Results 21 to 30 of about 460,979 (267)

Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli

open access: yesBio-Protocol, 2022
This protocol describes the recombinant expression of proteins in E. coli containing phosphoserine (pSer) installed at positions guided by TAG codons. The E.
Phillip Zhu, Ryan Mehl, Richard Cooley
doaj   +1 more source

Producción de proteínas recombinantes de Plasmodium falciparum en Escherichia coli

open access: yesBiomédica: revista del Instituto Nacional de Salud, 2016
Introducción. La producción de proteínas recombinantes es fundamental para el estudio funcional de las proteínas de Plasmodium falciparum. Sin embargo, las proteínas recombinantes de P.
Ángela Patricia Guerra   +3 more
doaj   +1 more source

Genes and proteins of Escherichia coli (GenProtEc) [PDF]

open access: yesNucleic Acids Research, 1996
GenProtEc is a database of Escherichia coli genes and their gene products, classified by type of function and physiological role and with citations to the literature for each. Also present are data on sequence similarities among E.coli proteins with PAM values, percent identity of amino acids, length of alignment and percent aligned.
Monica Riley, David B. Space
openaire   +2 more sources

Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells

open access: yesNature Communications, 2016
Several proteins are expressed at too low abundance in the Escherichia coli (E. coli) proteome to be detected by standard methods. Here, the authors create a microfluidics-based platform enabling single-molecule counting of low-abundance proteins by ...
Burak Okumus   +11 more
doaj   +1 more source

Quantitative inner membrane proteome datasets of the wild-type and the Δmin mutant of Escherichia coli

open access: yesData in Brief, 2016
This article presents data that were obtained through measuring the impact of the Min oscillation on membrane proteins in Escherichia coli by quantitative protemoics analysis.
Suh-Yuen Liang   +3 more
doaj   +1 more source

Proteomic dataset: Profiling of membrane fraction of Escherichia coli isolated from Crohn's disease patients after adhesion and invasion experiments

open access: yesData in Brief, 2019
Crohn's disease (CD) is a type of inflammatory bowel disease (IDB). The endoscopic picture of Crohn's disease includes thickened submucosa, transmural inflammation, fissuring ulceration, and non-caseating granulomas.
Olga Bukato   +7 more
doaj   +1 more source

Protein Mobility in the Cytoplasm of Escherichia coli [PDF]

open access: yesJournal of Bacteriology, 1999
ABSTRACT The rate of protein diffusion in bacterial cytoplasm may constrain a variety of cellular functions and limit the rates of many biochemical reactions in vivo. In this paper, we report noninvasive measurements of the apparent diffusion coefficient of green fluorescent protein (GFP) in the cytoplasm of ...
Elowitz, Michael B.   +4 more
openaire   +3 more sources

Escherichia coli-derived virus-like particles in vaccine development

open access: yesnpj Vaccines, 2017
Recombinant virus-like particle-based vaccines are composed of viral structural proteins and mimic authentic native viruses but are devoid of viral genetic materials. They are the active components in highly safe and effective vaccines for the prevention
Xiaofen Huang   +4 more
doaj   +1 more source

Rescue of Escherichia coli auxotrophy by de novo small proteins

open access: yeseLife, 2023
Increasing numbers of small proteins with diverse physiological roles are being identified and characterized in both prokaryotic and eukaryotic systems, but the origins and evolution of these proteins remain unclear.
Arianne M Babina   +8 more
doaj   +1 more source

Acid shock proteins of Escherichia coli [PDF]

open access: yesFEMS Microbiology Letters, 1990
Synthesis of total cellular proteins of Escherichia coli was studied after transfer of cultures from pH 6.9 to pH 4.3. Proteins induced by such an external pH shift down were identified by mono- and bi-dimensional electrophoresis. 30 to 45 min after an acid shift, a group of at least sixteen polypeptides was markedly induced. Four of these polypeptides
M, Heyde, R, Portalier
openaire   +2 more sources

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