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Recombinant protein expression in Escherichia coli: advances and challenges

Frontiers in Microbiology, 2014
Escherichia coli is the organism of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform.
Germán Leandro Rosano, Germán Leandro Rosano, Eduardo A. Ceccarelli, Eduardo A. Ceccarelli   +3 more
doaj   +3 more sources

Genomic island-encoded regulatory proteins in enterohemorrhagic Escherichia coli O157:H7

Virulence
Enterohemorrhagic Escherichia coli (EHEC) is an important zoonotic pathogen that is a major cause of foodborne diseases in most developed and developing countries and can cause uncomplicated diarrhoea, haemorrhagic colitis, and haemolytic uraemic ...
Fang Wang, Hongmin Sun, Chenbo Kang, Jun Yan, Jingnan Chen, Xuequan Feng, Bin Yang   +6 more
doaj   +3 more sources

Optimization of Col H Gene Encoding Clostridium histolyticum Collagenase to Express in Escherichia coli [PDF]

Journal of Health Sciences and Surveillance System, 2022
Background: A given amino acid sequence can be encoded by a huge number of different nucleic acid sequences. These sequences, however, have proved not to be equally useful.
Seyed Mohammad Amin Mahdian, Abbasali Raz, Navid Dinparast Djadid, Hamzeh Alipour   +3 more
doaj   +1 more source

Proteins induced by aerobiosis in Escherichia coli [PDF]

Journal of Bacteriology, 1983
The role of protein induction and repression in the adaptation of Escherichia coli to changes in the supply of oxygen and other electron acceptors is only poorly understood. We have studied the changes in cellular protein composition associated with this adaptation by measuring the levels of 170 individual polypeptides produced during aerobic or ...
M W, Smith, F C, Neidhardt
openaire   +4 more sources

Enzymatic and Non-Enzymatic Response during Nitrosative Stress in Escherichia coli

Postępy Mikrobiologii, 2022
Nitrosative stress is an adverse physiological condition mediated by an excessive level of reactive nitrogen species (RNS). RNS react with the different macromolecules in vivo and result in the inactivation of these molecules.
Nath Rohan, Sengupta Swarnab, Bhattacharjee Arindam   +2 more
doaj   +1 more source

The Protein Interactome of Glycolysis in Escherichia coli [PDF]

Proteomes, 2021
Glycolysis is regulated by numerous mechanisms including allosteric regulation, post-translational modification or protein-protein interactions (PPI). While glycolytic enzymes have been found to interact with hundreds of proteins, the impact of only some of these PPIs on glycolysis is well understood. Here we investigate which of these interactions may
Shomeek Chowdhury, Stephen Hepper, Mudassir K. Lodi, Milton H. Saier, Peter Uetz   +4 more
openaire   +3 more sources

Chimeras of Escherichia coli and Mycobacterium tuberculosis single-stranded DNA binding proteins: characterization and function in Escherichia coli. [PDF]

PLoS ONE, 2011
Single stranded DNA binding proteins (SSBs) are vital for the survival of organisms. Studies on SSBs from the prototype, Escherichia coli (EcoSSB) and, an important human pathogen, Mycobacterium tuberculosis (MtuSSB) had shown that despite significant ...
Sanjay Kumar Bharti, Kervin Rex, Pujari Sreedhar, Neeraja Krishnan, Umesh Varshney   +4 more
doaj   +1 more source

Mutant MotB proteins in Escherichia coli [PDF]

Journal of Bacteriology, 1991
The MotB protein of Escherichia coli is an essential component in each of eight torque generators in the flagellar rotary motor. Based on its membrane topology, it has been suggested that MotB might be a linker that fastens the torque-generating machinery to the cell wall.
D F, Blair, D Y, Kim, H C, Berg
openaire   +2 more sources

Engineering Escherichia coli for efficient assembly of heme proteins

Microbial Cell Factories, 2023
Background Heme proteins, such as hemoglobin, horseradish peroxidase and cytochrome P450 (CYP) enzyme, are highly versatile and have widespread applications in the fields of food, healthcare, medical and biological analysis.
Jianzhong Ge, Xiaolu Wang, Yingguo Bai, Yaru Wang, Yuan Wang, Tao Tu, Xing Qin, Xiaoyun Su, Huiying Luo, Bin Yao, Huoqing Huang, Jie Zhang   +11 more
doaj   +1 more source

Production of recombinant proteins in Escherichia coli

Genetics and Molecular Biology, 2004
Attempts to obtain a recombinant protein using prokaryotic expression systems can go from a rewarding and rather fast procedure to a frustrating time-consuming experience.
Wolfgang Schumann, Luis Carlos S. Ferreira   +1 more
doaj   +1 more source