Results 81 to 90 of about 102,201 (258)

Protocol for eVLP-mediated CRISPR-Cas9 and prime editing in pancreatic cancer cell lines

STAR Protocols
Summary: Pancreatic ductal adenocarcinoma (PDAC) cell lines resist transfection, limiting gene-editing experiments. Here, we present a protocol for delivering CRISPR-Cas9 or prime editors into PDAC cell lines using retrovirus-derived engineered virus ...
Chloe Timmins-Young, Marco Cordani, Elisa Giovannetti, Despoina Aslanoglou, Alvaro Gutierrez, Eithne Costello, Mark Glenn   +6 more
doaj   +1 more source

Current Developments in Biotechnology and Bioengineering: Advanced Membrane Separation Processes for Sustainable Water and Wastewater Management - Aerobic Membrane Bioreactor Processes and Technologies

, 2020
Current Developments in Biotechnology and Bioengineering: Advanced Membrane Separation Processes for Sustainable Water and Wastewater Management - Aerobic Membrane Bioreactor Processes and Technologies consolidates up-to-date research developments in ...
Mannina G., Ng T. C. A., Ng H. Y., Ng H.Y., Pandey, A., Ngo, H.H., Mannina, G., Ngo H. H., Ng, T.C.A., Ngo H.H., Guo W., Ng, H.Y., Pandey A., Ng, HY, Guo, W, Mannina, G, Ngo, HH, Pandey, A   +17 more
core   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

Protocol for 3D bioprinting of nanoparticle-laden hydrogels to enhance antibacterial and imaging properties

STAR Protocols
Summary: This protocol describes the preparation of a nanoparticle-encapsulated bioink capable of protecting tissue-engineered constructs against bacterial infections while also providing contrast for magnetic resonance (MR) imaging modalities.
Mehdi Salar Amoli, Linqi Jin, Sarah Rezapourdamanab, Maher Saadeh, Yamini Singh, Liqun Ning, Boeun Hwang, Martin L. Tomov, Christopher N. LaRock, Morteza Mahmoudi, Holly Bauser-Heaton, Vahid Serpooshan   +11 more
doaj   +1 more source

Kinetic investigation of a solvent-free, chemoenzymatic reaction sequence towards enantioselective synthesis of a ß-amino acid ester

, 2012
Strompen S, Weiß M, Ingram T, et al. Kinetic investigation of a solvent-free, chemoenzymatic reaction sequence towards enantioselective synthesis of a ß-amino acid ester. Biotechnology and Bioengineering.
Weiß, Markus, Lutz Hilterhaus, Smirnova, I., Liese, Andreas, Ingram, Thomas, Strompen, S., Hilterhaus, L., Hilterhaus, Lutz, Andreas Liese, Strompen, Simon, Markus Weiß, Simon Strompen, Gröger, Harald, Irina Smirnova, Ingram, T., Liese, A., Harald Gröger, Weiß, M., Thomas Ingram, Smirnova, Irina   +19 more
core   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

KDM7A and KDM1A inhibition suppresses tumour promoting pathways in prostate cancer

Molecular Oncology, EarlyView.
Treatment resistance is a major challenge for patients with advanced prostate cancer. This study examined an alternative approach to target the major prostate cancer‐promoting pathway by targeting epigenetic factors, whose levels are higher in tumours.
Jennie N Jeyapalan, Veronika M Metzler, Simone de Brot, Corinne L Woodcock, Anna E Harris, Jennifer Lothion‐Roy, Emeli M Nilsson, Atara Ntekim, Michael S Toss, Jenny L Persson, Francesca Khani, Brian D Robinson, Lorraine J Gudas, Emad Rakha, David M Heery, Catrin S Rutland, Nigel P Mongan   +16 more
wiley   +1 more source

Protocol for rolling circle amplification-based nanopore sequencing of eccDNA from cell and plasma samples

STAR Protocols
Summary: Extrachromosomal circular DNAs (eccDNAs) can drive copy number variation and genomic rearrangements and act as mobile regulators of gene expression. Here, we detail a protocol for eccDNA identification from cell and plasma samples.
Biyuan Miao, Xinlei Huang, Hui Chen, Wang Li, Qingsong Zen, Huaxi Li, Jia Gu, Pingping Xu, Shaogui Wan   +8 more
doaj   +1 more source

Analysis on the evolution and governance of the biotechnology industry of China [PDF]

, 2007
The past twenty years have witnessed the high-speed growth of China’s biotechnology industry, and this presents an excellent opportunity to examine the changes that have taken place, especially, to carry out overall evaluation and governance analysis ...
Zhu, L., Hong, J., Wang, K., Marinova, D.   +3 more
core