Results 91 to 100 of about 133,185 (340)
Advanced Science, Volume 12, Issue 28, July 24, 2025.CRISPR/Cas9, while transformative, faces challenges in specificity, precision, delivery, accessibility, flexibility, and safety. This review addresses these limitations by highlighting strategies to reduce off‐target effects, exploring HDR‐based and alternative editing approaches, and evaluating advanced delivery mechanisms.
Muna Alariqi +8 more
wiley +1 more source
G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst
eLife, 2018 Early mouse development is regulated and accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2).
Jan J Zylicz +6 more
doaj +1 more source
Should extended blastocyst culture include Day 7?
Human Reproduction, 2018 Extended culture to the blastocyst stage is widely practised, improving embryo selection and promoting single embryo transfer. Selection of useable blastocysts typically occurs on Days 5 and 6 of embryo culture.
E. Hammond, Lynsey M Cree, D. Morbeck
semanticscholar +1 more source
Tracing and Capturing the Epiblast Pluripotency of Sheep Preimplantation Embryos
Advanced Science, EarlyView.This study performs a comprehensive single‐cell transcriptome analysis of sheep embryos from embryonic (E) day 1 to E14 to elucidate the mechanism of early lineage specification and the pluripotency changes (naïve, formative, and primed) of epiblast.
Jinying Zhang +28 more
wiley +1 more source
Establishment of a trans‐vaginal ultrasound‐guided pinpoint transfer method
Reproductive Medicine and Biology, 2018 Purpose Through the use of the trans‐vaginal ultrasound‐guided pinpoint transfer method, the aim was to ensure the safety of the embryo being transferred in cases of difficult cervixes.
Sumihide Okamoto
doaj +1 more source
A Critical Examination of the Question of Personhood in Stem Cell Research [PDF]
, 2016 Stem cell research programme has been celebrated world over as the most promising medical research in the 21st century. However, the method of stem cell research involves the use and unavoidable destruction of human embryo.
Ibanga, Diana-Abasi
core
Blastocyst-induced changes in the bovine endometrial transcriptome.
Reproduction, 2018 The objectives of this study were (i) to determine whether blastocyst-induced responses in endometrial explants were detectable after 6- or 24-h co-culture in vitro; (ii) to test if direct contact is required between embryos and the endometrial surface ...
C. Passaro +7 more
semanticscholar +1 more source
Advanced Science, EarlyView.RSPO2 and GDF9:BMP15 heterodimers—core components of oocyte‐secreted factors (OSFs)—coordinate to shape the molecular architecture of preantral granulosa cells via gene‐specific synergistic and antagonistic regulation, mediated through CTNNB1–SMAD2 signaling crosstalk.
Yingmei Wang +7 more
wiley +1 more source
Transient Sperm Starvation Improves the Outcome of Assisted Reproductive Technologies
Frontiers in Cell and Developmental Biology, 2019 To become fertile, mammalian sperm must undergo a series of biochemical and physiological changes known as capacitation. These changes involve crosstalk between metabolic and signaling pathways and can be recapitulated in vitro.
Felipe A. Navarrete +17 more
doaj +1 more source
Frontiers in Cell and Developmental Biology, 2023 Neurotrophin-4 (NT-4), a neurotrophic factor, appears to affect early embryonic development because it is secreted not only by neurons but also by oviductal and uterine epithelial cells. However, no studies have characterized the effects of NT-4 on early
Mirae Kim +15 more
doaj +1 more source