Results 31 to 40 of about 460,979 (267)
Essential Paralogous Proteins as Potential Antibiotic Multitargets in Escherichia coli
Microbiology Spectrum, 2022 Antimicrobial resistance threatens our current standards of care for the treatment and prevention of infectious disease. Antibiotics that have multiple targets have a lower propensity for the development of antibiotic resistance than those that have ...
Christine D. Hardy
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Active Protein Aggregates Produced in Escherichia coli [PDF]
International Journal of Molecular Sciences, 2011 Since recombinant proteins are widely used in industry and in research, the need for their low-cost production is increasing. Escherichia coli is one of the best known and most often used host organisms for economical protein production. However, upon over-expression, protein aggregates called inclusion bodies (IBs) are often formed. Until recently IBs
Peternel, Špela, Komel, Radovan
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Expression and Purification of the Arabidopsis E4 SUMO Ligases PIAL1 and PIAL2
Bio-Protocol, 2015 The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group of plant E4 SUMO ligases. This protocol allows quick and simple expression of the recombinant proteins in Escherichia coli (E.
Konstantin Tomanov, Andreas Bachmair
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Investigating the Role of Escherichia coli Infection in the Pathogenesis of Bladder Cancer: A Comparative Genomics Interaction [PDF]
Middle East Journal of CancerBackground: Bladder cancer (BC) is the 10th most common cancer worldwide. Microorganisms, including bacteria, may contribute to urological tumor development.
Mahya Fouladvandi +2 more
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SYNTHESIS OF NASCENT PROTEIN BY RIBOSOMES IN ESCHERICHIA COLI [PDF]
Proceedings of the National Academy of Sciences, 1959 Cells and tissues of all kinds of living organisms have been found to contain ribonucleic acid (RNA) and protein both as separate constituents and as complexes in the form of ribonucleoprotein particles. From a variety of experimental data it has been inferred that these particles are probably intimately involved in the processes of protein synthesis.
McQuillen, Kenneth +2 more
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Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
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Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
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Isolation of Inner Membrane Vesicles from Escherichia coli by Using an Affinity Tag
Bio-Protocol, 2012 This protocol was developed in a project aimed to identify the inner membrane proteins localizing to cell poles in Escherichia coli (E. coli). By using a known polar protein Tar as a tag, we isolated pole-derived inner membrane vesicles by affinity ...
Gang Li, Kevin Young
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Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations
FEBS Letters, EarlyView.The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas +6 more
wiley +1 more source