Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells. [PDF]
PLoS ONE, 2010 A previously engineered Methanocaldococcus jannaschii tRNA(CUA Tyr)-tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells.
Gabrielle Nina Thibodeaux +6 more
doaj +1 more source
Proteins induced by aerobiosis in Escherichia coli [PDF]
Journal of Bacteriology, 1983 The role of protein induction and repression in the adaptation of Escherichia coli to changes in the supply of oxygen and other electron acceptors is only poorly understood. We have studied the changes in cellular protein composition associated with this adaptation by measuring the levels of 170 individual polypeptides produced during aerobic or ...
M W, Smith, F C, Neidhardt
openaire +4 more sources
Engineered Recombinant Protein Production Systems Originated from Escherichia coli
Trends in Peptide and Protein Sciences, 2019 Selection of a suitable host strain is an important step in recombinant production of heterologous proteins. As the prokaryotic systems and especially Escherichia coli (E.
Javad Ranjbari
doaj +1 more source
Cloning of the C-terminal cytoplasmic fragment of the tar protein and effects of the fragment on chemotaxis of Escherichia coli [PDF]
, 1988 A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies.
Mutoh, Norihiro +2 more
core +1 more source
Active Protein Aggregates Produced in Escherichia coli [PDF]
International Journal of Molecular Sciences, 2011 Since recombinant proteins are widely used in industry and in research, the need for their low-cost production is increasing. Escherichia coli is one of the best known and most often used host organisms for economical protein production. However, upon over-expression, protein aggregates called inclusion bodies (IBs) are often formed. Until recently IBs
Peternel, Špela, Komel, Radovan
openaire +3 more sources
Escherichia coli TatA and TatB Proteins Have N-out, C-in Topology in Intact Cells [PDF]
, 2012 The twin arginine protein transport (Tat) system translocates folded proteins across the cytoplasmic membrane of prokaryotes and the thylakoid membrane of chloroplasts.
Alami +58 more
core +3 more sources
Image-based dynamic phenotyping reveals genetic determinants of filamentation-mediated beta-lactam tolerance [PDF]
, 2020 Antibiotic tolerance characterized by slow killing of bacteria in response to a drug can lead to treatment failure and promote the emergence of resistance.
Camacho, Rafael +8 more
core +2 more sources
Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli
Bio-Protocol, 2022 This protocol describes the recombinant expression of proteins in E. coli containing phosphoserine (pSer) installed at positions guided by TAG codons. The E.
Phillip Zhu, Ryan Mehl, Richard Cooley
doaj +1 more source
Transcriptional profiling of colicin-induced cell death of Escherichia coli MG1655 identifies potential mechanisms by which bacteriocins promote bacterial diversity [PDF]
, 2004 We report the transcriptional response of Escherichia coli MG1655 to damage induced by colicins E3 and E9, bacteriocins that kill cells through inactivation of the ribosome and degradation of chromosomal DNA, respectively. Colicin E9 strongly induced the
Hinton, Jay C. D. +6 more
core +2 more sources
Data in Brief, 2016 This article presents data that were obtained through measuring the impact of the Min oscillation on membrane proteins in Escherichia coli by quantitative protemoics analysis.
Suh-Yuen Liang +3 more
doaj +1 more source