The FEBS Journal, EarlyView.Congenital dyserythropoietic anemia type I (CDA‐I) arises from mutations in Codanin1 and CDIN1. Using quantitative biophysical approaches, we show that disease‐associated mutations disrupt the CDIN1‐Codanin1 complex. Our findings provide critical insights into the molecular mechanism that links protein dysfunction to disturbing chromatin arrangement ...
Martin Stojaspal +8 more
wiley +1 more source
A supramolecular assembly of cone‐specific G‐protein and cryptochrome 4a on lipid bilayer
The FEBS Journal, EarlyView.Immobilized phospholipid bilayers on a sensor chip surface serve as membrane platform to investigate critical protein–lipid and protein–protein interaction processes by surface plasmon resonance. The putative magnetoreceptor cryptochrome 4a and the myristoylated cone‐specific G‐protein α‐subunit (Gtα) bind with high affinity to immobilized lipid ...
Ümmügülsüm Güzelsoy‐Flügge +3 more
wiley +1 more source
High-level extracellular production of an alkaline pectate lyase in E. coli BL21 (DE3) and its application in bioscouring of cotton fabric. [PDF]
3 Biotech, 2020 Zhen J +9 more
europepmc +1 more source
The FEBS Journal, EarlyView.Redox environment modulates in vitro aggregation of Ataxin‐3, the protein implicated in spinocerebellar ataxia type 3. Reducing conditions stabilize native monomers and prevent aggregation, whereas oxidative conditions promote the formation of non‐native conformers and disulfide‐linked oligomers within the Josephin domain (JD).
Martyna Podlasiak +10 more
wiley +1 more source
Production of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy. [PDF]
Int J Mol Sci, 2020 Schwaighofer A +7 more
europepmc +1 more source
Selective targeting of cortactin tandem repeat acetylation by human lysine deacetylases
The FEBS Journal, EarlyView.Cortactin function is regulated by acetylation at several lysine residues within its tandem repeat region. Using genetic code expansion to generate cortactin variants containing precisely defined acetylation marks, we show that HDAC6 is the primary enzyme removing these modifications, with SIRT1 and SIRT2 also acting at selected sites but with lower ...
Jan Komarek +12 more
wiley +1 more source
The FEBS Journal, EarlyView.VA molecules alter VDAC1 gating by increasing anion flow and reducing cation permeability. In cancer cells, which rely on ER‐mitochondria Ca2+ transfer and overexpress VDAC1, this imbalance triggers bioenergetic stress, ROS buildup, and mitochondrial collapse, leading to cell death.
Stefano Conti‐Nibali +6 more
wiley +1 more source
Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 (DE3): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced. [PDF]
Int J Mol Sci, 2020 Pulido IY +4 more
europepmc +1 more source
The FEBS Journal, EarlyView.Small protein MtrR is a regulator of the Mtr methyltransferase complex in Methanosarcina mazei. It binds specifically to the MtrA subunit and modulates Mtr activity in response to hydrogen (H2) availability. Deleting mtrR impairs growth in the presence but not absence of H2, indicating its role in directing methyl transfer toward an energy‐conserving ...
Tim Habenicht +6 more
wiley +1 more source
Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology. [PDF]
Heliyon, 2019 Kusuma SAF +7 more
europepmc +1 more source