A P-type ATPase importer that discriminates between essential and toxic transition metals [PDF]
Transition metals, although being essential cofactors in many physiological processes, are toxic at elevated concentrations. Among the membrane-embedded transport proteins that maintain appropriate intracellular levels of transition metals are ATP-driven
A. T. Lee +25 more
core +2 more sources
Escherichia coli periplasmic protein FepB binds ferrienterobactin [PDF]
Most high-affinity systems for iron uptake in Gram-negative bacteria are thought to employ periplasmic-binding-protein-dependent transport. In Escherichia coli, FepB is a periplasmic protein required for uptake of iron complexed to its endogenously-synthesized siderophore enterobactin (Ent).
D L, Stephens, M D, Choe, C F, Earhart
openaire +2 more sources
Identification of YdhV as the first molybdoenzyme binding a Bis-Mo-MPT cofactor in escherichia coli [PDF]
The oxidoreductase YdhV in Escherichia coli has been predicted to belong to the family of molybdenum/tungsten cofactor (Moco/Wco)-containing enzymes. In this study, we characterized the YdhV protein in detail, which shares amino acid sequence homology ...
Dau, Holger +7 more
core +1 more source
Expression and Purification of the Arabidopsis E4 SUMO Ligases PIAL1 and PIAL2
The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group of plant E4 SUMO ligases. This protocol allows quick and simple expression of the recombinant proteins in Escherichia coli (E.
Konstantin Tomanov, Andreas Bachmair
doaj +1 more source
Isolate Specific Cold Response of Yersinia enterocolitica in Transcriptional, Proteomic, and Membrane Physiological Changes [PDF]
Yersinia enterocolitica, a zoonotic foodborne pathogen, is able to withstand low temperatures. This psychrotrophic ability allows it to multiply in food stored in refrigerators. However, little is known about the Y. enterocolitica cold response.
Alter, Thomas +4 more
core +1 more source
Investigating the Role of Escherichia coli Infection in the Pathogenesis of Bladder Cancer: A Comparative Genomics Interaction [PDF]
Background: Bladder cancer (BC) is the 10th most common cancer worldwide. Microorganisms, including bacteria, may contribute to urological tumor development.
Mahya Fouladvandi +2 more
doaj +1 more source
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source
Thiamine-Binding Protein of Escherichia coli [PDF]
The ability to transport thiamine in Escherichia coli was reduced by osmotic shock treatment with a concomitant release of a thiamine-binding protein; its formation was repressed by thiamine added to the growth medium.
A, Iwashima, A, Matsuura, Y, Nose
openaire +2 more sources
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Isolation of Inner Membrane Vesicles from Escherichia coli by Using an Affinity Tag
This protocol was developed in a project aimed to identify the inner membrane proteins localizing to cell poles in Escherichia coli (E. coli). By using a known polar protein Tar as a tag, we isolated pole-derived inner membrane vesicles by affinity ...
Gang Li, Kevin Young
doaj +1 more source

