Critical Role of 3'-Downstream Region of pmrB in Polymyxin Resistance in Escherichia coli BL21(DE3). [PDF]
Microorganisms, 2021 Xu F +5 more
europepmc +1 more source
Identification Epitope on VP4 Protein of Senecavirus A Recognized by a Monoclonal Antibody
Animal Research and One Health, EarlyView.This study aims to identify conserved antigenic epitopes on the SVA VP4 protein using monoclonal antibodies, providing valuable tools for further exploring its functions and developing detection methods. SVA VP4 protein was expressed in Escherichia coli and purified with High‐Affinity Ni‐Charged Resin FF.
Liang Meng +7 more
wiley +1 more source
Improvement in extracellular secretion of recombinant L-asparaginase II by Escherichia coli BL21 (DE3) using glycine and n-dodecane. [PDF]
Braz J Microbiol, 2021 Flores-Santos JC +4 more
europepmc +1 more source
Biotechnology and Applied Biochemistry, EarlyView.ABSTRACT Neurodegenerative diseases, including Alzheimer's disease (AD) and Parkinson's disease (PD), are characterized by the pathological aggregation of specific proteins such as amyloid beta (Aβ) and α‐synuclein, respectively. Early detection of these protein aggregates in biological fluids could facilitate timely diagnosis and therapeutic ...
Alexandra Dybala +4 more
wiley +1 more source
Biotechnology and Bioengineering, EarlyView.SpyCatcher‐mi3 nanoparticles displaying RBD‐SD1 from MERS‐CoV, NL140422, and HKU4 elicited robust and cross‐reactive IgG responses in mice. Only MERS‐CoV RBD‐SD1 induced neutralizing antibodies against MERS‐CoV and protected human DPP4 mice from a MERS‐CoV challenge, indicating conserved serologic but limited cross‐neutralizing epitopes.
Peter J. Halfmann +9 more
wiley +1 more source
Cloning, Expression and Evaluation of Pseudomonas Aeruginosa Exotoxin A
مجله دانشکده پزشکی اصفهان, 2014 Background: Nowadays, in many studies related to the treatment of various cancers, toxic compounds are targeted against cancer cells. One of the most effective compounds is Pseudomonas exotoxin A.
Jahangir Langari +6 more
doaj
Optimization of haloacid dehalogenase production by recombinant E. coli BL21 (DE3)/pET-hakp1 containing haloacid dehalogenase gene from Klebsiella pneumoniae ITB1 using Response Surface Methodology (RSM). [PDF]
Heliyon, 2022 Ratnaningsih E +4 more
europepmc +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Microbial production of 1,3‐butanediol (1,3‐BDO) offers a renewable route to this versatile C4 chemical. However, the low performance of CoA‐acylating butyraldehyde dehydrogenase (Bld), which contains a catalytic cysteine, limits efficient production in recombinant Escherichia coli (E. coli).
Seunghyun Cho +5 more
wiley +1 more source
Refolding of bioactive human epidermal growth factor from E. coli BL21(DE3) inclusion bodies & evaluations on its in vitro & in vivo bioactivity. [PDF]
Heliyon, 2022 Maksum IP +5 more
europepmc +1 more source
Genetically Programmed Control of Overflow Metabolism
Biotechnology and Bioengineering, EarlyView.ABSTRACT Overflow metabolism is a highly undesirable phenomenon that occurs in virtually all cell factories. Although several schemes have been applied to avoid overflow metabolism, this problem remains far from being solved. In this study, a genetic sensor for detecting overflow metabolism was designed, and its substrate specificity and reversibility ...
Lærke M. Jensen +3 more
wiley +1 more source