Results 71 to 80 of about 102,201 (258)

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Protocol for near-infrared optogenetics manipulation of neurons and motor behavior in C. elegans using emissive upconversion nanoparticles

STAR Protocols
Summary: In deep tissue, optogenetics faces limitations with visible light. Here, we present a protocol for near-infrared (NIR) optogenetics manipulation of neurons and motor behavior in Caenorhabditis elegans using emissive upconversion nanoparticles ...
Ruipeng Wang, Jingxuan Guo, Hanlu Yao, Xuekai Luo, Yixiang Deng, Yuhang Tian, Yan Zhang, Shangbang Gao   +7 more
doaj   +1 more source

New and future developments in microbial biotechnology and bioengineering [electronic resource] : recent developments in trichoderma research /

, 2020
New and Future Developments in Microbial Biotechnology and Bioengineering: Recent Developments in Trichoderma Research covers topics on-Trichoderma biodiversity, strain improvement and related researches in bioprocess technology, chemical engineering ...
Singh, H. B.,Dr., Gupta, Vijai Kumar., Druzhinina, Irina S., Zeilinger-Migsich, Susanne.   +3 more
core  

Mechatronic design methodology for biotechnology products

, 2009
Biotechnology products can be divided into (1) biologics, which comprise those metabolites, biopolymers, and cell structures that are produced through biological processes, and (2) biotechnical machines, which are apparatuses and devices that transform ...
Mandenius, Carl-Fredrik,, Björkman, Mats,   +1 more
core   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

3D-Printed PCL/PPy Conductive Scaffolds as Three-Dimensional Porous Nerve Guide Conduits (NGCs) for Peripheral Nerve Injury Repair

, 2021
10.3389/fbioe.2019.00266FRONTIERS IN BIOENGINEERING AND ...
Lu, Wen Feng, Fuh, Jerry YH, Cao, Tong, Vijayavenkataraman, Sanjairaj, Sriram, Gopu, Kannan, Sathya   +5 more
core   +1 more source

The heterodimeric amino acid transporters (HAT) of the SLC7/SLC3 family: A structure−function relationships and relevance to human pathology

FEBS Letters, EarlyView.
Heterodimeric amino acid transporters consist of SLC7 and SLC3 family proteins arranged in a conserved structural organization. They regulate nutrient transport across cell membranes, supporting essential cellular functions. These transporters also contribute to xenobiotic/drug uptake and distribution.
Mariafrancesca Scalise, Tiziano Mazza, Raffaella Scanga, Lara Console, Michele Galluccio, Cesare Indiveri   +5 more
wiley   +1 more source

Protocol for large DNA transgenesis in mice using the Cas9+Bxb1 toolbox

STAR Protocols
Summary: Precise integration of DNA constructs greater than 3 kb into mouse zygotes is difficult. Here, we present a protocol for large DNA transgenesis in mice using the Cas9+Bxb1 toolbox.
Valerie Erhardt, Chrystal M. Snow, Vishnu Hosur   +2 more
doaj   +1 more source

pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis

, 2021
10.3389/fbioe.2020.00412FRONTIERS IN BIOENGINEERING AND ...
Ding, Jeak Ling, Ong, Tiffany T, Koean, Ricky, Tam, John Kit Chung, Ang, Zhiwei, Verma, Riva   +5 more
core   +1 more source

Neutralization of the PTX3/CD44 axis suppresses TGF‐β1 signaling in kidney fibrosis

FEBS Letters, EarlyView.
This study investigates how the PTX3/CD44 axis promotes kidney fibrosis by activating TGF‐β1 signaling. We demonstrate that the neutralizing antibody WHC‐001 effectively inhibits this pathway, suppressing myofibroblast activation and partial epithelial–mesenchymal transition (EMT).
Chao‐Chun Cheng, Shen‐Shin Chang, Feng‐Wei Chen, Ying‐Chieh Su, Thanh Phuong Nguyen, Yu‐Wei Hsiao, Jhih‐Ying Chi, Hsin‐Yin Liang, Ju‐Ming Wang   +8 more
wiley   +1 more source