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Recombinant protein expression in Escherichia coli: advances and challenges
Frontiers in Microbiology, 2014 Escherichia coli is the organism of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform.
Germán Leandro Rosano +3 more
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Mutant MotB proteins in Escherichia coli [PDF]
Journal of Bacteriology, 1991 The MotB protein of Escherichia coli is an essential component in each of eight torque generators in the flagellar rotary motor. Based on its membrane topology, it has been suggested that MotB might be a linker that fastens the torque-generating machinery to the cell wall.
D F, Blair, D Y, Kim, H C, Berg
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Producing GST-Cbx7 Fusion Proteins from Escherichia coli
Bio-Protocol, 2017 This protocol describes the production of GST-Cbx7 fusion proteins from E. coli, originally developed in the recent publication (Zhen et al., 2016). The pGEX-6P-1-GST plasmids encoding the Cbx7 variants were transformed into BL21 competent cells.
Thao Huynh, Xiaojun Ren
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Microorganisms, 2022 Colibacillosis caused by pathogenic Escherichia coli (E. coli) is one of the most serious infectious diseases, causing an extensive burden on animal husbandry and the human healthcare system. Vaccination is one of the ideal ways to prevent E.
Guihong Pen +5 more
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Mechanisms of Intestinal Epithelial Barrier Dysfunction by Adherent-Invasive Escherichia coli. [PDF]
, 2016 Pathobiont expansion, such as that of adherent-invasive Escherichia coli (AIEC), is an emerging factor associated with inflammatory bowel disease. The intestinal epithelial barrier is the first line of defense against these pathogens.
McCole, Declan F, Shawki, Ali
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Recombinant protein production data after expression in the bacterium Escherichia coli
Data in Brief, 2016 Fusion proteins have become essential for the expression and purification of recombinant proteins in Escherichia coli. The metal-binding protein CusF has shown several features that make it an attractive fusion protein and affinity tag: ''Expression and ...
J. Enrique Cantu-Bustos +5 more
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Microorganisms, 2022 Escherichia coli (E. coli) is one of the most common pathogenic bacteria worldwide. Avian pathogenic E. coli (APEC) causes severe systemic disease in poultry (Colibacillosis), and accordingly, has an extreme risk to the poultry industry and public health
Fatma Abdelrahman +6 more
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Bioinformatic and Molecular Analysis of Inverse Autotransporters from
mSphere, 2019 Proteins secreted by the type V secretion system possess multiple functions, including the capacity to mediate adhesion, aggregation, and biolfilm formation.
Kelvin G. K. Goh +4 more
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Comparative proteomics of uropathogenic Escherichia coli during growth in human urine identify UCA-like (UCL) fimbriae as an adherence factor involved in biofilm formation and binding to uroepithelial cells [PDF]
, 2015 Uropathogenic Escherichia coli (UPEC) are the primary cause of urinary tract infection (UTI) in humans. For the successful colonisation of the human urinary tract, UPEC employ a diverse collection of secreted or surface-exposed virulence factors ...
Allsopp, LP +5 more
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Acid shock proteins of Escherichia coli [PDF]
FEMS Microbiology Letters, 1990 Synthesis of total cellular proteins of Escherichia coli was studied after transfer of cultures from pH 6.9 to pH 4.3. Proteins induced by such an external pH shift down were identified by mono- and bi-dimensional electrophoresis. 30 to 45 min after an acid shift, a group of at least sixteen polypeptides was markedly induced. Four of these polypeptides
M, Heyde, R, Portalier
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