Results 21 to 30 of about 456,411 (294)

Production of mutant streptavidin protein and investigation of its effect on the performance of streptavidin [PDF]

Iranian Journal of Basic Medical Sciences, 2023
Objective(s): Streptavidin is a versatile protein in cell science. The tetramer structure of streptavidin plays a key role in this binding, but this form interferes with some assays.
Elham Didevara, Shabnam Sadoogh Abbassian, Abdolrahime Sadeghi, Hamid Abtahi   +3 more
doaj   +1 more source

Structure and substrate selectivity of the 750-kDa α6β6 holoenzyme of geranyl-CoA carboxylase. [PDF]

, 2015
Geranyl-CoA carboxylase (GCC) is essential for the growth of Pseudomonas organisms with geranic acid as the sole carbon source. GCC has the same domain organization and shares strong sequence conservation with the related biotin-dependent carboxylases 3 ...
Huang, Christine S, Jurado, Ashley R, Tong, Liang, Zhang, Xing, Zhou, Z Hong   +4 more
core   +2 more sources

Determination of Nine Kinds of Vitamin B Components in Healthy Food Vitamin B Tablets by HPLC

Shipin gongye ke-ji, 2022
Objective: To establish a method for the determination of nine vitamin B components in vitamin B tablets by high performance liquid chromatography-diode array detector (HPLC-DAD). Method: Folic acid was ultrasonically extracted with 0.3% ammonia solution,
Wanqing TAN, Shuling WANG, Riqing GUAN
doaj   +1 more source

An improved method for surface immobilisation of RNA: application to small Non-Coding RNA - mRNA pairing [PDF]

, 2013
Characterisation of RNA and its intermolecular interactions is increasing in importance as the inventory of known RNA functions continues to expand. RNA-RNA interactions are central to post-transcriptional gene regulation mechanisms in bacteria, and the ...
Butt, Louise, Callaghan, Anastasia, Gowers, Darren, Henderson, Charlotte, Mardle, C., Phillips, Jack, Pickford, Andy, Roberts, Adam J., Stone, Carlanne, Vincent, H.   +9 more
core   +3 more sources

Advanced Resistance Studies Identify Two Discrete Mechanisms in Staphylococcus aureus to Overcome Antibacterial Compounds that Target Biotin Protein Ligase

Antibiotics, 2020
Biotin protein ligase (BPL) inhibitors are a novel class of antibacterial that target clinically important methicillin-resistant Staphylococcus aureus (S. aureus). In S. aureus, BPL is a bifunctional protein responsible for enzymatic biotinylation of two
Andrew J. Hayes, Jiulia Satiaputra, Louise M. Sternicki, Ashleigh S. Paparella, Zikai Feng, Kwang J. Lee, Beatriz Blanco-Rodriguez, William Tieu, Bart A. Eijkelkamp, Keith E. Shearwin, Tara L. Pukala, Andrew D. Abell, Grant W. Booker, Steven W. Polyak   +13 more
doaj   +1 more source

Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding

Nanomaterials, 2022
Proteinaceous nanovaccine delivery systems have significantly promoted the development of various high-efficiency vaccines. However, the widely used method of coupling the expression of scaffolds and antigens may result in their structural interference ...
Yixin Shi, Chao Pan, Kangfeng Wang, Yan Liu, Yange Sun, Yan Guo, Peng Sun, Jun Wu, Ying Lu, Li Zhu, Hengliang Wang   +10 more
doaj   +1 more source

A Stable Biotin-Streptavidin Surface Enables Multiplex, Label-Free Protein Detection by Aptamer and Aptamer-Protein Arrays Using Arrayed Imaging Reflectometry

Sensors, 2020
While label-free multiplex sensor technology enables “mixing and matching” of different capture molecules in principle, in practice this has been rarely (if ever) demonstrated. To fill this gap, we developed protocols for the preparation of mixed aptamer-
Alanna M. Klose, Benjamin L. Miller
doaj   +1 more source

Protein-mediated dethreading of a biotin-functionalised pseudorotaxane [PDF]

, 2014
In this article, we describe the synthesis of new biotin-functionalised naphthalene derivatives 3 and 4 and their complexation behaviour with avidin and neutravidin using a range of analytical techniques.
Caldwell, S.T., Cooke, G., Cooper, A., Fitzpatrick, B., Khaled, B., Maclean, C., Nutley, M., Rabani, G., Riehle, M., Rotello, V.M., Smith, B.O., Woisel, P.   +11 more
core   +3 more sources

Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli

BMC Biotechnology, 2010
Background Biotin is an essential enzyme cofactor that acts as a CO2 carrier in carboxylation and decarboxylation reactions. The E. coli genome encodes a biosynthetic pathway that produces biotin from pimeloyl-CoA in four enzymatic steps. The final step,
Prigge Sean T, Spalding Maroya D, Delli-Bovi Teegan A   +2 more
doaj   +1 more source

Media Supplementation with Mannitol and Biotin Enhances Squalene Production of Thraustochytrium ATCC 26185 through Increased Glucose Uptake and Antioxidative Mechanisms

Molecules, 2022
Media supplementation with exogenous chemicals is known to stimulate the accumulation of important lipids produced by microalgae and thraustochytrids.
M. Kashif Ali, Biswarup Sen, Yaodong He, Mohan Bai, Guangyi Wang   +4 more
doaj   +1 more source