Probing the Catalytic Roles of Arg548 and Gln552 in the Carboxyl Transferase Domain of the \u3cem\u3eRhizobium etli\u3c/em\u3e Pyruvate Carboxylase by Site-directed Mutagenesis [PDF]
, 2010 The roles of Arg548 and Gln552 residues in the active site of the carboxyl transferase domain of Rhizobium etli pyruvate carboxylase were investigated using site-directed mutagenesis.
Adina-Zada, Abdussalam +8 more
core +1 more source
Selective targeting of proteins by hybrid polyoxometalates: Interaction between a bis-biotinylated hybrid conjugate and avidin [PDF]
, 2018 The Keggin-type polyoxometalate [\u3b3-SiW10O36]8 12 was covalently modified to obtain a bis-biotinylated conjugate able to bind avidin. Spectroscopic studies such as UV-vis, fluorimetry, circular dichroism, coupled to surface plasmon resonance technique
Alessandra Saccani +12 more
core +2 more sources
FEBS Open Bio, EarlyView.We generated and characterized clear cell renal cell carcinoma models using the patient‐derived RCC243 cell line—including cell culture, orthotopic, and metastatic tumors—via single‐cell RNA‐sequencing for comparisons between models and patient tumor datasets.
Richard Huang +9 more
wiley +1 more source
Dna2p Helicase/Nuclease Is a Tracking Protein, Like FEN1, for Flap Cleavage during Okazaki Fragment Maturation [PDF]
, 2004 During cellular DNA replication the lagging strand is generated as discontinuous segments called Okazaki fragments. Each contains an initiator RNA primer that is removed prior to joining of the strands.
Bambara, Robert A. +2 more
core +1 more source
Advanced Functional Materials, EarlyView.Oct4‐nanoscript, a biomimetic nanoparticle‐based artificial transcription factor, precisely regulates cellular rejuvenation by activating Oct4 target genes, restoring epigenetic marks, and reducing DNA damage. In a progeria model, it effectively rescued aging‐associated pathologies and extended lifespan.
Hongwon Kim +8 more
wiley +1 more source
Targeting extracellular DNA to deliver IGF-1 to the injured heart. [PDF]
, 2014 There is a great need for the development of therapeutic strategies that can target biomolecules to damaged myocardium. Necrosis of myocardium during a myocardial infarction (MI) is characterized by extracellular release of DNA, which can serve as a ...
Brown, Milton +9 more
core +2 more sources
Advanced Functional Materials, EarlyView.This review highlights recent advances in engineering artificial antigen‐presenting cells (aAPCs) as alternatives to dendritic cells for T cell expansion. Key design principles inspired by the immunological synapse are discussed, with emphasis on strategies for polyclonal and antigen‐specific T cell expansion.
Nguyen Thi Nguyen, Yu Seok Youn
wiley +1 more source
Artificial Metalloenzymes Based on the Biotin-Streptavidin Technology: Challenges and Opportunities.
Accounts of Chemical Research, 2016 The biotin-streptavidin technology offers an attractive means to engineer artificial metalloenzymes (ArMs). Initiated over 50 years ago by Bayer and Wilchek, the biotin-(strept)avidin techonology relies on the exquisite supramolecular affinity of either ...
T. Heinisch, T. Ward
semanticscholar +1 more source
The Critical Effect of Hydration on the Resonant Signatures of THz Biospectroscopy [PDF]
arXiv, 2018 Here we present an original study of the effect of hydration on Terahertz absorption signatures in biomolecular (lactose monohydrate and biotin) and bioparticles( Bacillus thuringiensis and Bacillus cereus spores). We observe "read-shift" in center frequency with increasing hydration in all samples, consistent with Lorentzian-oscillator behavior.
arxiv
Confining photonic nanojet in a microwell on microsphere lens for highly efficient light focusing, signal amplification and quantitative detection [PDF]
arXiv, 2021 Dielectric microspheres or microbeads can squeeze light into the subwavelength scale via photonic nanojet (PNJ) focusing. This enables strong light-matter interactions within its focus and induces extraordinary effects such as enhancements in light emission, signal collection and various other applications.
arxiv