Results 41 to 50 of about 492,895 (317)

Repetitive N-WASP–binding elements of the Enterohemorrhagic Escherichia coli Effector EspFU Synergistically Activate Actin Assembly [PDF]

open access: yes, 2008
Enterohemorrhagic Escherichia coli (EHEC) generate F-actin–rich adhesion pedestals by delivering effector proteins into mammalian cells. These effectors include the translocated receptor Tir, along with EspFU, a protein that associates indirectly with ...
McGhie, E.J.   +40 more
core   +1 more source

Expression and Purification of the Arabidopsis E4 SUMO Ligases PIAL1 and PIAL2

open access: yesBio-Protocol, 2015
The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group of plant E4 SUMO ligases. This protocol allows quick and simple expression of the recombinant proteins in Escherichia coli (E.
Konstantin Tomanov, Andreas Bachmair
doaj   +1 more source

Investigating the Role of Escherichia coli Infection in the Pathogenesis of Bladder Cancer: A Comparative Genomics Interaction [PDF]

open access: yesMiddle East Journal of Cancer
Background: Bladder cancer (BC) is the 10th most common cancer worldwide. Microorganisms, including bacteria, may contribute to urological tumor development.
Mahya Fouladvandi   +2 more
doaj   +1 more source

Crystal Structure of the Escherichia coli Fic Toxin-Like Protein in Complex with Its Cognate Antitoxin [PDF]

open access: yes, 2016
FIC domain proteins mediate post-translational modifications of target proteins, which typically results in their inactivation. Depending on the conservation of crucial active site residues, the FIC fold serves as structural scaffold for various ...
Stanger, Frédéric V.   +11 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

open access: yesFEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe   +3 more
wiley   +1 more source

dnaX36 mutator of Escherichia coli: effects of the tau subunit of the DNA polymerase III holoenzyme on chromosomal DNA replication fidelity. [PDF]

open access: yes, 2011
The Escherichia coli dnaX36 mutant displays a mutator effect, reflecting a fidelity function of the dnaX-encoded τ subunit of the DNA polymerase III (Pol III) holoenzyme.
Jonczyk, Piotr   +7 more
core   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

open access: yesFEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li   +2 more
wiley   +1 more source

Isolation of Inner Membrane Vesicles from Escherichia coli by Using an Affinity Tag

open access: yesBio-Protocol, 2012
This protocol was developed in a project aimed to identify the inner membrane proteins localizing to cell poles in Escherichia coli (E. coli). By using a known polar protein Tar as a tag, we isolated pole-derived inner membrane vesicles by affinity ...
Gang Li, Kevin Young
doaj   +1 more source

Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli

open access: yes, 1989
Friehs K, Bailey JE. Unusual observations during construction of a new cloning vector providing lon gene expression in Escherichia coli. Journal of Biotechnology. 1989;9(4):305-316.The plasmid pJMC40 containing the lon gene was transformed into E.
Bailey, J. E., Friehs, Karl
core   +1 more source

Mutation rate plasticity in rifampicin resistance depends on Escherichia coli cell–cell interactions [PDF]

open access: yes, 2014
Variation of mutation rate at a particular site in a particular genotype, in other words mutation rate plasticity (MRP), can be caused by stress or ageing. However, mutation rate control by other factors is less well characterized.
John A. D. Aston   +31 more
core   +1 more source

Home - About - Disclaimer - Privacy