infection Microbiology
ObjectiveThe aim of this study was to identify and analyze biosafety risk points in biosafety level 3 (BSL-3) laboratory facilities to bring awareness to the attention of administrative staff, reduce the biosafety risks, and improve the risk management ...
Bingwang Lv, Zhiyu Zhou, Jinsong Wang, Qun Niu, Yuchen Shi, Yanbo Sun, Hongda Zhao, Dongsheng Lu, Tao Fan, Yang Huang   +9 more
doaj   +1 more source

New Frontiers in Biosafety and Biosecurity [PDF]

Frontiers in Bioengineering and Biotechnology, 2021
Biotechnology has great potential to contribute to sustainable development. Over the past 18 months, it has enabled rapid deployment of methods to detect, treat and protect people against infection by SARS-CoV-2 (Baek et al., 2020; Beigel et al., 2020; Voysey et al., 2021). In addition, gene editing is promising to revolutionize medicine, public health,
openaire   +3 more sources

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Assessment of the Biosafety and Biosecurity Landscape in the Philippines and the Development of the National Biorisk Management Framework

, 2021
Introduction: The emergence of biological threats that can potentially affect millions emphasizes the need to develop a policy framework in the Philippines that can mount an adequate and well-coordinated response. The objective of the study was to assess,
Destura, Raul V., Lopez, Jaifred Christian F., Lam, Hilton Y, Sales, Reneepearl Kim P, Gomez, Ma. Ida Faye A., dela Tonga, Angelo, Navarro, Rohani C, Gomez, Ma Ida Faye A, Destura, Raul V, Sales, Reneepearl Kim P., Lam, Hilton Y., Ulanday, Gianne Eduard, Lopez, Jaifred Christian F, Navarro, Rohani C.   +13 more
core   +1 more source

Integration of biosafety surveillance through Biosafety Surveillance Conceptual Data Model

Biosafety and Health, 2019
The global movement of people and goods has increased the risk of biosecurity threats and their potential to induce large economic, social, and environmental harm.
Songwang Wang, Yangfei Li, Qiang Chen, Xiaoyu Feng, Xiju Shi, Ying Huang, Li Mei, Wei Li, Haizhou Liu, Xiaopeng Qi, Di Liu   +10 more
doaj   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Comprehensive Characterization of 98 Chinese Cases of Genetic Creutzfeldt‐Jakob Disease With T188K Mutation

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To characterize the demographic, clinical, and laboratory features of the Chinese patients of genetic Creutzfeldt‐Jakob disease with T188K variant (T188K‐gCJD), the most common subtype of genetic prion diseases (gPrDs) in China. Methods In this nationwide retrospective study, data from 98 genetically confirmed T188K‐gCJD patients ...
Chun‐Jie Li, Dong‐Lin Liang, Li‐Ping Gao, Wei‐Wei Zhang, Wei Zhou, A. Ruhan, Bing Xu, Run‐Dong Cao, Kang Xiao, Cao Chen, Qi Shi, Xiao‐Ping Dong   +11 more
wiley   +1 more source